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J Biol Chem, Vol. 275, Issue 19, 14354-14359, May 12, 2000
From the Departments of Studies of the structural organization of
calcium/ calmodulin-dependent protein kinase II
Three-dimensional Reconstructions of
Calcium/Calmodulin-dependent (CaM) Kinase II
and Truncated CaM
Kinase II Reveal a Unique Organization for Its Structural Core
and Functional Domains*
,
Pathology and Laboratory
Medicine and § Neurobiology and Anatomy, University of
Texas Health Science Center, Houston, Texas 77030
(CaM
KII) and truncated CaM KII by three-dimensional electron
microscopy and protein engineering show that the structures consist of
12 subunits that are organized in two stacked hexameric rings with 622 symmetry. The body of CaM KII is gear-shaped, consisting of six
slanted flanges, and has six foot-like processes attached by narrow
appendages to both ends of the flanges. Truncated CaM KII that lacks
functional domains has a structure that is very similar to the body of
CaM KII. Thus, the functional domains reside in the foot-like
processes, and the association domain comprises the gear-shaped core.
The ribbon diagram of the bilobate structure of CaM KI fits nicely in
the envelope of the foot-like component and indicates that the crevice
between the two lobes comprising the functional domains is near the
middle portion of the foot. The clustering of the functional domains
provides a favorable arrangement for the autophosphorylation reaction,
and the unusual arrangement of the catalytic domain on extended tethers
appears to be significant for the remarkable functional diversity of
CaM KII in cellular regulation.
*
This work was supported in part by United States Public
Health Service Grants NS 26086 (to N. M. W. and J. K. S.). A
portion of this study is part of the dissertation presented by A. H.
to the University of Texas Graduate School of Biomedical Sciences at
Houston in partial fulfillment of the Ph.D. degree.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
713-500-5345; Fax: 713-500-0730; E-mail:
James.K.Stoops@uth.tmc.edu.
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