JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eckmann, L.
Right arrow Articles by Kagnoff, M. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eckmann, L.
Right arrow Articles by Kagnoff, M. F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 19, 14084-14094, May 12, 2000

Analysis by High Density cDNA Arrays of Altered Gene Expression in Human Intestinal Epithelial Cells in Response to Infection with the Invasive Enteric Bacteria Salmonella*

Lars EckmannDagger , Jennifer R. Smith, Michael P. Housley, Michael B. Dwinell, and Martin F. Kagnoff

From the Department of Medicine, University of California, San Diego, La Jolla, California 92093

Many clinically important enteric pathogens initiate disease by invading and passing through the intestinal epithelium, a process accompanied by increased epithelial expression of proinflammatory cytokines. To further define the role intestinal epithelial cells play in initiating and modulating the host response to infection with invasive bacteria, hybrid selection on high density cDNA arrays was used to characterize the mRNA expression profile of ~4,300 genes in human intestinal epithelial cells after infection with the prototypic invasive bacteria, Salmonella. Selected findings were further evaluated by reverse transcription-polymerase chain reaction, Northern blot analysis, and protein assays. Epithelial infection with Salmonella significantly up-regulated mRNA expression of a relatively small fraction of all genes tested. Of these, several cytokines (granulocyte colony-stimulating factor, inhibin A, Epstein-Barr virus-induced gene 3, interleukin-8, macrophage inflammatory protein-2alpha ), kinases (TKT, Eck, HEK), transcription factors (interferon regulatory factor-1), and HLA class I were the most prominent. Furthermore, the transcription factor NF-kappa B is shown to be important for inducible mRNA expression for a broad group of genes tested. These findings expand the repertoire of known epithelial cell responses to infection with an invasive enteric pathogen. The results also show that evaluation of mRNA expression profiles by cDNA array analysis is a powerful approach to characterizing and understanding host-pathogen interactions.

* This work was supported by National Institutes of Health Grant DK35108 and a research grant from the Crohn's and Colitis Foundation of America.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: University of California, San Diego, Department of Medicine 0623D, 9500 Gilman Dr., La Jolla, CA 92093-0623. Tel.: 858-534-0683; Fax: 858-534-5691; E-mail: leckmann@ucsd.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
H. Echchannaoui, M. Bianchi, D. Baud, M. Bobst, J.-C. Stehle, and D. Nardelli-Haefliger
Intravaginal Immunization of Mice with Recombinant Salmonella enterica Serovar Typhimurium Expressing Human Papillomavirus Type 16 Antigens as a Potential Route of Vaccination against Cervical Cancer
Infect. Immun., May 1, 2008; 76(5): 1940 - 1951.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
W. Rodenburg, I. M. J. Bovee-Oudenhoven, E. Kramer, R. van der Meer, and J. Keijer
Gene expression response of the rat small intestine following oral Salmonella infection
Physiol Genomics, July 18, 2007; 30(2): 123 - 133.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
C. M. T. Dvorak, G. N. Hirsch, K. A. Hyland, J. A. Hendrickson, B. S. Thompson, M. S. Rutherford, and M. P. Murtaugh
Genomic dissection of mucosal immunobiology in the porcine small intestine
Physiol Genomics, December 13, 2006; 28(1): 5 - 14.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. C. Yang, H. Ogawa, M. B. Dwinell, D. F. McCole, L. Eckmann, and M. F. Kagnoff
Chemokine receptor CCR6 transduces signals that activate p130Cas and alter cAMP-stimulated ion transport in human intestinal epithelial cells
Am J Physiol Cell Physiol, February 1, 2005; 288(2): C321 - C328.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. UNNIKRISHNAN and B. A. BURLEIGH
Inhibition of host connective tissue growth factor expression: a novel Trypanosoma cruzi-mediated response
FASEB J, November 1, 2004; 18(14): 1625 - 1635.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
F.-C. Huang, A. Werne, Q. Li, E. E. Galyov, W. A. Walker, and B. J. Cherayil
Cooperative Interactions between Flagellin and SopE2 in the Epithelial Interleukin-8 Response to Salmonella enterica Serovar Typhimurium Infection
Infect. Immun., September 1, 2004; 72(9): 5052 - 5062.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
N. R. Stokes, X. Zhou, S. J. Meltzer, and J. B. Kaper
Transcriptional Responses of Intestinal Epithelial Cells to Infection with Vibrio cholerae
Infect. Immun., July 1, 2004; 72(7): 4240 - 4248.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S.-L. Yu, H.-W. Chen, P.-C. Yang, K. Peck, M.-H. Tsai, J. J. W. Chen, and F.-Y. Lin
Differential Gene Expression in Gram-negative and Gram-positive Sepsis
Am. J. Respir. Crit. Care Med., May 15, 2004; 169(10): 1135 - 1143.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. B. Dwinell, H. Ogawa, K. E. Barrett, and M. F. Kagnoff
SDF-1/CXCL12 regulates cAMP production and ion transport in intestinal epithelial cells via CXCR4
Am J Physiol Gastrointest Liver Physiol, May 1, 2004; 286(5): G844 - G850.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. G. Kim, S. J. Lee, and M. F. Kagnoff
Nod1 Is an Essential Signal Transducer in Intestinal Epithelial Cells Infected with Bacteria That Avoid Recognition by Toll-Like Receptors
Infect. Immun., March 1, 2004; 72(3): 1487 - 1495.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
S. C. M. Tsoi, J. M. Cale, I. M. Bird, and H. H. Kay
cDNA Microarray Analysis of the Gene Expression Profiles in Human Placenta: Up-Regulation of the Transcript Encoding Muscle Subunit of Glycogen Phosphorylase in Preeclampsia
Reproductive Sciences, December 1, 2003; 10(8): 496 - 502.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. J. Egan, A. de Lecea, E. D. Lehrman, G. M. Myhre, L. Eckmann, and M. F. Kagnoff
Nuclear factor-{kappa}B activation promotes restitution of wounded intestinal epithelial monolayers
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1028 - C1035.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Zeng, A. Q. Carlson, Y. Guo, Y. Yu, L. S. Collier-Hyams, J. L. Madara, A. T. Gewirtz, and A. S. Neish
Flagellin Is the Major Proinflammatory Determinant of Enteropathogenic Salmonella
J. Immunol., October 1, 2003; 171(7): 3668 - 3674.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Pedron, C. Thibault, and P. J. Sansonetti
The Invasive Phenotype of Shigella flexneri Directs a Distinct Gene Expression Pattern in the Human Intestinal Epithelial Cell Line Caco-2
J. Biol. Chem., September 5, 2003; 278(36): 33878 - 33886.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Zhang, L. G. Adams, J. Nunes, S. Khare, R. M. Tsolis, and A. J. Baumler
Secreted Effector Proteins of Salmonella enterica Serotype Typhimurium Elicit Host-Specific Chemokine Profiles in Animal Models of Typhoid Fever and Enterocolitis
Infect. Immun., August 1, 2003; 71(8): 4795 - 4803.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. E. Girardin, I. G. Boneca, L. A. M. Carneiro, A. Antignac, M. Jehanno, J. Viala, K. Tedin, M.-K. Taha, A. Labigne, U. Zathringer, et al.
Nod1 Detects a Unique Muropeptide from Gram-Negative Bacterial Peptidoglycan
Science, June 6, 2003; 300(5625): 1584 - 1587.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
J. R. Kimbell and M. J. McFall-Ngai
The Squid-Vibrio Symbioses: From Demes to Genes
Integr. Comp. Biol., April 1, 2003; 43(2): 254 - 260.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Hauf and T. Chakraborty
Suppression of NF-{kappa}B Activation and Proinflammatory Cytokine Expression by Shiga Toxin-Producing Escherichia coli
J. Immunol., February 15, 2003; 170(4): 2074 - 2082.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Zhang, R. A. Kingsley, R. L. Santos, H. Andrews-Polymenis, M. Raffatellu, J. Figueiredo, J. Nunes, R. M. Tsolis, L. G. Adams, and A. J. Baumler
Molecular Pathogenesis of Salmonella enterica Serotype Typhimurium-Induced Diarrhea
Infect. Immun., January 1, 2003; 71(1): 1 - 12.
[Full Text] [PDF]

Home page
 J. Bacteriol.Home page
R. McNab, S. K. Ford, A. El-Sabaeny, B. Barbieri, G. S. Cook, and R. J. Lamont
LuxS-Based Signaling in Streptococcus gordonii: Autoinducer 2 Controls Carbohydrate Metabolism and Biofilm Formation with Porphyromonas gingivalis
J. Bacteriol., January 1, 2003; 185(1): 274 - 284.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. A. Vallance, W. Deng, M. De Grado, C. Chan, K. Jacobson, and B. B. Finlay
Modulation of Inducible Nitric Oxide Synthase Expression by the Attaching and Effacing Bacterial Pathogen Citrobacter rodentium in Infected Mice
Infect. Immun., November 1, 2002; 70(11): 6424 - 6435.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. Kirby, F. M. Menzies, M. R. Cookson, K. Bushby, and P. J. Shaw
Differential gene expression in a cell culture model of SOD1-related familial motor neurone disease
Hum. Mol. Genet., August 15, 2002; 11(17): 2061 - 2075.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Sauvonnet, B. Pradet-Balade, J. A. Garcia-Sanz, and G. R. Cornelis
Regulation of mRNA Expression in Macrophages after Yersinia enterocolitica Infection. ROLE OF DIFFERENT Yop EFFECTORS
J. Biol. Chem., July 5, 2002; 277(28): 25133 - 25142.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
R. L. Santos, S. Zhang, R. M. Tsolis, A. J. Baumler, and L. G. Adams
Morphologic and Molecular Characterization of Salmonella typhimurium Infection in Neonatal Calves
Vet. Pathol., March 1, 2002; 39(2): 200 - 215.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. V. de Avalos, I. J. Blader, M. Fisher, J. C. Boothroyd, and B. A. Burleigh
Immediate/Early Response to Trypanosoma cruzi Infection Involves Minimal Modulation of Host Cell Transcription
J. Biol. Chem., January 4, 2002; 277(1): 639 - 644.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
J. M. Cox, C. L. Clayton, T. Tomita, D. M. Wallace, P. A. Robinson, and J. E. Crabtree
cDNA Array Analysis of cag Pathogenicity Island-Associated Helicobacter pylori Epithelial Cell Response Genes
Infect. Immun., November 1, 2001; 69(11): 6970 - 6980.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Edde, R. B. Hipolito, F. F. Y. Hwang, D. R. Headon, R. A. Shalwitz, and M. P. Sherman
Lactoferrin protects neonatal rats from gut-related systemic infection
Am J Physiol Gastrointest Liver Physiol, November 1, 2001; 281(5): G1140 - G1150.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. Zhao, R. A. Bowden, S. A. Stavchansky, and P. D. Bowman
Human endothelial cell response to gram-negative lipopolysaccharide assessed with cDNA microarrays
Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1587 - C1595.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. de Grado, C. M. Rosenberger, A. Gauthier, B. A. Vallance, and B. B. Finlay
Enteropathogenic Escherichia coli Infection Induces Expression of the Early Growth Response Factor by Activating Mitogen-Activated Protein Kinase Cascades in Epithelial Cells
Infect. Immun., October 1, 2001; 69(10): 6217 - 6224.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
W. O. Chung, Y. Park, R. J. Lamont, R. McNab, B. Barbieri, and D. R. Demuth
Signaling System in Porphyromonas gingivalis Based on a LuxS Protein
J. Bacteriol., July 1, 2001; 183(13): 3903 - 3909.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
S. Lucchini, A. Thompson, and J. C. D. Hinton
Microarrays for microbiologists
Microbiology, June 1, 2001; 147(6): 1403 - 1414.
[Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. N. Young, M. B. Amin, C. S. Moreno, S. D. Lim, C. Cohen, J. A. Petros, F. F. Marshall, and A. S. Neish
Expression Profiling of Renal Epithelial Neoplasms : A Method for Tumor Classification and Discovery of Diagnostic Molecular Markers
Am. J. Pathol., May 1, 2001; 158(5): 1639 - 1651.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
M. R. SABAN, H. HELLMICH, N.-B. NGUYEN, J. WINSTON, T. G. HAMMOND, and R. SABAN
Time course of LPS-induced gene expression in a mouse model of genitourinary inflammation
Physiol Genomics, April 2, 2001; 5(3): 147 - 160.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Izadpanah, M. B. Dwinell, L. Eckmann, N. M. Varki, and M. F. Kagnoff
Regulated MIP-3{alpha}/CCL20 production by human intestinal epithelium: mechanism for modulating mucosal immunity
Am J Physiol Gastrointest Liver Physiol, April 1, 2001; 280(4): G710 - G719.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. K. Coombes and J. B. Mahony
cDNA Array Analysis of Altered Gene Expression in Human Endothelial Cells in Response to Chlamydia pneumoniae Infection
Infect. Immun., March 1, 2001; 69(3): 1420 - 1427.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Rappuoli
Pushing the limits of cellular microbiology: Microarrays to study bacteria-host cell intimate contacts
PNAS, November 22, 2000; (2000) 10505497.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. E. Belcher, J. Drenkow, B. Kehoe, T. R. Gingeras, N. McNamara, H. Lemjabbar, C. Basbaum, and D. A. Relman
The transcriptional responses of respiratory epithelial cells to Bordetella pertussis reveal host defensive and pathogen counter-defensive strategies
PNAS, November 16, 2000; (2000) 230262797.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Rappuoli
Pushing the limits of cellular microbiology: Microarrays to study bacteria-host cell intimate contacts
PNAS, December 5, 2000; 97(25): 13467 - 13469.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. E. Belcher, J. Drenkow, B. Kehoe, T. R. Gingeras, N. McNamara, H. Lemjabbar, C. Basbaum, and D. A. Relman
From the Cover: The transcriptional responses of respiratory epithelial cells to Bordetella pertussis reveal host defensive and pathogen counter-defensive strategies
PNAS, December 5, 2000; 97(25): 13847 - 13852.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.