| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 19, 14401-14407, May 12, 2000
From the We have cloned a new P2X ligand-gated ion channel
receptor from embryonic chick skeletal muscle, which is tentatively
named as chick P2X8 (cP2X8) receptor. The
cloned cDNA encodes a protein with 402 amino acids.
Electrophysiological study of the recombinant cP2X8
receptor expressed in Xenopus oocytes showed that 10 µM ATP induced a fast inward current followed by rapid
and long lasting desensitization in medium containing 1.8 mM Ca2+. In medium with 0.3 mM
Ca2+ ATP induced a bi-phasic response as follows: a slower
inward current succeeded the initial fast one. 2-Methylthio-ATP,
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF205066.
Molecular Cloning and Characterization of a Novel ATP P2X
Receptor Subtype from Embryonic Chick Skeletal Muscle*
§¶,
Autonomic Neuroscience Institute, Royal Free
and University College Hospital School of Medicine, Rowland Hill
Street, London NW3 2PF and § Wellcome Laboratory for
Molecular Pharmacology and Department of Pharmacology, University
College London, Gower Street, London WC1E 6BT, United Kingdom
,
-methylene-ATP, and adenosine
5'-O-(thio)triphosphate were potent agonists, whereas ADP
was a very weak agonist. ATP-induced currents were blocked by 100 µM suramin and pyridoxal phosphate
6-azophenyl-2',4'-disulfonic acid. Northern blot analysis and reverse
transcription-polymerase chain reaction showed that cP2X8
RNA transcripts were mainly expressed in skeletal muscle, brain, and
heart of Day 10 chick embryos. A moderate level of expression was also
detected in gizzard and retina. Whole mount in situ
hybridization showed that cP2X8 RNA transcripts were
expressed mainly in neurotube, notochord, and stomach in Day 3 embryos.
In Day 4 and Day 6 embryos, the cP2X8 RNA transcripts were
highly expressed in the myotome and premuscle mass. The physiological
role of this receptor in the establishment of the skeletal muscle
innervation will be studied.
*
This work was supported by grants from the Wellcome Trust
(to G. B. and R. S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. D. Sacks, G. M. Cann, W. Nikovits Jr, S. Conlon, N. R. Espinoza, and F. E. Stockdale Regulation of myosin expression during myotome formation Development, August 1, 2003; 130(15): 3391 - 3402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Bo, L.-H. Jiang, H. L. Wilson, M. Kim, G. Burnstock, A. Surprenant, and R. A. North Pharmacological and Biophysical Properties of the Human P2X5 Receptor Mol. Pharmacol., June 1, 2003; 63(6): 1407 - 1416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. North Molecular Physiology of P2X Receptors Physiol Rev, October 1, 2002; 82(4): 1013 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-H. Jo and L. W. Role Coordinate Release of ATP and GABA at In Vitro Synapses of Lateral Hypothalamic Neurons J. Neurosci., June 15, 2002; 22(12): 4794 - 4804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Y. Choi, M. L. S. Man, K. K. Y. Ling, N. Y. Ip, J. Simon, E. A. Barnard, and K. W. K. Tsim Expression of the P2Y1 Nucleotide Receptor in Chick Muscle: Its Functional Role in the Regulation of Acetylcholinesterase and Acetylcholine Receptor J. Neurosci., December 1, 2001; 21(23): 9224 - 9234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Jensik, D. Holbird, M. W. Collard, and T. C. Cox Cloning and characterization of a functional P2X receptor from larval bullfrog skin Am J Physiol Cell Physiol, September 1, 2001; 281(3): C954 - C962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. W. Inscho P2 receptors in regulation of renal microvascular function Am J Physiol Renal Physiol, June 1, 2001; 280(6): F927 - F944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Khakh, G. Burnstock, C. Kennedy, B. F. King, R. A. North, P. Seguela, M. Voigt, and P. P. A. Humphrey International Union of Pharmacology. XXIV. Current Status of the Nomenclature and Properties of P2X Receptors and Their Subunits Pharmacol. Rev., March 1, 2001; 53(1): 107 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jankowski, J. Hagemann, M. Tepel, M. van der Giet, N. Stephan, L. Henning, H. Gouni-Berthold, A. Sachinidis, W. Zidek, and H. Schluter Dinucleotides as Growth-promoting Extracellular Mediators. PRESENCE OF DINUCLEOSIDE DIPHOSPHATES Ap2A, Ap2G, AND Gp2G IN RELEASABLE GRANULES OF PLATELETS J. Biol. Chem., March 16, 2001; 276(12): 8904 - 8909. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
