JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rader, C.
Right arrow Articles by Barbas, C. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rader, C.
Right arrow Articles by Barbas, C. F., III
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 18, 13668-13676, May 5, 2000

The Rabbit Antibody Repertoire as a Novel Source for the Generation of Therapeutic Human Antibodies*

Christoph RaderDagger §, Gerd Ritter, Sheila NathanDagger ||, Marikka EliaDagger , Ivan Gout**, Achim A. Jungbluth, Leonard S. Cohen, Sydney Welt, Lloyd J. Old, and Carlos F. Barbas IIIDagger Dagger Dagger

From Dagger  The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, the  Ludwig Institute for Cancer Research, New York Branch, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, and the ** Ludwig Institute for Cancer Research, London Branch at University College, London School of Medicine, W1P 8BT London, United Kingdom

The rabbit antibody repertoire, which in the form of polyclonal antibodies has been used in diagnostic applications for decades, would be an attractive source for the generation of therapeutic human antibodies. The humanization of rabbit antibodies, however, has not been reported. Here we use phage display technology to select and humanize antibodies from rabbits that were immunized with human A33 antigen which is a target antigen for the immunotherapy of colon cancer. We first selected rabbit antibodies that bind to a cell surface epitope of human A33 antigen with an affinity in the 1 nM range. For rabbit antibody humanization, we then used a selection strategy that combines grafting of the complementarity determining regions with framework fine tuning. The resulting humanized antibodies were found to retain both high specificity and affinity for human A33 antigen.

* This work was supported in part by The Skaggs Institute for Chemical Biology.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF 245498-AF 245503.

§ Supported by postdoctoral fellowships from the Swiss National Science Foundation and the Krebsliga des Kantons Zürich.

|| Current address: CGAT, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia.

Dagger Dagger To whom correspondence should be addressed: 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Tel.: 858-784-9098; Fax: 858-784-2583; E-mail: carlos@scripps.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Protein Eng Des SelHome page
U. Petrausch, J. Dernedde, V. Coelho, H. Panjideh, D. Frey, H. Fuchs, E. Thiel, and P. M. Deckert
A33scFv Green fluorescent protein, a recombinant single-chain fusion protein for tumor targeting
Protein Eng. Des. Sel., December 1, 2007; 20(12): 583 - 590.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Bonasio, C. V. Carman, E. Kim, P. T. Sage, K. R. Love, T. R. Mempel, T. A. Springer, and U. H. von Andrian
Specific and covalent labeling of a membrane protein with organic fluorochromes and quantum dots
PNAS, September 11, 2007; 104(37): 14753 - 14758.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
A. R. Hunt, S. Frederickson, C. Hinkel, K. S. Bowdish, and J. T. Roehrig
A humanized murine monoclonal antibody protects mice either before or after challenge with virulent Venezuelan equine encephalomyelitis virus
J. Gen. Virol., September 1, 2006; 87(9): 2467 - 2476.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Jendreyko, M. Popkov, C. Rader, and C. F. Barbas III
Phenotypic knockout of VEGF-R2 and Tie-2 with an intradiabody reduces tumor growth and angiogenesis in vivo
PNAS, June 7, 2005; 102(23): 8293 - 8298.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
X.-B. Wang, B. Zhou, C.-C. Yin, Q. Lin, and H.-L. Huang
A New Approach for Rapidly Reshaping Single-Chain Antibody In Vitro by Combining DNA Shuffling with Ribosome Display
J. Biochem., July 1, 2004; 136(1): 19 - 28.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Jendreyko, M. Popkov, R. R. Beerli, J. Chung, D. B. McGavern, C. Rader, and C. F. Barbas III
Intradiabodies, Bispecific, Tetravalent Antibodies for the Simultaneous Functional Knockout of Two Cell Surface Receptors
J. Biol. Chem., November 28, 2003; 278(48): 47812 - 47819.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Goncalves, F. Silva, A. Freitas-Vieira, M. Santa-Marta, R. Malho, X. Yang, D. Gabuzda, and C. Barbas III
Functional Neutralization of HIV-1 Vif Protein by Intracellular Immunization Inhibits Reverse Transcription and Viral Replication
J. Biol. Chem., August 23, 2002; 277(35): 32036 - 32045.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. Ritter, L. S. Cohen, C. Williams Jr., E. C. Richards, L. J. Old, and S. Welt
Serological Analysis of Human Anti-Human Antibody Responses in Colon Cancer Patients Treated with Repeated Doses of Humanized Monoclonal Antibody A33
Cancer Res., September 1, 2001; 61(18): 6851 - 6859.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Steinberger, J. K. Sutton, C. Rader, M. Elia, and C. F. Barbas III
Generation and Characterization of a Recombinant Human CCR5-specific Antibody. A PHAGE DISPLAY APPROACH FOR RABBIT ANTIBODY HUMANIZATION
J. Biol. Chem., November 10, 2000; 275(46): 36073 - 36078.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.