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J Biol Chem, Vol. 275, Issue 16, 11957-11963, April 21, 2000
From the Department of Dermatology, University of Texas
Southwestern Medical Center, Dallas, Texas 75235-9069
Using a subtractive cDNA cloning strategy, we
isolated previously five novel genes that were expressed abundantly by
the murine dendritic cell (DC) line XS52, but not by the J774
macrophage line. One of these genes encoded a unique, DC-associated
C-type lectin, termed "dectin-1." Here we report the
characterization of a second novel gene that was also expressed in a
DC-specific manner. Clone 1B12 encoded a type II membrane-integrated
polypeptide of 209 amino acids containing a single carbohydrate
recognition domain motif in the COOH terminus. The expression pattern
of this molecule, termed "dectin-2," was almost indistinguishable
from that for dectin-1; that is, both were expressed abundantly at mRNA and protein levels by the XS52 DC line, but not by non-DC lines, and both were detected in spleen and thymus, as well as in skin
resident DC (i.e. Langerhans cells). Interestingly, reverse transcriptase-polymerase chain reaction and immunoblotting revealed multiple bands of dectin-2 transcripts and proteins suggesting molecular heterogeneity. In fact, we isolated additional cDNA clones encoding two distinct, truncated dectin-2 isoforms. Genomic analyses indicated that a full-length dectin-2 (
Cloning of a Second Dendritic Cell-associated C-type Lectin
(Dectin-2) and Its Alternatively Spliced Isoforms*
isoform) is encoded
by 6 exons, whereas truncated isoforms (
and 
) are produced by
alternative splicing. We propose that dectin-2 and its isoforms, together with dectin-1, represent a unique subfamily of DC-associated C-type lectins.
*
This work was supported by National Institutes of Health
research Grants RO1-AR44189, RO1-AR35068, RO1-AR43777, and RO1-AI43262, Taisho Pharmaceutical Co., Ltd., Ohmiya, Japan, and in part by the
Centre de Recherches et Investigations Epidermiques et Sensorielles Award (to A. T.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 214-648-3419;
Fax: 214-648-3472; E-mail: atakas@mednet.swmed.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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