JBC Oz Biosciences

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goetzl, E. J.
Right arrow Articles by Karliner, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goetzl, E. J.
Right arrow Articles by Karliner, J. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 19, 14573-14578, May 12, 2000

Gelsolin Binding and Cellular Presentation of Lysophosphatidic Acid*

Edward J. GoetzlDagger , Hsinyu Lee, Toshifumi Azuma§, Thomas P. Stossel§, Christoph W. Turck, and Joel S. Karliner

From the Departments of Medicine and Microbiology-Immunology and  Veterans Affairs Medical Center, University of California, San Francisco, California 94143-0711 and § Department of Medicine, Hematology Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115

Lysophosphatidic acid (LPA) in biological fluids binds to serum albumin and other proteins that enhance its effects on cellular functions. The actin-severing protein gelsolin binds LPA with an affinity (Kd = 6 nM) similar to that of the G protein-coupled LPA receptors encoded by endothelial differentiation genes 2, 4, and 7 (Edg-2, -4, and -7 receptors) and greater than that of serum albumin (Kd = 360 nM). At concentrations of 10% or less of that in plasma, which are observed in fluids of injured tissues, purified and recombinant gelsolin augment LPA stimulation of nuclear signals and protein synthesis in rat cardiac myocytes (RCMs) that express Edg-2 and -4 receptors. At concentrations of 20% or more of that in plasma, gelsolin suppresses LPA stimulation of RCMs. The lack of effect of gelsolin on RCM responses to monoclonal anti-Edg-4 receptor antibody plus a phorbol ester without LPA attests to its specificity for LPA delivery and the absence of post-receptor effects. Inhibition of gelsolin binding and cellular delivery of LPA by L-alpha -phosphatidylinositol-4,5-bisphosphate (PIP2) and peptides constituting the two PIP2 binding domains of gelsolin suggests competition between LPA and PIP2 for the same sites. Thus, delivery of LPA to RCMs is affinity-coupled to Edg receptors by gelsolin in a PIP2-regulated process.

* This work was supported in part by National Institutes of Health Grants HL31809 (to E. J. G.) and HL54253 (to T. P. S.) and a Merit Review grant from the Department of Veterans Affairs Research Service (to J. S. K.). These data were presented in part at the 1999 annual meeting of the American Society for Biochemistry and Molecular Biology (41).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: University of California, UB8B, Box 0711, 533 Parnassus, San Francisco, CA 94143-0711. Tel.: 415-476-5339; Fax: 415-476-6915; E-mail: egoetzl@itsa.ucsf.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
R. Bucki, F. J. Byfield, A. Kulakowska, M. E. McCormick, W. Drozdowski, Z. Namiot, T. Hartung, and P. A. Janmey
Extracellular Gelsolin Binds Lipoteichoic Acid and Modulates Cellular Response to Proinflammatory Bacterial Wall Components
J. Immunol., October 1, 2008; 181(7): 4936 - 4944.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
S.-U. Chen, C.-H. Chou, H. Lee, C.-H. Ho, C.-W. Lin, and Y.-S. Yang
Lysophosphatidic Acid Up-Regulates Expression of Interleukin-8 and -6 in Granulosa-Lutein Cells through Its Receptors and Nuclear Factor-{kappa}B Dependent Pathways: Implications for Angiogenesis of Corpus Luteum and Ovarian Hyperstimulation Syndrome
J. Clin. Endocrinol. Metab., March 1, 2008; 93(3): 935 - 943.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Zhang, A. Bialkowska, R. Rusovici, S. Chanchevalap, H. Shim, J. P. Katz, V. W. Yang, and C. C. Yun
Lysophosphatidic Acid Facilitates Proliferation of Colon Cancer Cells via Induction of Kruppel-like Factor 5
J. Biol. Chem., May 25, 2007; 282(21): 15541 - 15549.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. J. DiNubile
Plasma gelsolin: in search of its raison d'etre. Focus on "Modifications of cellular responses to lysophosphatidic acid and platelet-activating factor by plasma gelsolin"
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1240 - C1242.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. M. Osborn, C. Dahlgren, J. H. Hartwig, and T. P. Stossel
Modifications of cellular responses to lysophosphatidic acid and platelet-activating factor by plasma gelsolin
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1323 - C1330.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
D. Hollenback, L. Bonham, L. Law, E. Rossnagle, L. Romero, H. Carew, C. K. Tompkins, D. W. Leung, J. W. Singer, and T. White
Substrate specificity of lysophosphatidic acid acyltransferase {beta}--evidence from membrane and whole cell assays
J. Lipid Res., March 1, 2006; 47(3): 593 - 604.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
H. Takahashi, Y. Takeishi, T. Seidler, T. Arimoto, H. Akiyama, Y. Hozumi, Y. Koyama, T. Shishido, Y. Tsunoda, T. Niizeki, et al.
Adenovirus-Mediated Overexpression of Diacylglycerol Kinase-{zeta} Inhibits Endothelin-1-Induced Cardiomyocyte Hypertrophy
Circulation, March 29, 2005; 111(12): 1510 - 1516.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Bagga, K. S. Price, D. A. Lin, D. S. Friend, K. F. Austen, and J. A. Boyce
Lysophosphatidic acid accelerates the development of human mast cells
Blood, December 15, 2004; 104(13): 4080 - 4087.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Cummings, Y. Zhao, D. Jacoby, E. W. Spannhake, M. Ohba, J. G. N. Garcia, T. Watkins, D. He, B. Saatian, and V. Natarajan
Protein Kinase C{delta} Mediates Lysophosphatidic Acid-induced NF-{kappa}B Activation and Interleukin-8 Secretion in Human Bronchial Epithelial Cells
J. Biol. Chem., September 24, 2004; 279(39): 41085 - 41094.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. A. Rothenbach, B. Dahl, J. J. Schwartz, G. E. O'Keefe, M. Yamamoto, W. M. Lee, J. W. Horton, H. L. Yin, and R. H. Turnage
Recombinant plasma gelsolin infusion attenuates burn-induced pulmonary microvascular dysfunction
J Appl Physiol, January 1, 2004; 96(1): 25 - 31.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. J. DiNubile, T. P. Stossel, O. C. Ljunghusen, J. L. M. Ferrara, and J. H. Antin
Prognostic implications of declining plasma gelsolin levels after allogeneic stem cell transplantation
Blood, December 15, 2002; 100(13): 4367 - 4371.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Sano, D. Baker, T. Virag, A. Wada, Y. Yatomi, T. Kobayashi, Y. Igarashi, and G. Tigyi
Multiple Mechanisms Linked to Platelet Activation Result in Lysophosphatidic Acid and Sphingosine 1-Phosphate Generation in Blood
J. Biol. Chem., June 7, 2002; 277(24): 21197 - 21206.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Z.-Q. Jin, H.-Z. Zhou, P. Zhu, N. Honbo, D. Mochly-Rosen, R. O. Messing, E. J. Goetzl, J. S. Karliner, and M. O. Gray
Cardioprotection mediated by sphingosine-1-phosphate and ganglioside GM-1 in wild-type and PKCepsilon knockout mouse hearts
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H1970 - H1977.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Z.-Q. Jin, H.-Z. Zhou, P. Zhu, N. Honbo, D. Mochly-Rosen, R. O. Messing, E. J. Goetzl, J. S. Karliner, and M. O. Gray
Cardioprotection mediated by sphingosine-1-phosphate and ganglioside GM-1 in wild-type and PKCepsilon knockout mouse hearts
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H1970 - H1977.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.