| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 19, 14700-14707, May 12, 2000
From the Departments of To identify genes that are transcriptionally
activated when human macrophages accumulate excess lipids, we employed
the mRNA differential display technique using RNA isolated from
human monocyte-macrophages incubated in the absence or presence of
acetylated low density lipoprotein and sterols (cholesterol and
25-hydroxycholesterol). These studies identified a mRNA whose
levels were highly induced in lipid-loaded macrophages. The mRNA
encoded the human White protein, a member of the ATP-binding cassette
(ABC) transporter superfamily of proteins. The mRNA levels of ABC8,
the murine homolog of the human white gene, were also
induced when a murine macrophage cell line, RAW264.7, was incubated
with acetylated low density lipoprotein and sterols. Additional studies
demonstrated that white/ABC8 mRNA levels were induced by specific
oxysterols that included 25-, 20(S)-, and
22(R)-hydroxycholesterol, and by a retinoid X
receptor-specific ligand. Furthermore, the oxysterol-mediated induction
of ABC8 expression in mouse peritoneal macrophages was dependent on the
presence of the nuclear oxysterol receptors, liver X receptors (LXRs).
Macrophages derived from mice lacking both LXR
Human White/Murine ABC8 mRNA Levels Are Highly Induced in
Lipid-loaded Macrophages
A TRANSCRIPTIONAL ROLE FOR SPECIFIC OXYSTEROLS*
,
, and**§§
Biological Chemistry and
Medicine and the ** Molecular Biology Institute, University of
California, Los Angeles, Los Angeles, California 90095 and the
§ Howard Hughes Medical Institute and Department of
Pharmacology, University of Texas Southwestern Medical Center,
Dallas, Texas 75390-9050
and LXR
failed to
up-regulate the expression of ABC8 following incubation with
22(R)-hydroxycholesterol. Oxysterol-dependent induction of white/ABC8 mRNA was blocked by actinomycin D but not
by cycloheximide treatment of cells. We conclude that the white and ABC8 genes are primary response genes
that are transcriptionally activated by specific oxysterols and that
this induction is mediated by the LXR subfamily of nuclear hormone
receptors. These data strongly support the hypothesis that
white/ABC8 has a role in cellular sterol homeostasis.
*
This work was supported by Grant HL 30568 (to P. A. E.) from the National Institutes of Health, grants from the
Laubisch Fund (to P. A. E.), and the Howard Hughes Medical
Institute (to D. J. M.). J.-M. A. L. received an installation
grant from the Philippe Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Investigator for the Howard Hughes Medical Institute.
§§
To whom correspondence should be addressed: Dept. of Biological
Chemistry, CHS 33-257, UCLA, Los Angeles, CA 90095-1769. Tel.: 310-206-3717; Fax: 310-794-7345; E-mail:
pedwards@mednet.UCLA.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. J. Field, K. Watt, and S. N. Mathur Origins of intestinal ABCA1-mediated HDL-cholesterol J. Lipid Res., December 1, 2008; 49(12): 2605 - 2619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Lindegaard, C. A. Wassif, B. Vaisman, M. Amar, E. V. Wasmuth, R. Shamburek, L. B. Nielsen, A. T. Remaley, and F. D. Porter Characterization of placental cholesterol transport: ABCA1 is a potential target for in utero therapy of Smith-Lemli-Opitz syndrome Hum. Mol. Genet., December 1, 2008; 17(23): 3806 - 3813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Colin, E. Bourguignon, A.-B. Boullay, J.-J. Tousaint, S. Huet, F. Caira, B. Staels, S. Lestavel, J.-M. A. Lobaccaro, and P. Delerive Intestine-Specific Regulation of PPAR{alpha} Gene Transcription by Liver X Receptors Endocrinology, October 1, 2008; 149(10): 5128 - 5135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Burgess, K. Naus, J. Chan, V. Hirsch-Reinshagen, G. Tansley, L. Matzke, B. Chan, A. Wilkinson, J. Fan, J. Donkin, et al. Overexpression of Human ABCG1 Does Not Affect Atherosclerosis in Fat-Fed ApoE-Deficient Mice Arterioscler. Thromb. Vasc. Biol., October 1, 2008; 28(10): 1731 - 1737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Bowden and N. D. Ridgway OSBP Negatively Regulates ABCA1 Protein Stability J. Biol. Chem., June 27, 2008; 283(26): 18210 - 18217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ouimet, M.-D. Wang, N. Cadotte, K. Ho, and Y. L. Marcel Epoxycholesterol Impairs Cholesteryl Ester Hydrolysis in Macrophage Foam Cells, Resulting in Decreased Cholesterol Efflux Arterioscler. Thromb. Vasc. Biol., June 1, 2008; 28(6): 1144 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Baldan, A. V. Gomes, P. Ping, and P. A. Edwards Loss of ABCG1 Results in Chronic Pulmonary Inflammation J. Immunol., March 1, 2008; 180(5): 3560 - 3568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. T. Tarr and P. A. Edwards ABCG1 and ABCG4 are coexpressed in neurons and astrocytes of the CNS and regulate cholesterol homeostasis through SREBP-2 J. Lipid Res., January 1, 2008; 49(1): 169 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yvan-Charvet, T. A. Pagler, N. Wang, T. Senokuchi, M. Brundert, H. Li, F. Rinninger, and A. R. Tall SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL J. Lipid Res., January 1, 2008; 49(1): 107 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K.-H. Chow, B. Razani, and G. Cheng Innate immune system regulation of nuclear hormone receptors in metabolic diseases J. Leukoc. Biol., August 1, 2007; 82(2): 187 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mouzat, M. Prod'Homme, D. H. Volle, B. Sion, P. Dechelotte, K. Gauthier, J.-M. Vanacker, and J.-M. A. Lobaccaro Oxysterol Nuclear Receptor LXRbeta Regulates Cholesterol Homeostasis and Contractile Function in Mouse Uterus J. Biol. Chem., February 16, 2007; 282(7): 4693 - 4701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Moore, S. Kato, W. Xie, D. J. Mangelsdorf, D. R. Schmidt, R. Xiao, and S. A. Kliewer International Union of Pharmacology. LXII. The NR1H and NR1I Receptors: Constitutive Androstane Receptor, Pregnene X Receptor, Farnesoid X Receptor {alpha}, Farnesoid X Receptor beta, Liver X Receptor {alpha}, Liver X Receptor beta, and Vitamin D Receptor Pharmacol. Rev., December 1, 2006; 58(4): 742 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Oram and A. M. Vaughan ATP-Binding Cassette Cholesterol Transporters and Cardiovascular Disease Circ. Res., November 10, 2006; 99(10): 1031 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Vaughan and J. F. Oram ABCA1 and ABCG1 or ABCG4 act sequentially to remove cellular cholesterol and generate cholesterol-rich HDL J. Lipid Res., November 1, 2006; 47(11): 2433 - 2443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Out, M. Hoekstra, R. B. Hildebrand, J. K. Kruit, I. Meurs, Z. Li, F. Kuipers, T. J.C. Van Berkel, and M. Van Eck Macrophage ABCG1 Deletion Disrupts Lipid Homeostasis in Alveolar Macrophages and Moderately Influences Atherosclerotic Lesion Development in LDL Receptor-Deficient Mice Arterioscler. Thromb. Vasc. Biol., October 1, 2006; 26(10): 2295 - 2300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baldan, P. Tarr, C. S. Vales, J. Frank, T. K. Shimotake, S. Hawgood, and P. A. Edwards Deletion of the Transmembrane Transporter ABCG1 Results in Progressive Pulmonary Lipidosis J. Biol. Chem., September 29, 2006; 281(39): 29401 - 29410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kobayashi, Y. Takanezawa, T. Hirata, Y. Shimizu, K. Misasa, N. Kioka, H. Arai, K. Ueda, and M. Matsuo Efflux of sphingomyelin, cholesterol, and phosphatidylcholine by ABCG1 J. Lipid Res., August 1, 2006; 47(8): 1791 - 1802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Duffy and D. J. Rader Emerging Therapies Targeting High-Density Lipoprotein Metabolism and Reverse Cholesterol Transport Circulation, February 28, 2006; 113(8): 1140 - 1150. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Majdalawieh, L. Zhang, I. V. Fuki, D. J. Rader, and H.-S. Ro Adipocyte enhancer-binding protein 1 is a potential novel atherogenic factor involved in macrophage cholesterol homeostasis and inflammation PNAS, February 14, 2006; 103(7): 2346 - 2351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ayaori, S. Sawada, A. Yonemura, N. Iwamoto, M. Ogura, N. Tanaka, K. Nakaya, M. Kusuhara, H. Nakamura, and F. Ohsuzu Glucocorticoid Receptor Regulates ATP-Binding Cassette Transporter-A1 Expression and Apolipoprotein-Mediated Cholesterol Efflux From Macrophages Arterioscler. Thromb. Vasc. Biol., January 1, 2006; 26(1): 163 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chen, S. Beaven, and P. Tontonoz Identification and characterization of two alternatively spliced transcript variants of human liver X receptor alpha J. Lipid Res., December 1, 2005; 46(12): 2570 - 2579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Grefhorst, T. H. van Dijk, A. Hammer, F. H. van der Sluijs, R. Havinga, L. M. Havekes, J. A. Romijn, P. H. Groot, D.-J. Reijngoud, and F. Kuipers Differential effects of pharmacological liver X receptor activation on hepatic and peripheral insulin sensitivity in lean and ob/ob mice Am J Physiol Endocrinol Metab, November 1, 2005; 289(5): E829 - E838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Sabol, H. B. Brewer Jr., and S. Santamarina-Fojo The human ABCG1 gene: identification of LXR response elements that modulate expression in macrophages and liver J. Lipid Res., October 1, 2005; 46(10): 2151 - 2167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Vaughan and J. F. Oram ABCG1 Redistributes Cell Cholesterol to Domains Removable by High Density Lipoprotein but Not by Lipid-depleted Apolipoproteins J. Biol. Chem., August 26, 2005; 280(34): 30150 - 30157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. I. Shulman and D. J. Mangelsdorf Retinoid X Receptor Heterodimers in the Metabolic Syndrome N. Engl. J. Med., August 11, 2005; 353(6): 604 - 615. [Full Text] [PDF]
|
|
|
|
|
|
A. C. Li and C. K. Glass PPAR- and LXR-dependent pathways controlling lipid metabolism and the development of atherosclerosis J. Lipid Res., December 1, 2004; 45(12): 2161 - 2173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. O'Connell, M. Denis, and J. Genest Cellular Physiology of Cholesterol Efflux in Vascular Endothelial Cells Circulation, November 2, 2004; 110(18): 2881 - 2888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Ulven, K. T. Dalen, J.-A. Gustafsson, and H. I. Nebb Tissue-specific autoregulation of the LXR{alpha} gene facilitates induction of apoE in mouse adipose tissue J. Lipid Res., November 1, 2004; 45(11): 2052 - 2062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakamura, M. A. Kennedy, A. Baldan, D. D. Bojanic, K. Lyons, and P. A. Edwards Expression and Regulation of Multiple Murine ATP-binding Cassette Transporter G1 mRNAs/Isoforms That Stimulate Cellular Cholesterol Efflux to High Density Lipoprotein J. Biol. Chem., October 29, 2004; 279(44): 45980 - 45989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J-M Frenoux, P Vernet, D H Volle, A Britan, F Saez, A Kocer, J Henry-Berger, D J Mangelsdorf, J-M A Lobaccaro, and J R Drevet Nuclear oxysterol receptors, LXRs, are involved in the maintenance of mouse caput epididymidis structure and functions J. Mol. Endocrinol., October 1, 2004; 33(2): 361 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Quinet, D. A. Savio, A. R. Halpern, L. Chen, C. P. Miller, and P. Nambi Gene-selective modulation by a synthetic oxysterol ligand of the liver X receptor J. Lipid Res., October 1, 2004; 45(10): 1929 - 1942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ando, S. Tsuruoka, H. Yamamoto, T. Takamura, S. Kaneko, and A. Fujimura Effects of Pravastatin on the Expression of ATP-Binding Cassette Transporter A1 J. Pharmacol. Exp. Ther., October 1, 2004; 311(1): 420 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-P. Duan, H. H. Wang, and D. Q-H. Wang Cholesterol absorption is mainly regulated by the jejunal and ileal ATP-binding cassette sterol efflux transporters Abcg5 and Abcg8 in mice J. Lipid Res., July 1, 2004; 45(7): 1312 - 1323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yu, G. Cao, J. Repa, and H. Stangl Sterol regulation of scavenger receptor class B type I in macrophages J. Lipid Res., May 1, 2004; 45(5): 889 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Seo, H. M. Moon, W. S. Kim, Y. S. Lee, H. W. Jeong, E. J. Yoo, J. Ham, H. Kang, M.-G. Park, K. R. Steffensen, et al. Activated Liver X Receptors Stimulate Adipocyte Differentiation through Induction of Peroxisome Proliferator-Activated Receptor {gamma} Expression Mol. Cell. Biol., April 15, 2004; 24(8): 3430 - 3444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang, J. J. Repa, Y. Inoue, G. P. Hayhurst, F. J. Gonzalez, and D. J. Mangelsdorf Identification of a Liver-Specific Uridine Phosphorylase that Is Regulated by Multiple Lipid-Sensing Nuclear Receptors Mol. Endocrinol., April 1, 2004; 18(4): 851 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Walczak, S. B. Joseph, B. A. Laffitte, A. Castrillo, L. Pei, and P. Tontonoz Transcription of the Vascular Endothelial Growth Factor Gene in Macrophages Is Regulated by Liver X Receptors J. Biol. Chem., March 12, 2004; 279(11): 9905 - 9911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Suzuki, T. Nishimaki-Mogami, N. Tamehiro, K. Inoue, R. Arakawa, S. Abe-Dohmae, A. R. Tanaka, K. Ueda, and S. Yokoyama Verapamil Increases the Apolipoprotein-Mediated Release of Cellular Cholesterol by Induction of ABCA1 Expression Via Liver X Receptor-Independent Mechanism Arterioscler. Thromb. Vasc. Biol., March 1, 2004; 24(3): 519 - 525. [Abstract] [Full Text]
|
|
|
|
|
|
M. Ricote, A. F. Valledor, and C. K. Glass Decoding Transcriptional Programs Regulated by PPARs and LXRs in the Macrophage: Effects on Lipid Homeostasis, Inflammation, and Atherosclerosis Arterioscler. Thromb. Vasc. Biol., February 1, 2004; 24(2): 230 - 239. [Abstract] [Full Text]
|
|
|
|
|
|
K. T. Dalen, S. M. Ulven, K. Bamberg, J.-A. Gustafsson, and H. I. Nebb Expression of the Insulin-responsive Glucose Transporter GLUT4 in Adipocytes Is Dependent on Liver X Receptor {alpha} J. Biol. Chem., November 28, 2003; 278(48): 48283 - 48291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Costet, F. Lalanne, M. C. Gerbod-Giannone, J. R. Molina, X. Fu, E. G. Lund, L. J. Gudas, and A. R. Tall Retinoic Acid Receptor-Mediated Induction of ABCA1 in Macrophages Mol. Cell. Biol., November 1, 2003; 23(21): 7756 - 7766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Farnegardh, T. Bonn, S. Sun, J. Ljunggren, H. Ahola, A. Wilhelmsson, J.-A. Gustafsson, and M. Carlquist The Three-dimensional Structure of the Liver X Receptor {beta} Reveals a Flexible Ligand-binding Pocket That Can Accommodate Fundamentally Different Ligands J. Biol. Chem., October 3, 2003; 278(40): 38821 - 38828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Bramlett, K. A. Houck, K. M. Borchert, M. S. Dowless, P. Kulanthaivel, Y. Zhang, T. P. Beyer, R. Schmidt, J. S. Thomas, L. F. Michael, et al. A Natural Product Ligand of the Oxysterol Receptor, Liver X Receptor J. Pharmacol. Exp. Ther., October 1, 2003; 307(1): 291 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Wagner, A. F. Valledor, G. Shao, C. L. Daige, E. D. Bischoff, M. Petrowski, K. Jepsen, S. H. Baek, R. A. Heyman, M. G. Rosenfeld, et al. Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 and SREBP1 Gene Expression Mol. Cell. Biol., August 15, 2003; 23(16): 5780 - 5789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. G. Lund, J. G. Menke, and C. P. Sparrow Liver X Receptor Agonists as Potential Therapeutic Agents for Dyslipidemia and Atherosclerosis Arterioscler. Thromb. Vasc. Biol., July 1, 2003; 23(7): 1169 - 1177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Tontonoz and D. J. Mangelsdorf Liver X Receptor Signaling Pathways in Cardiovascular Disease Mol. Endocrinol., June 1, 2003; 17(6): 985 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. Kim, K. Park, E. Kwak, E. Choi, S. Lee, J. Ham, H. Kang, J. M. Kim, S. Y. Hwang, Y.-Y. Kong, et al. Activating Signal Cointegrator 2 Required for Liver Lipid Metabolism Mediated by Liver X Receptors in Mice Mol. Cell. Biol., May 15, 2003; 23(10): 3583 - 3592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Laffitte, L. C. Chao, J. Li, R. Walczak, S. Hummasti, S. B. Joseph, A. Castrillo, D. C. Wilpitz, D. J. Mangelsdorf, J. L. Collins, et al. Activation of liver X receptor improves glucose tolerance through coordinate regulation of glucose metabolism in liver and adipose tissue PNAS, April 29, 2003; 100(9): 5419 - 5424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lee, J. Metso, M. Jauhiainen, and P. T. Kovanen Degradation of Phospholipid Transfer Protein (PLTP) and PLTP-generated Pre-beta -high Density Lipoprotein by Mast Cell Chymase Impairs High Affinity Efflux of Cholesterol from Macrophage Foam Cells J. Biol. Chem., April 4, 2003; 278(15): 13539 - 13545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Laffitte, S. B. Joseph, M. Chen, A. Castrillo, J. Repa, D. Wilpitz, D. Mangelsdorf, and P. Tontonoz The Phospholipid Transfer Protein Gene Is a Liver X Receptor Target Expressed by Macrophages in Atherosclerotic Lesions Mol. Cell. Biol., March 15, 2003; 23(6): 2182 - 2191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Juvet, S. M. Andresen, G. U. Schuster, K. T. Dalen, K. A. R. Tobin, K. Hollung, F. Haugen, S. Jacinto, S. M. Ulven, K. Bamberg, et al. On the Role of Liver X Receptors in Lipid Accumulation in Adipocytes Mol. Endocrinol., February 1, 2003; 17(2): 172 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kaplan, T. Zhang, M. Hernandez, F. X. Gan, S. D. Wright, M. G. Waters, and T.-Q. Cai Regulation of the angiopoietin-like protein 3 gene by LXR J. Lipid Res., January 1, 2003; 44(1): 136 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. R. Su, M. F. Linton, and S. Fazio Rapid quantification of murine ABC mRNAs by real time reverse transcriptase-polymerase chain reaction J. Lipid Res., December 1, 2002; 43(12): 2180 - 2187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Stulnig, K. R. Steffensen, H. Gao, M. Reimers, K. Dahlman-Wright, G. U. Schuster, and J.-A. Gustafsson Novel Roles of Liver X Receptors Exposed by Gene Expression Profiling in Liver and Adipose Tissue Mol. Pharmacol., December 1, 2002; 62(6): 1299 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
< |
