Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guihard, G.
Right arrow Articles by Capiod, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guihard, G.
Right arrow Articles by Capiod, T.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 18, 13411-13414, May 5, 2000

Ca2+ Depletion and Inositol 1,4,5-Trisphosphate-evoked Activation of Ca2+ Entry in Single Guinea Pig Hepatocytes*

Gilles GuihardDagger , Jacques Noel§, and Thierry Capiod

From INSERM U442, Université Paris-Sud, Bât. 443, 91405 Orsay, France

Store-operated Ca2+ entry was investigated by monitoring the Ca2+-dependent K+ permeability in voltage-clamped guinea pig hepatocytes. In physiological conditions, intracellular Ca2+ stores are discharged following agonist stimulation, but depletion of this stores can be achieved using Ca2+-Mg2+-ATPase inhibitors such as 2,5-di(tert-butyl)-1,4-benzohydroquinone and thapsigargin. The effect of internal Ca2+ store depletion on Ca2+ influx was tested in single cells using inositol 1,4,5-trisphosphate (InsP3) release from caged InsP3 after treatment of the cells with 2,5-di(tert-butyl)-1,4-benzohydroquinone or thapsigargin in Ca2+-free solutions. We show that the photolytic release of 1-D-myo-inositol 1,4-bisphosphate 5-phosphorothioate, a stable analog of InsP3, and Ca2+ store depletion have additive effects to activate a high level of Ca2+ entry in single guinea pig hepatocytes. These results suggest that there is a direct functional interaction between InsP3 receptors and Ca2+ channels in the plasma membrane, although the nature of these Ca2+ channels in hepatocytes is unclear.


* This work was supported by Association pour la Recherche sur le Cancer Grant 5241 (to T. C.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported by a Singer-Polignac sponsorship. Present address: INSERM U533, Hôtel-Dieu, 44000 Nantes, France.

§ Present address: EA 2674, Université de Nice-Sophia Antipolis, Faculté des sciences, Parc Valrose, 06108 Nice, France.

To whom correspondence and reprint requests should be addressed. Tel.: 33-169156865; Fax: 33-169155893; E-mail: thierry.capiod@ibaic.u-psud.fr.


Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Physiol. Rev.Home page
M. C. Ashby and A. V. Tepikin
Polarized Calcium and Calmodulin Signaling in Secretory Epithelia
Physiol Rev, July 1, 2002; 82(3): 701 - 734.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement