![]()
|
|
||||||||
J Biol Chem, Vol. 275, Issue 2, 1023-1029, January 14, 2000
From the The absorption of dietary non-heme iron by
intestinal enterocytes is crucial to the maintenance of body iron
homeostasis. This process must be tightly regulated since there are no
distinct mechanisms for the excretion of excess iron from the body. An insight into the cellular mechanisms has recently been provided by
expression cloning of a divalent cation transporter (DCT1) from rat
duodenum and positional cloning of its human homologue, Nramp2. Here we
demonstrate that Nramp2 is expressed in the apical membrane of the
human intestinal epithelial cell line, Caco 2 TC7, and is associated
with functional iron transport in these cells with a substrate
preference for iron over other divalent cations. Iron transport
occurs by a proton-dependent mechanism, exhibiting a
concurrent intracellular acidification. Taken together, these data
suggest that the expression of the Nramp2 transporter in human
enterocytes may play an important role in intestinal iron absorption.
Nramp2 Expression Is Associated with pH-dependent
Iron Uptake across the Apical Membrane of Human Intestinal Caco-2
Cells*
§,
¶,
,
,
,
,
, and
¶**
School of Biological Sciences, University of
East Anglia, Norwich, NR4 7TJ, ¶ Institute of Food Research,
Norwich, NR4 7UA, and
Department of Biochemistry and Molecular
Biology, Royal Free Hospital and University College Medical School,
London NW3 2PF, United Kingdom
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
This article has been cited by other articles:
![]() |
G. O. Latunde-Dada, R. J. Simpson, and A. T. McKie Duodenal Cytochrome B Expression Stimulates Iron Uptake by Human Intestinal Epithelial Cells J. Nutr., June 1, 2008; 138(6): 991 - 995. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. M. Zimnicka, E. B. Maryon, and J. H. Kaplan Human Copper Transporter hCTR1 Mediates Basolateral Uptake of Copper into Enterocytes: IMPLICATIONS FOR COPPER HOMEOSTASIS J. Biol. Chem., September 7, 2007; 282(36): 26471 - 26480. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. T. Thwaites and C. M. H. Anderson H+-coupled nutrient, micronutrient and drug transporters in the mammalian small intestine Exp Physiol, July 1, 2007; 92(4): 603 - 619. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y.-Z. Chang, Y. Ke, J.-R. Du, G. M. Halpern, K.-P. Ho, L. Zhu, X.-S. Gu, Y.-J. Xu, Q. Wang, L.-Z. Li, et al. Increased Divalent Metal Transporter 1 Expression Might Be Associated with the Neurotoxicity of L-DOPA Mol. Pharmacol., March 1, 2006; 69(3): 968 - 974. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. F. Eisses and J. H. Kaplan The Mechanism of Copper Uptake Mediated by Human CTR1: A MUTATIONAL ANALYSIS J. Biol. Chem., November 4, 2005; 280(44): 37159 - 37168. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. P. Mims, Y. Guan, D. Pospisilova, M. Priwitzerova, K. Indrak, P. Ponka, V. Divoky, and J. T. Prchal Identification of a human mutation of DMT1 in a patient with microcytic anemia and iron overload Blood, February 1, 2005; 105(3): 1337 - 1342. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Wagner, J. Maser, I. Moric, N. Boechat, S. Vogt, B. Gicquel, B. Lai, J.-M. Reyrat, and L. Bermudez Changes of the phagosomal elemental concentrations by Mycobacterium tuberculosis Mramp Microbiology, January 1, 2005; 151(1): 323 - 332. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Nevo and N. Nelson The Mutation F227I Increases the Coupling of Metal Ion Transport in DCT1 J. Biol. Chem., December 17, 2004; 279(51): 53056 - 53061. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Yamaji, P. Sharp, B. Ramesh, and S. K. Srai Inhibition of iron transport across human intestinal epithelial cells by hepcidin Blood, October 1, 2004; 104(7): 2178 - 2180. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Arredondo, V. Cambiazo, L. Tapia, M. Gonzalez-Aguero, M. T. Nunez, R. Uauy, and M. Gonzalez Copper overload affects copper and iron metabolism in Hep-G2 cells Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G27 - G32. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Kordas and R. J. Stoltzfus New Evidence of Iron and Zinc Interplay at the Enterocyte and Neural Tissues J. Nutr., June 1, 2004; 134(6): 1295 - 1298. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Barisani, A. Parafioriti, M. T. Bardella, H. Zoller, D. Conte, E. Armiraglio, C. Trovato, R. O. Koch, and G. Weiss Adaptive changes of duodenal iron transport proteins in celiac disease Physiol Genomics, May 19, 2004; 17(3): 316 - 325. [Abstract] [Full Text] [PDF] |
||||
![]() |
C Thomas and P S Oates Ferroportin/IREG-1/MTP-1/SLC40A1 modulates the uptake of iron at the apical membrane of enterocytes Gut, January 1, 2004; 53(1): 44 - 49. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Wareing, C. J. Ferguson, M. Delannoy, A. G. Cox, R. F. T. McMahon, R. Green, D. Riccardi, and C. P. Smith Altered dietary iron intake is a strong modulator of renal DMT1 expression Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1050 - F1059. [Abstract] [Full Text] |
||||
![]() |
B. D. Schneider and E. A. Leibold Effects of iron regulatory protein regulation on iron homeostasis during hypoxia Blood, November 1, 2003; 102(9): 3404 - 3411. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. R. Forbes and P. Gros Iron, manganese, and cobalt transport by Nramp1 (Slc11a1) and Nramp2 (Slc11a2) expressed at the plasma membrane Blood, September 1, 2003; 102(5): 1884 - 1892. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Touret, W. Furuya, J. Forbes, P. Gros, and S. Grinstein Dynamic Traffic through the Recycling Compartment Couples the Metal Transporter Nramp2 (DMT1) with the Transferrin Receptor J. Biol. Chem., July 3, 2003; 278(28): 25548 - 25557. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Arredondo, P. Munoz, C. V. Mura, and M. T. Nunez DMT1, a physiologically relevant apical Cu1+ transporter of intestinal cells Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1525 - C1530. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Jabado, P. Cuellar-Mata, S. Grinstein, and P. Gros Iron chelators modulate the fusogenic properties of Salmonella-containing phagosomes PNAS, May 13, 2003; 100(10): 6127 - 6132. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Lam-Yuk-Tseung, G. Govoni, J. Forbes, and P. Gros Iron transport by Nramp2/DMT1: pH regulation of transport by 2 histidines in transmembrane domain 6 Blood, May 1, 2003; 101(9): 3699 - 3707. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. G. Gehrke, H.-D. Riedel, T. Herrmann, B. Hadaschik, K. Bents, C. Veltkamp, and W. Stremmel UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis Blood, April 15, 2003; 101(8): 3288 - 3293. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Chahdi, W. S. Choi, Y. M. Kim, and M. A. Beaven Mastoparan Selectively Activates Phospholipase D2 in Cell Membranes J. Biol. Chem., March 28, 2003; 278(14): 12039 - 12045. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. I. Bannon, R. Abounader, P. S. J. Lees, and J. P. Bressler Effect of DMT1 knockdown on iron, cadmium, and lead uptake in Caco-2 cells Am J Physiol Cell Physiol, January 1, 2003; 284(1): C44 - C50. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Tabuchi, N. Tanaka, J. Nishida-Kitayama, H. Ohno, and F. Kishi Alternative Splicing Regulates the Subcellular Localization of Divalent Metal Transporter 1 Isoforms Mol. Biol. Cell, December 1, 2002; 13(12): 4371 - 4387. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. La Fontaine, J. M. Quinn, S. S. Nakamoto, M. D. Page, V. Gohre, J. L. Moseley, J. Kropat, and S. Merchant Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii Eukaryot. Cell, October 1, 2002; 1(5): 736 - 757. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Ma, R. D. Specian, K.-Y. Yeh, M. Yeh, J. Rodriguez-Paris, and J. Glass The transcytosis of divalent metal transporter 1 and apo-transferrin during iron uptake in intestinal epithelium Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G965 - G974. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Jabado, F. Canonne-Hergaux, S. Gruenheid, V. Picard, and P. Gros Iron transporter Nramp2/DMT-1 is associated with the membrane of phagosomes in macrophages and Sertoli cells Blood, September 18, 2002; 100(7): 2617 - 2622. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. D. Park, N. J. Cherrington, and C. D. Klaassen Intestinal Absorption of Cadmium Is Associated with Divalent Metal Transporter 1 in Rats Toxicol. Sci., August 1, 2002; 68(2): 288 - 294. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. A. Cragg, G. R. Christie, S. R. Phillips, R. M. Russi, S. Kury, J. C. Mathers, P. M. Taylor, and D. Ford A Novel Zinc-regulated Human Zinc Transporter, hZTL1, Is Localized to the Enterocyte Apical Membrane J. Biol. Chem., June 14, 2002; 277(25): 22789 - 22797. [Abstract] [Full Text] [PDF] |
||||
![]() |
W. S. Choi, Y. M. Kim, C. Combs, M. A. Frohman, and M. A. Beaven Phospholipases D1 and D2 Regulate Different Phases of Exocytosis in Mast Cells J. Immunol., June 1, 2002; 168(11): 5682 - 5689. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Thomas and P. S. Oates IEC-6 Cells Are an Appropriate Model of Intestinal Iron Absorption in Rats J. Nutr., April 1, 2002; 132(4): 680 - 687. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. Lee, M. M. O. Pena, Y. Nose, and D. J. Thiele Biochemical Characterization of the Human Copper Transporter Ctr1 J. Biol. Chem., February 1, 2002; 277(6): 4380 - 4387. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. N. Glover and C. Hogstrand In vivo characterisation of intestinal zinc uptake in freshwater rainbow trout J. Exp. Biol., January 1, 2002; 205(1): 141 - 150. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. Canonne-Hergaux, A.-S. Zhang, P. Ponka, and P. Gros Characterization of the iron transporter DMT1 (NRAMP2/DCT1) in red blood cells of normal and anemic mk/mk mice Blood, December 15, 2001; 98(13): 3823 - 3830. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. K. Lund, S. J. Fairweather-Tait, S. G. Wharf, and I. T. Johnson Chronic Exposure to High Levels of Dietary Iron Fortification Increases Lipid Peroxidation in the Mucosa of the Rat Large Intestine J. Nutr., November 1, 2001; 131(11): 2928 - 2931. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. Olivi, J. Sisk, and J. Bressler Involvement of DMT1 in uptake of Cd in MDCK cells: role of protein kinase C Am J Physiol Cell Physiol, September 1, 2001; 281(3): C793 - C800. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. J. Fairweather-Tait Iron J. Nutr., April 1, 2001; 131(4): 1383S - 1386. [Abstract] [Full Text] |
||||
![]() |
A Sacher, A Cohen, and N Nelson Properties of the mammalian and yeast metal-ion transporters DCT1 and Smf1p expressed in Xenopus laevis oocytes J. Exp. Biol., January 3, 2001; 204(6): 1053 - 1061. [Abstract] [PDF] |
||||
![]() |
C. N. Roy and C. A. Enns Iron homeostasis: new tales from the crypt Blood, December 15, 2000; 96(13): 4020 - 4027. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. T. Worthington, L. Browne, E. H. Battle, and R. Q. Luo Functional properties of transfected human DMT1 iron transporter Am J Physiol Gastrointest Liver Physiol, December 1, 2000; 279(6): G1265 - G1273. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Jabado, A. Jankowski, S. Dougaparsad, V. Picard, S. Grinstein, and P. Gros Natural resistance to intracellular infections: natural resistance-associated macrophage protein 1 (NRAMP1) functions as a pH-dependent manganese transporter at the phagosomal membrane J. Exp. Med., October 30, 2000; 192(9): 1237 - 1248. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. E. Conrad, J. N. Umbreit, E. G. Moore, L. N. Hainsworth, M. Porubcin, M. J. Simovich, M. T. Nakada, K. Dolan, and M. D. Garrick Separate pathways for cellular uptake of ferric and ferrous iron Am J Physiol Gastrointest Liver Physiol, October 1, 2000; 279(4): G767 - G774. [Abstract] [Full Text] [PDF] |
||||
![]() |
V. Picard, G. Govoni, N. Jabado, and P. Gros Nramp 2 (DCT1/DMT1) Expressed at the Plasma Membrane Transports Iron and Other Divalent Cations into a Calcein-accessible Cytoplasmic Pool J. Biol. Chem., November 10, 2000; 275(46): 35738 - 35745. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |