|
J Biol Chem, Vol. 275, Issue 18, 13266-13274, May 5, 2000
Epithelial Sodium Channels Regulate Cystic Fibrosis Transmembrane
Conductance Regulator Chloride Channels in Xenopus
Oocytes*
Qinshi
Jiang §,
Jinqing
Li §,
Rachael
Dubroff ,
Yoon J.
Ahn §,
J. Kevin
Foskett¶,
John
Engelhardt , and
Thomas R.
Kleyman ¶**
From the Departments of Medicine and
¶ Physiology, University of Pennsylvania and Veterans
Affairs Medical Center, Philadelphia, Pennsylvania 19104-6144 and the
Department of Anatomy, University of Iowa,
Iowa City, Iowa 52242
The cystic fibrosis transmembrane conductance
regulator (CFTR), in addition to its well defined
Cl channel properties, regulates other ion
channels. CFTR inhibits epithelial Na+ channel (ENaC)
currents in many epithelial and nonepithelial cells. Because modulation
of net NaCl reabsorption has important implications in extracellular
fluid volume homeostasis and airway fluid volume and composition, we
investigated whether this regulation was reciprocal by examining
whether ENaC regulates CFTR. Co-expression of human (h) CFTR and mouse
(m)   ENaC in Xenopus oocytes resulted in a
significant, 3.7-fold increase in whole-cell hCFTR Cl
conductance compared with oocytes expressing hCFTR alone. The forskolin/3-isobutyl-1-methylxanthine-stimulated whole-cell conductance in hCFTR-mENaC co-injected oocytes was amiloride-insensitive, indicating an inhibition of mENaC following hCFTR activation, and it
was blocked by DPC (diphenylamine-2-carboxylic acid) and was DIDS
(4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid)-insensitive. Enhanced hCFTR Cl conductance was also observed when
either the - or -subunit of mENaC was co-expressed with hCFTR,
but this was not seen when CFTR was co-expressed with the -subunit
of mENaC. Single Cl channel analyses showed that both
CFTR Cl channel open probability and the number of CFTR
Cl channels detected per patch increased when hCFTR was
co-expressed with   mENaC. We conclude that in addition to
acting as a regulator of ENaC, CFTR activity is regulated by ENaC.
*
This work was supported in part by grants from the Cystic
Fibrosis Foundation (to J. K. F. and T. R. K.) and by National
Institutes of Health Grant DK56305.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
§
Recipients of postdoctoral fellowship awards from the Cystic
Fibrosis Foundation.
**
To whom correspondence should be addressed: Renal Division,
University of Pennsylvania, 700 CRB, 415 Curie Blvd., Philadelphia, PA
19104-6144. Tel.: 215-573-1848; Fax: 215-898-0189; E-mail: kleyman@mail.med.upenn.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
Y. Yaakov, E. Kerem, Y. Yahav, J. Rivlin, H. Blau, L. Bentur, M. Aviram, E. Picard, T. Bdolah-Abram, and M. Wilschanski
Reproducibility of Nasal Potential Difference Measurements in Cystic Fibrosis
Chest,
October 1, 2007;
132(4):
1219 - 1226.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Suaud, W. Yan, M. D. Carattino, A. Robay, T. R. Kleyman, and R. C. Rubenstein
Regulatory interactions of N1303K-CFTR and ENaC in Xenopus oocytes: evidence that chloride transport is not necessary for inhibition of ENaC
Am J Physiol Cell Physiol,
April 1, 2007;
292(4):
C1553 - C1561.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Suaud, W. Yan, and R. C. Rubenstein
Abnormal regulatory interactions of I148T-CFTR and the epithelial Na+ channel in Xenopus oocytes
Am J Physiol Cell Physiol,
January 1, 2007;
292(1):
C603 - C611.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
X. Su, Q. Li, K. Shrestha, E. Cormet-Boyaka, L. Chen, P. R. Smith, E. J. Sorscher, D. J. Benos, S. Matalon, and H.-L. Ji
Interregulation of Proton-gated Na+ Channel 3 and Cystic Fibrosis Transmembrane Conductance Regulator
J. Biol. Chem.,
December 1, 2006;
281(48):
36960 - 36968.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. M. Reddy and P. M. Quinton
Cytosolic potassium controls CFTR deactivation in human sweat duct
Am J Physiol Cell Physiol,
July 1, 2006;
291(1):
C122 - C129.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. B. Goldfarb, O. B. Kashlan, J. N. Watkins, L. Suaud, W. Yan, T. R. Kleyman, and R. C. Rubenstein
Differential effects of Hsc70 and Hsp70 on the intracellular trafficking and functional expression of epithelial sodium channels
PNAS,
April 11, 2006;
103(15):
5817 - 5822.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Bachhuber, J. Konig, T. Voelcker, B. Murle, R. Schreiber, and K. Kunzelmann
Cl- Interference with the Epithelial Na+ Channel ENaC
J. Biol. Chem.,
September 9, 2005;
280(36):
31587 - 31594.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Nagel, P. Barbry, H. Chabot, E. Brochiero, K. Hartung, and R. Grygorczyk
CFTR fails to inhibit the epithelial sodium channel ENaC expressed in Xenopus laevis oocytes
J. Physiol.,
May 1, 2005;
564(3):
671 - 682.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C.-T. Chang, M. Bens, E. Hummler, S. Boulkroun, L. Schild, J. Teulon, B. C. Rossier, and A. Vandewalle
Vasopressin-stimulated CFTR Cl- currents are increased in the renal collecting duct cells of a mouse model of Liddle's syndrome
J. Physiol.,
January 1, 2005;
562(1):
271 - 284.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
W. Yan, F. F. Samaha, M. Ramkumar, T. R. Kleyman, and R. C. Rubenstein
Cystic Fibrosis Transmembrane Conductance Regulator Differentially Regulates Human and Mouse Epithelial Sodium Channels in Xenopus Oocytes
J. Biol. Chem.,
May 28, 2004;
279(22):
23183 - 23192.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R.C. Boucher
New concepts of the pathogenesis of cystic fibrosis lung disease
Eur. Respir. J.,
January 1, 2004;
23(1):
146 - 158.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Suaud, M. Carattino, T. R. Kleyman, and R. C. Rubenstein
Genistein Improves Regulatory Interactions between G551D-Cystic Fibrosis Transmembrane Conductance Regulator and the Epithelial Sodium Channel in Xenopus Oocytes
J. Biol. Chem.,
December 20, 2002;
277(52):
50341 - 50347.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A.-A. Konstas, J.-P. Koch, S. J. Tucker, and C. Korbmacher
Cystic Fibrosis Transmembrane Conductance Regulator-dependent Up-regulation of Kir1.1 (ROMK) Renal K+ Channels by the Epithelial Sodium Channel
J. Biol. Chem.,
July 5, 2002;
277(28):
25377 - 25384.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Bagorda, L. Guerra, F. Di Sole, C. Hemle-Kolb, R. A. Cardone, T. Fanelli, S. J. Reshkin, S. M. Gisler, H. Murer, and V. Casavola
Reciprocal Protein Kinase A Regulatory Interactions between Cystic Fibrosis Transmembrane Conductance Regulator and Na+/H+ Exchanger Isoform 3 in a Renal Polarized Epithelial Cell Model
J. Biol. Chem.,
June 7, 2002;
277(24):
21480 - 21488.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik
Molecular Structure and Physiological Function of Chloride Channels
Physiol Rev,
April 1, 2002;
82(2):
503 - 568.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Suaud, J. Li, Q. Jiang, R. C. Rubenstein, and T. R. Kleyman
Genistein Restores Functional Interactions between Delta F508-CFTR and ENaC in Xenopus Oocytes
J. Biol. Chem.,
March 8, 2002;
277(11):
8928 - 8933.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. C. Broackes-Carter, N. Mouchel, D. Gill, S. Hyde, J. Bassett, and A. Harris
Temporal regulation of CFTR expression during ovine lung development: implications for CF gene therapy
Hum. Mol. Genet.,
January 1, 2002;
11(2):
125 - 131.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H.-L. Ji, M. L. Chalfant, B. Jovov, J. P. Lockhart, S. B. Parker, C. M. Fuller, B. A. Stanton, and D. J. Benos
The Cytosolic Termini of the beta - and gamma -ENaC Subunits Are Involved in the Functional Interactions between Cystic Fibrosis Transmembrane Conductance Regulator and Epithelial Sodium Channel
J. Biol. Chem.,
September 1, 2000;
275(36):
27947 - 27956.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|