| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 2, 1065-1072, January 14, 2000
From the Department of Pathology, University of Alabama at
Birmingham, Birmingham, Alabama 35294 and Bone morphogenetic proteins are potent
osteotropic agents that induce osteoblast differentiation and bone
formation. The signal transduction of bone morphogenetic proteins has
recently been discovered to involve Smad proteins. Smad1 is an
essential intracellular component that is specifically phosphorylated
by bone morphogenetic protein receptors and translocated into the
nucleus upon ligand stimulation. Previously, we have reported that
Smad1 activates osteopontin gene expression in response to bone
morphogenetic protein simulation through an interaction with a
homeodomain transcription factor, Hoxc-8. In the present study, the
interaction domains between the two proteins were characterized
by deletional analysis in both yeast two-hybrid and gel shift assays.
Two regions within the amino-terminal 87 amino acid residues of Smad1
were mapped to interact with Hoxc-8, one of which binds to the
homeodomain. Overexpression of recombinant cDNAs encoding the
Hoxc-8 interaction domains of Smad1 effectively activated osteopontin
gene transcription in transient transfection assays. Furthermore,
stable expression of these Smad1 fragments in 2T3 osteoblast precursor
cells stimulated osteoblast differentiation-related gene expression and
led to mineralized bone matrix formation. Our data suggest that the
interaction of amino-terminal Smad1 with Hoxc-8 mimics bone
morphogenetic protein signaling and is sufficient to induce osteoblast
differentiation and bone cell formation.
Smad1 Domains Interacting with Hoxc-8 Induce Osteoblast
Differentiation*
, Procter & Gamble Pharmaceuticals, Mason, Ohio 45040
*
This work was supported in part by National Institutes of
Health Grant DK53757 (to X. C).The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Hayashi, K. Nimura, K. Kashiwagi, T. Harada, K. Takaoka, H. Kato, K. Tamai, and Y. Kaneda Comparative roles of Twist-1 and Id1 in transcriptional regulation by BMP signaling J. Cell Sci., April 15, 2007; 120(8): 1350 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Grocott, V. Frost, M. Maillard, T. Johansen, G. N. Wheeler, L. J. Dawes, I. M. Wormstone, and A. Chantry The MH1 domain of Smad3 interacts with Pax6 and represses autoregulation of the Pax6 P1 promoter Nucleic Acids Res., February 16, 2007; 35(3): 890 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Liu, Y. Tang, T. Qiu, X. Cao, and T. L. Clemens A Dishevelled-1/Smad1 Interaction Couples WNT and Bone Morphogenetic Protein Signaling Pathways in Uncommitted Bone Marrow Stromal Cells J. Biol. Chem., June 23, 2006; 281(25): 17156 - 17163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Bondos, X.-X. Tan, and K. S. Matthews Physical and Genetic Interactions Link Hox Function with Diverse Transcription Factors and Cell Signaling Proteins Mol. Cell. Proteomics, May 1, 2006; 5(5): 824 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Helvering, R. Liu, N. H. Kulkarni, T. Wei, P. Chen, S. Huang, F. Lawrence, D. L. Halladay, R. R. Miles, E. M. Ambrose, et al. Expression Profiling of Rat Femur Revealed Suppression of Bone Formation Genes by Treatment with Alendronate and Estrogen but Not Raloxifene Mol. Pharmacol., November 1, 2005; 68(5): 1225 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Williams, M. E. Williams, J. H. Heaton, T. D. Gelehrter, and J. W. Innis Group 13 HOX proteins interact with the MH2 domain of R-Smads and modulate Smad transcriptional activation functions independent of HOX DNA-binding capability Nucleic Acids Res., August 8, 2005; 33(14): 4475 - 4484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Pardo, M. J. Patel, M. C. Sykes, M. O. Platt, N. L. Boyd, G. P. Sorescu, M. Xu, J. J. W. A. van Loon, M. D. Wang, and H. Jo Simulated microgravity using the Random Positioning Machine inhibits differentiation and alters gene expression profiles of 2T3 preosteoblasts Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1211 - C1221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takahashi, H. Abe, H. Arai, T. Matsubara, K. Nagai, M. Matsuura, N. Iehara, M. Yokode, S. Nishikawa, T. Kita, et al. Activation of STAT3/Smad1 Is a Key Signaling Pathway for Progression to Glomerulosclerosis in Experimental Glomerulonephritis J. Biol. Chem., February 25, 2005; 280(8): 7100 - 7106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. El-Tanani, A. Platt-Higgins, P. S. Rudland, and F. C. Campbell Ets Gene PEA3 Cooperates with {beta}-Catenin-Lef-1 and c-Jun in Regulation of Osteopontin Transcription J. Biol. Chem., May 14, 2004; 279(20): 20794 - 20806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, W. Shi, X. Ji, C. Sun, W. S. S. Jee, Y. Wu, Z. Mao, T. R. Nagy, Q. Li, and X. Cao Molecules Mimicking Smad1 Interacting with Hox Stimulate Bone Formation J. Biol. Chem., March 19, 2004; 279(12): 11313 - 11319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Hyman, L. Bartholin, S. J. Newfeld, and D. Wotton Drosophila TGIF Proteins Are Transcriptional Activators Mol. Cell. Biol., December 15, 2003; 23(24): 9262 - 9274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Grienenberger, S. Merabet, J. Manak, I. Iltis, A. Fabre, H. Berenger, M. P. Scott, J. Pradel, and Y. Graba Tgf{beta} signaling acts on a Hox response element to confer specificity and diversity to Hox protein function Development, November 15, 2003; 130(22): 5445 - 5455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Suzuki, N. Ueno, and A. Kuroiwa Hox Proteins Functionally Cooperate with the GC Box-binding Protein System through Distinct Domains J. Biol. Chem., August 8, 2003; 278(32): 30148 - 30156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Luo Negative Regulation of BMP Signaling by the Ski Oncoprotein J. Bone Joint Surg. Am., August 1, 2003; 85(90003): 39 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bidder, J.-S. Shao, N. Charlton-Kachigian, A. P. Loewy, C. F. Semenkovich, and D. A. Towler Osteopontin Transcription in Aortic Vascular Smooth Muscle Cells Is Controlled by Glucose-regulated Upstream Stimulatory Factor and Activator Protein-1 Activities J. Biol. Chem., November 8, 2002; 277(46): 44485 - 44496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-F. Lai and S.-L. Cheng Signal Transductions Induced by Bone Morphogenetic Protein-2 and Transforming Growth Factor-beta in Normal Human Osteoblastic Cells J. Biol. Chem., May 3, 2002; 277(18): 15514 - 15522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bai and X. Cao A Nuclear Antagonistic Mechanism of Inhibitory Smads in Transforming Growth Factor-beta Signaling J. Biol. Chem., February 1, 2002; 277(6): 4176 - 4182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. T. Park and M. I. Morasso Bone morphogenetic protein-2 (BMP-2) transactivates Dlx3 through Smad1 and Smad4: alternative mode for Dlx3 induction in mouse keratinocytes Nucleic Acids Res., January 15, 2002; 30(2): 515 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. ZAFFRAN, X. XU, P.C.H. LO, H.-H. LEE, and M. FRASCH Cardiogenesis in the Drosophila Model: Control Mechanisms during Early Induction and Diversification of Cardiac Progenitors Cold Spring Harb Symp Quant Biol, January 1, 2002; 67(0): 1 - 12. [Abstract] [PDF]
|
|
|
|
 |
|
 L. You and F. E. Kruse Differential Effect of Activin A and BMP-7 on Myofibroblast Differentiation and the Role of the Smad Signaling Pathway Invest. Ophthalmol. Vis. Sci., January 1, 2002; 43(1): 72 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Leboy, G. Grasso-Knight, M. D'Angelo, S. W. Volk, J. B. Lian, H. Drissi, G. S. Stein, and S. L. Adams Smad-Runx Interactions During Chondrocyte Maturation J. Bone Joint Surg. Am., April 1, 2001; 83(90010): S15 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Leboy, G. Grasso-Knight, M. D'Angelo, S. W. Volk, J. B. Lian, H. Drissi, G. S. Stein, and S. L. Adams Smad-Runx Interactions During Chondrocyte Maturation J. Bone Joint Surg. Am., March 1, 2001; 83(1_suppl_1): S15 - S22. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Torres-Vazquez, S Park, R Warrior, and K Arora The transcription factor Schnurri plays a dual role in mediating Dpp signaling during embryogenesis Development, January 5, 2001; 128(9): 1657 - 1670. [Abstract] [PDF]
|
|
|
|
|
|
S. Bai, X. Shi, X. Yang, and X. Cao Smad6 as a Transcriptional Corepressor J. Biol. Chem., March 17, 2000; 275(12): 8267 - 8270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wan, X. Shi, X. Feng, and X. Cao Transcriptional Mechanisms of Bone Morphogenetic Protein-induced Osteoprotegrin Gene Expression J. Biol. Chem., March 23, 2001; 276(13): 10119 - 10125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Mikkola, J.-A. Bruun, T. Holm, and T. Johansen Superactivation of Pax6-mediated Transactivation from Paired Domain-binding Sites by DNA-independent Recruitment of Different Homeodomain Proteins J. Biol. Chem., February 2, 2001; 276(6): 4109 - 4118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Henningfeld, H. Friedle, S. Rastegar, and W. Knochel Autoregulation of Xvent-2B; Direct Interaction and Functional Cooperation of Xvent-2 and Smad1 J. Biol. Chem., January 11, 2002; 277(3): 2097 - 2103. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
