| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 19, 14482-14493, May 12, 2000
From the The tumor necrosis factor-
An Ordered Array of Cold Shock Domain Repressor Elements across
Tumor Necrosis Factor-responsive Elements of the
Granulocyte-Macrophage Colony-stimulating Factor Promoter*
§,,,, and Division of Human Immunology, Hanson Centre
for Cancer Research, Institute of Medical and Veterinary Science, Frome
Road, Adelaide, South Australia, 5000, Australia and the ¶ John
Curtin School of Medical Research, Australian National University,
Canberra, ACT 2000, Australia
-responsive region
of the human granulocyte-macrophage colony-stimulating factor (GM-CSF)
promoter (
114 to 31) encompasses binding sites for NF-
B, CBF,
AP-1, ETS, and NFAT families of transcription factors. We show both here and previously that mutation of any one of these binding sites
greatly reduces tumor necrosis factor- induction of the GM-CSF
promoter. Interspersed between these elements are sequences that when
mutated lead to an increase in GM-CSF promoter activity. We have
previously shown that two of these repressor elements bind proteins
known as cold shock domain (CSD) factors and that overexpression of CSD
proteins leads to repression of GM-CSF promoter activity in
fibroblasts. CSD proteins are single strand DNA- and RNA-binding
proteins that contact 5'-CCTG-3' sequences in the GM-CSF repressor
elements. We show here that two newly identified repressor sequences in
the proximal promoter can also bind CSD proteins. We have characterized
the CSD-containing protein complexes that bind to the GM-CSF promoter
and identified a novel protein related to mitochondrial single strand
binding protein that forms part of one of these complexes. The four
CSD-binding sites on the promoter occur in pairs on opposite strands of
the DNA and appear to form an ordered array of binding elements. A
similar ordered array of CSD sites are present in the promoters of the granulocyte colony-stimulating factor and interleukin-3 genes, implying
a common mechanism for negative regulation of these myeloid growth factors.
*
This work was supported by a National Health and Medical
Research Council, Australia, Project Grant (to M. F. S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Berghella, L. De Angelis, T. De Buysscher, A. Mortazavi, S. Biressi, S. V. Forcales, D. Sirabella, G. Cossu, and B. J. Wold A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle Genes & Dev., August 1, 2008; 22(15): 2125 - 2138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R.C. van Roeyen, F. Eitner, S. Martinkus, S. R. Thieltges, T. Ostendorf, D. Bokemeyer, B. Luscher, J. M. Luscher-Firzlaff, J. Floege, and P. R. Mertens Y-Box Protein 1 Mediates PDGF-B Effects in Mesangioproliferative Glomerular Disease J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2985 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zhang, X. Liu, J. G. Cogan, M. D. Fuerst, J. A. Polikandriotis, R. J. Kelm Jr., and A. R. Strauch YB-1 Coordinates Vascular Smooth Muscle {alpha}-Actin Gene Activation by Transforming Growth Factor {beta}1 and Thrombin during Differentiation of Human Pulmonary Myofibroblasts Mol. Biol. Cell, October 1, 2005; 16(10): 4931 - 4940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Coles, P. Diamond, L. Lambrusco, J. Hunter, J. Burrows, M. A. Vadas, and G. J. Goodall A novel mechanism of repression of the vascular endothelial growth factor promoter, by single strand DNA binding cold shock domain (Y-box) proteins in normoxic fibroblasts Nucleic Acids Res., November 15, 2002; 30(22): 4845 - 4854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bozinovski, J. E. Jones, R. Vlahos, J. A. Hamilton, and G. P. Anderson Granulocyte/Macrophage-Colony-stimulating Factor (GM-CSF) Regulates Lung Innate Immunity to Lipopolysaccharide through Akt/Erk Activation of NFkappa B and AP-1 in Vivo J. Biol. Chem., November 1, 2002; 277(45): 42808 - 42814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. V. Subramanian, R. J. Kelm Jr., J. A. Polikandriotis, C. G. Orosz, and A. R. Strauch Reprogramming of vascular smooth muscle {alpha}-actin gene expression as an early indicator of dysfunctional remodeling following heart transplant Cardiovasc Res, June 1, 2002; 54(3): 539 - 548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E. Capowski, S. Esnault, S. Bhattacharya, and J. S. Malter Y Box-Binding Factor Promotes Eosinophil Survival by Stabilizing Granulocyte-Macrophage Colony-Stimulating Factor mRNA J. Immunol., November 15, 2001; 167(10): 5970 - 5976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Shannon, L. S. Coles, J. Attema, and P. Diamond The role of architectural transcription factors in cytokine gene transcription J. Leukoc. Biol., January 1, 2001; 69(1): 21 - 32. [Abstract] [Full Text]
|
|
|
|
|
|
P. Diamond, M. F. Shannon, M. A. Vadas, and L. S. Coles Cold Shock Domain Factors Activate the Granulocyte-Macrophage Colony-stimulating Factor Promoter in Stimulated Jurkat T Cells J. Biol. Chem., March 9, 2001; 276(11): 7943 - 7951. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
