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J Biol Chem, Vol. 275, Issue 16, 12051-12060, April 21, 2000
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From the Departments of The genome of Trypanosoma cruzi
contains tandem arrays of alternating genes encoding amastin and tuzin.
Amastin is a surface glycoprotein abundantly expressed on the
intracellular mammalian amastigote form of the protozoan parasite, and
tuzin is a G-like protein. We demonstrated previously that the
amastin-tuzin gene cluster is polycistronically transcribed to an equal
extent in all parasite life cycle stages. The steady state level of
amastin mRNA, however, is 68-fold more abundant in amastigotes than
in epimastigotes. Here we show that the half-life of amastin mRNA is 7 times longer in amastigotes than in epimastigotes. Linker replacement experiments demonstrate that the middle one-third of the
630-nucleotide 3'-untranslated region (UTR) is responsible for the
amastin mRNA up-regulation. This positive effect is dependent on
the distance of the 3'-UTR segment from the stop codon and the
polyadenylation site as well as on its orientation. A protein or
protein complex more abundant in amastigotes than in epimastigotes binds to this minimally defined 3'-UTR segment and may be involved in
its regulatory function.
Biochemistry and
§ Internal Medicine, University of Iowa and the
Department of Veterans Affairs Medical Center,
Iowa City, Iowa 52242
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