|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 16, 12273-12280, April 21, 2000
From the Center for Craniofacial Molecular Biology, University of
Southern California, Los Angeles, California 90033
Amelogenin expression is ameloblast-specific and
developmentally regulated at the temporal and spatial levels. In a
previous transgenic mouse analysis, the expression pattern of the
endogenous amelogenin gene was recapitulated by a reporter gene driven
by a 2.2-kilobase mouse amelogenin proximal promoter. To understand the
molecular mechanisms underlying the spatiotemporal expression of the
amelogenin gene during odontogenesis, the mouse amelogenin promoter was
systematically analyzed in mouse ameloblast-like LS8 cells. Deletion
analysis identified a minimal promoter (
Identification of CCAAT/Enhancer-binding Protein
as a
Transactivator of the Mouse Amelogenin Gene*
70/+52) containing a
CCAAT/enhancer-binding protein (C/EBP)-binding site upstream of the
TATA box. In transient transfection assays, C/EBP
up-regulated the
promoter activity in a dose-dependent manner. The
C/EBP-binding site was necessary for both C/EBP-mediated transactivation and basal promoter activity. Electrophoresis mobility shift assays demonstrated that C/EBP bound to its cognate site in
the amelogenin promoter and that the binding was specific. Endogenous
C/EBP was detected in LS8 cells, and overexpression of exogenous
C/EBP in LS8 cells was able to increase the expression level of the
endogenous amelogenin protein. The activity of the amelogenin promoter
in rat parotid Pa-4 cells and Madin-Darby canine kidney cells was
minimal, ranging from 20 to 30% of the activity in ameloblast-like
cells. Transient transfection experiments showed that C/EBP
transactivated the mouse amelogenin reporter gene in Pa-4 cells, but
not in Madin-Darby canine kidney cells. Taken together, these data
indicate that C/EBP is a bona fide transcriptional
activator of the mouse amelogenin gene in a cell type-specific manner.
*
This work was supported by NIDCR Grant DE06988 from the
National Institutes of Health.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: CSA142, CCMB,
University of Southern California, 2250 Alcazar St., Los Angeles, CA
90033. Tel.: 323-442-3178; Fax: 323-442-2981; E-mail:
mlsnead@hsc.usc.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  X.-P. Wang, D. J. O'Connell, J. J. Lund, I. Saadi, M. Kuraguchi, A. Turbe-Doan, R. Cavallesco, H. Kim, P. J. Park, H. Harada, et al. Apc inhibition of Wnt signaling regulates supernumerary tooth formation during embryogenesis and throughout adulthood Development, June 1, 2009; 136(11): 1939 - 1949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Xu, H. Harada, and A. Taniguchi The Effects of LAMP1 and LAMP3 on M180 Amelogenin Uptake, Localization and Amelogenin mRNA Induction by Amelogenin Protein J. Biochem., October 1, 2008; 144(4): 531 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.L. Paine, M.L. Snead, H.J. Wang, N. Abuladze, A. Pushkin, W. Liu, L.Y. Kao, S.M. Wall, Y.-H. Kim, and I. Kurtz Role of NBCe1 and AE2 in Secretory Ameloblasts Journal of Dental Research, April 1, 2008; 87(4): 391 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xu, Y. L. Zhou, F. J. Gonzalez, and M. L. Snead CCAAT/Enhancer-binding Protein {delta} (C/EBP{delta}) Maintains Amelogenin Expression in the Absence of C/EBP{alpha} in Vivo J. Biol. Chem., October 12, 2007; 282(41): 29882 - 29889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xu, H. Harada, and A. Taniguchi The Exon 6ABC Region of Amelogenin mRNA Contribute to Increased Levels of Amelogenin mRNA through Amelogenin Protein-enhanced mRNA Stabilization J. Biol. Chem., October 27, 2006; 281(43): 32439 - 32444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhu, M. L. Paine, W. Luo, P. Bringas Jr., and M. L. Snead Altering Biomineralization by Protein Design J. Biol. Chem., July 28, 2006; 281(30): 21173 - 21182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, Y. L. Zhou, W. Luo, Q.-S. Zhu, D. Levy, O. A. MacDougald, and M. L. Snead NF-Y and CCAAT/Enhancer-binding Protein {alpha} Synergistically Activate the Mouse Amelogenin Gene J. Biol. Chem., June 9, 2006; 281(23): 16090 - 16098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xu, H. Harada, T. Yokohama-Tamaki, S. Matsumoto, J. Tanaka, and A. Taniguchi Reuptake of Extracellular Amelogenin by Dental Epithelial Cells Results in Increased Levels of Amelogenin mRNA through Enhanced mRNA Stabilization J. Biol. Chem., January 27, 2006; 281(4): 2257 - 2262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. L. Zhou, Y. Lei, and M. L. Snead Functional Antagonism between Msx2 and CCAAT/Enhancer-binding Protein alpha in Regulating the Mouse Amelogenin Gene Expression Is Mediated by Protein-Protein Interaction J. Biol. Chem., September 8, 2000; 275(37): 29066 - 29075. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |