| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 17, 12633-12638, April 28, 2000
From the Department of Biomedical and Surgical Sciences, Verona
University, 37134 Verona, Italy and the § Department of
Bioscience, National Cardiovascular Center Research Institute,
Fujishirodai, Suita, Osaka 565-8565, Japan
In this study we examined the effect of oxidized
low density lipoprotein (ox-LDL) on the intracellular production of
reactive oxygen species (ROS) in bovine aortic endothelial cells
(BAECs) and whether this increase occurs through its binding to the
endothelial receptor lectin-like ox-LDL receptor-1 (LOX-1).
Furthermore, this study also aimed to ascertain whether the binding of
ox-LDL to LOX-1 is associated with NF-
Oxidized Low Density Lipoprotein (ox-LDL) Binding to ox-LDL
Receptor-1 in Endothelial Cells Induces the Activation of NF-
B
through an Increased Production of Intracellular Reactive Oxygen
Species*
,
B activation. ox-LDL induced a
significant dose-dependent increase in ROS production after
a 30-s incubation with BAECs (p < 0.01). ROS
formation was markedly reduced in BAECs incubated with anti-LOX-1
monoclonal antibody (p < 0.001), while control
nonimmune IgG produced no effect. ox-LDL induced a time- and
dose-dependent significant increase in ROS formation only in CHO-K1 cells stably expressing bovine LOX-1 (p < 0.001), while no increase was present in CHO-K1 cells. The activation
of the transcription factor NF-B in BAECs was evident after a 5-min incubation with ox-LDL and was attenuated by anti-LOX-1 monoclonal antibody. The conclusion is that one of the pathophysiological consequences of ox-LDL binding to LOX-1 may be the activation of
NF-B through an increased ROS production.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dipartimento di
Scienze Biomediche e Chirurgiche, c/o Medicina D-Ospedale Policlinico, Università di Verona, 37134 Verona, Italy. Tel.: 39 045 8074806; Fax: 39 045 583041; E-mail: comina@medicinad.univr.it.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M Ethier-Chiasson, J-C Forest, Y Giguere, A Masse, C Marseille-Tremblay, E Levy, and J Lafond Modulation of placental protein expression of OLR1: implication in pregnancy-related disorders or pathologies Reproduction, October 1, 2008; 136(4): 491 - 502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. B. Tan, S. W. M. Shiu, Y. Wong, L. Leng, and R. Bucala Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus J. Lipid Res., July 1, 2008; 49(7): 1438 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Mollace, S. Ragusa, I. Sacco, C. Muscoli, F. Sculco, V. Visalli, E. Palma, S. Muscoli, L. Mondello, P. Dugo, et al. The Protective Effect of Bergamot Oil Extract on Lecitine-like OxyLDL Receptor-1 Expression in Balloon Injury-related Neointima Formation Journal of Cardiovascular Pharmacology and Therapeutics, June 1, 2008; 13(2): 120 - 129. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Bukowska, L. Schild, G. Keilhoff, D. Hirte, M. Neumann, A. Gardemann, K. H. Neumann, F.-W. Rohl, C. Huth, A. Goette, et al. Mitochondrial Dysfunction and Redox Signaling in Atrial Tachyarrhythmia Experimental Biology and Medicine, May 1, 2008; 233(5): 558 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Kelly, P. Wu, C. E. Patterson, C. Temm, and J. H. Dominguez LOX-1 and inflammation: a new mechanism for renal injury in obesity and diabetes Am J Physiol Renal Physiol, May 1, 2008; 294(5): F1136 - F1145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Namgaladze, A. Kollas, and B. Brune Oxidized LDL attenuates apoptosis in monocytic cells by activating ERK signaling J. Lipid Res., January 1, 2008; 49(1): 58 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Dominguez, J. L. Mehta, D. Li, P. Wu, K. J. Kelly, C. S. Packer, C. Temm, E. Goss, L. Cheng, S. Zhang, et al. Anti-LOX-1 therapy in rats with diabetes and dyslipidemia: ablation of renal vascular and epithelial manifestations Am J Physiol Renal Physiol, January 1, 2008; 294(1): F110 - F119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Martin-Fuentes, F. Civeira, D. Recalde, A. L. Garcia-Otin, E. Jarauta, I. Marzo, and A. Cenarro Individual Variation of Scavenger Receptor Expression in Human Macrophages with Oxidized Low-Density Lipoprotein Is Associated with a Differential Inflammatory Response J. Immunol., September 1, 2007; 179(5): 3242 - 3248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-P. Hu, A. Dandapat, Y. Liu, P. L. Hermonat, and J. L. Mehta Blockade of hypoxia-reoxygenation-mediated collagen type I expression and MMP activity by overexpression of TGF-beta1 delivered by AAV in mouse cardiomyocytes Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1833 - H1838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Okamura, J. M. Lopez-Guisa, K. Koelsch, S. Collins, and A. A. Eddy Atherogenic scavenger receptor modulation in the tubulointerstitium in response to chronic renal injury Am J Physiol Renal Physiol, August 1, 2007; 293(2): F575 - F585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cheng, R. Cui, C.-H. Chen, and J. Du Oxidized Low-Density Lipoprotein Stimulates p53-Dependent Activation of Proapoptotic Bax Leading to Apoptosis of Differentiated Endothelial Progenitor Cells Endocrinology, May 1, 2007; 148(5): 2085 - 2094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Xu, X. Gao, B. J. Potter, J.-M. Cao, and C. Zhang Anti-LOX-1 Rescues Endothelial Function in Coronary Arterioles in Atherosclerotic ApoE Knockout Mice Arterioscler. Thromb. Vasc. Biol., April 1, 2007; 27(4): 871 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Qiu, A. C. Ho, W. Yu, and J. S. Hill Suppression of endothelial or lipoprotein lipase in THP-1 macrophages attenuates proinflammatory cytokine secretion J. Lipid Res., February 1, 2007; 48(2): 385 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Chang, C.-Y. Han, T. N. Wight, and A. Chait Antioxidants Inhibit the Ability of Lysophosphatidylcholine to Regulate Proteoglycan Synthesis Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 494 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Mehta, J. Chen, P. L. Hermonat, F. Romeo, and G. Novelli Lectin-like, oxidized low-density lipoprotein receptor-1 (LOX-1): A critical player in the development of atherosclerosis and related disorders Cardiovasc Res, January 1, 2006; 69(1): 36 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Hinagata, M. Kakutani, T. Fujii, T. Naruko, N. Inoue, Y. Fujita, J. L. Mehta, M. Ueda, and T. Sawamura Oxidized LDL receptor LOX-1 is involved in neointimal hyperplasia after balloon arterial injury in a rat model Cardiovasc Res, January 1, 2006; 69(1): 263 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Cominacini, M. Anselmi, U. Garbin, A. Fratta Pasini, C. Stranieri, M. Fusaro, C. Nava, P. Agostoni, D. Keta, P. Zardini, et al. Enhanced Plasma Levels of Oxidized Low-Density Lipoprotein Increase Circulating Nuclear Factor-Kappa B Activation in Patients With Unstable Angina J. Am. Coll. Cardiol., September 6, 2005; 46(5): 799 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Zmijewski, D. R. Moellering, C. L. Goffe, A. Landar, A. Ramachandran, and V. M. Darley-Usmar Oxidized LDL induces mitochondrially associated reactive oxygen/nitrogen species formation in endothelial cells Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H852 - H861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Inoue, Y. Arai, H. Kurihara, T. Kita, and T. Sawamura Overexpression of Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Induces Intramyocardial Vasculopathy in Apolipoprotein E-Null Mice Circ. Res., July 22, 2005; 97(2): 176 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Park, F. G. Adsit, and J. C. Boyington The 1.4 A Crystal Structure of the Human Oxidized Low Density Lipoprotein Receptor Lox-1 J. Biol. Chem., April 8, 2005; 280(14): 13593 - 13599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.L. Mehta, J. Chen, F. Yu, and D.Y. Li Aspirin inhibits ox-LDL-mediated LOX-1 expression and metalloproteinase-1 in human coronary endothelial cells Cardiovasc Res, November 1, 2004; 64(2): 243 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Li, N. Roumeliotis, T. Sawamura, and G. Renier C-Reactive Protein Enhances LOX-1 Expression in Human Aortic Endothelial Cells: Relevance of LOX-1 to C-Reactive Protein-Induced Endothelial Dysfunction Circ. Res., October 29, 2004; 95(9): 877 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Muscoli, I. Sacco, W. Alecce, E. Palma, R. Nistico, N. Costa, F. Clementi, D. Rotiroti, F. Romeo, D. Salvemini, et al. The Protective Effect of Superoxide Dismutase Mimetic M40401 on Balloon Injury-Related Neointima Formation: Role of the Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 J. Pharmacol. Exp. Ther., October 1, 2004; 311(1): 44 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. R. Rodriguez, S. Alam, and J. W. Lee Cytotoxicity of Oxidized Low-Density Lipoprotein in Cultured RPE Cells Is Dependent on the Formation of 7-Ketocholesterol Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2830 - 2837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Chade, M. D. Bentley, X. Zhu, M. Rodriguez-Porcel, S. Niemeyer, B. Amores-Arriaga, C. Napoli, E. L. Ritman, A. Lerman, and L. O. Lerman Antioxidant Intervention Prevents Renal Neovascularization in Hypercholesterolemic Pigs J. Am. Soc. Nephrol., July 1, 2004; 15(7): 1816 - 1825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Henriksen, M. Hitt, Z. Xing, J. Wang, C. Haslett, R. A. Riemersma, D. J. Webb, Y. V. Kotelevtsev, and J.-M. Sallenave Adenoviral Gene Delivery of Elafin and Secretory Leukocyte Protease Inhibitor Attenuates NF-{kappa}B-Dependent Inflammatory Responses of Human Endothelial Cells and Macrophages to Atherogenic Stimuli J. Immunol., April 1, 2004; 172(7): 4535 - 4544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Mango, F Clementi, P Borgiani, G B Forleo, M Federici, G Contino, E Giardina, L Garza, I E Fahdi, R Lauro, et al. Association of single nucleotide polymorphisms in the oxidised LDL receptor 1 (OLR1) gene in patients with acute myocardial infarction J. Med. Genet., December 1, 2003; 40(12): 933 - 936. [Full Text]
|
|
|
|
|
|
L. Cominacini, A. Fratta Pasini, U. Garbin, C. Nava, A. Davoli, M. Criscuoli, A. Crea, T. Sawamura, and V. Lo Cascio Nebivolol and its 4-keto derivative increase nitric oxide in endothelial cells by reducing its oxidative inactivation J. Am. Coll. Cardiol., November 19, 2003; 42(10): 1838 - 1844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Holven, H. Scholz, B. Halvorsen, P. Aukrust, L. Ose, and M. S. Nenseter Hyperhomocysteinemic Subjects Have Enhanced Expression of Lectin-Like Oxidized LDL Receptor-1 in Mononuclear Cells J. Nutr., November 1, 2003; 133(11): 3588 - 3591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Szmitko, C.-H. Wang, R. D. Weisel, G. A. Jeffries, T. J. Anderson, and S. Verma Biomarkers of Vascular Disease Linking Inflammation to Endothelial Activation: Part II Circulation, October 28, 2003; 108(17): 2041 - 2048. [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Bruun, A. S. Lihn, C. Verdich, S. B. Pedersen, S. Toubro, A. Astrup, and B. Richelsen Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E527 - E533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Chen, S. E. Reis, C. Kammerer, W. Y. Craig, S. E. LaPierre, E. L. Zimmer, D. M. McNamara, D. F. Pauly, B. Sharaf, R. Holubkov, et al. Genetic Variation in Lectin-Like Oxidized Low-Density Lipoprotein Receptor 1 (LOX1) Gene and the Risk of Coronary Artery Disease Circulation, July 1, 2003; 107(25): 3146 - 3151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Vielma, G. Krings, and M. F. Lopes-Virella Chlamydophila pneumoniae Induces ICAM-1 Expression in Human Aortic Endothelial Cells via Protein Kinase C-Dependent Activation of Nuclear Factor-{kappa}B Circ. Res., May 30, 2003; 92(10): 1130 - 1137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cominacini, A. Fratta Pasini, U. Garbin, A. Pastorino, A. Rigoni, C. Nava, A. Davoli, V. Lo Cascio, and T. Sawamura The platelet-endothelium interaction mediated by lectin-like oxidized low-density lipoprotein receptor-1 reduces the intracellular concentration of nitric oxide in endothelial cells J. Am. Coll. Cardiol., February 5, 2003; 41(3): 499 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Honjo, K. Nakamura, K. Yamashiro, J. Kiryu, H. Tanihara, L. M. McEvoy, Y. Honda, E. C. Butcher, T. Masaki, and T. Sawamura Lectin-like oxidized LDL receptor-1 is a cell-adhesion molecule involved in endotoxin-induced inflammation PNAS, February 4, 2003; 100(3): 1274 - 1279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Mehta and D. Li Identification, regulation and function of a novel lectin-like oxidized low-density lipoprotein receptor J. Am. Coll. Cardiol., May 1, 2002; 39(9): 1429 - 1435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Molavi, N. Rasouli, and J. L. Mehta Peroxisome Proliferator-Activated Receptor Ligands as Antiatherogenic Agents: Panacea or Another Pandora's Box? Journal of Cardiovascular Pharmacology and Therapeutics, March 1, 2002; 7(1): 1 - 8. [Abstract] [PDF]
|
|
|
|
|
|
T. Mikita, G. Porter, R. M. Lawn, and D. Shiffman Oxidized Low Density Lipoprotein Exposure Alters the Transcriptional Response of Macrophages to Inflammatory Stimulus J. Biol. Chem., November 30, 2001; 276(49): 45729 - 45739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Kevil, R. P. Patel, and D. C. Bullard Essential role of ICAM-1 in mediating monocyte adhesion to aortic endothelial cells Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1442 - C1447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. N. Wardle Nuclear factor {kappa}B for the nephrologist Nephrol. Dial. Transplant., September 1, 2001; 16(9): 1764 - 1768. [Full Text] [PDF]
|
|
|
|
|
|
H. Kataoka, N. Kume, S. Miyamoto, M. Minami, M. Morimoto, K. Hayashida, N. Hashimoto, and T. Kita Oxidized LDL Modulates Bax/Bcl-2 Through the Lectinlike Ox-LDL Receptor-1 in Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., June 1, 2001; 21(6): 955 - 960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Shen and A. Sevanian OxLDL induces macrophage {{gamma}}-GCS-HS protein expression: a role for oxLDL-associated lipid hydroperoxide in GSH synthesis J. Lipid Res., May 1, 2001; 42(5): 813 - 823. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Halvorsen, A. C. Staff, T. Henriksen, T. Sawamura, and T. Ranheim 8-iso-Prostaglandin F2{{alpha}} Increases Expression of LOX-1 in JAR Cells Hypertension, April 1, 2001; 37(4): 1184 - 1190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cominacini, A. Rigoni, A. F. Pasini, U. Garbin, A. Davoli, M. Campagnola, A. M. Pastorino, V. Lo Cascio, and T. Sawamura The Binding of Oxidized Low Density Lipoprotein (ox-LDL) to ox-LDL Receptor-1 Reduces the Intracellular Concentration of Nitric Oxide in Endothelial Cells through an Increased Production of Superoxide J. Biol. Chem., April 20, 2001; 276(17): 13750 - 13755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Denicola, C. Batthyany, E. Lissi, B. A. Freeman, H. Rubbo, and R. Radi Diffusion of Nitric Oxide into Low Density Lipoprotein J. Biol. Chem., January 4, 2002; 277(2): 932 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, D. Li, T. Saldeen, and J. L. Mehta Transforming Growth Factor-{beta}1 Modulates Oxidatively Modified LDL-Induced Expression of Adhesion Molecules: Role of LOX-1 Circ. Res., December 7, 2001; 89(12): 1155 - 1160. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
