| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 2, 1279-1286, January 14, 2000
From the Faculty of Integrated Arts and Sciences, Tokushima
University, Tokushima 770-8502, Japan
A novel eosinophil chemotactic cytokine (ECF-L)
was purified from the culture supernatant of splenocytes of mice by a
combination of anion-exchange chromatography, Procion red-agarose
affinity chromatography, size exclusion high performance liquid
chromatography (HPLC), and reverse phase HPLC. The
NH2-terminal amino acid sequence was determined by
direct protein sequencing. An ECF-L cDNA clone of 1,506 nucleotides
was isolated from a cDNA library, and the nucleotide sequence
predicted a mature protein of 397 amino acids. A recombinant ECF-L
showed a level of eosinophil chemotactic activity comparable with that
of natural ECF-L, and the activity was inhibited by a monoclonal
antibody to ECF-L. ECF-L also attracted T lymphocytes and bone marrow
polymorphonuclear leukocytes in vitro, whereas it caused
selective extravasation of eosinophils in vivo. ECF-L mRNA was highly expressed in spleen, bone marrow, lung, and heart. A comprehensive GenBank data base search revealed that ECF-L is a
chitinase family protein. ECF-L retains those amino acids highly conserved among chitinase family proteins, but Asp and Glu residues essential for the proton donation in hydrolysis were replaced by Asn
and Gln, respectively. Although ECF-L contains a consensus CXC sequence near the NH2 terminus akin to
chemokine family proteins, the rest of ECF-L shows poor homology with chemokines.
The nucleotide sequence reported in this paper has been submitted
to the DDBJ/GenBankTM/EBI Data Bank with accession number
D87757.
Identification of a Novel Eosinophil Chemotactic Cytokine
(ECF-L) as a Chitinase Family Protein*
,
*
This work was supported in part by Grants 06670260, 09309010, and 10670229 from the Ministry of Education, Science, Sports, and Culture, Japan.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 88-656-7261; Fax:
88-656-7298; E-mail: ohashi@ias.tokushima-u.ac.jp.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. G. Nair, K. J. Guild, and D. Artis Novel Effector Molecules in Type 2 Inflammation: Lessons Drawn from Helminth Infection and Allergy J. Immunol., August 1, 2006; 177(3): 1393 - 1399. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Iwashita, S. Morita, Y. Sagiya, and A. Nakanishi Role of Eosinophil Chemotactic Factor by T Lymphocytes on Airway Hyperresponsiveness in a Murine Model of Allergic Asthma Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 103 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Taylor, A. Harris, M. G. Nair, R. M. Maizels, and J. E. Allen F4/80+ Alternatively Activated Macrophages Control CD4+ T Cell Hyporesponsiveness at Sites Peripheral to Filarial Infection. J. Immunol., June 1, 2006; 176(11): 6918 - 6927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kzhyshkowska, S. Mamidi, A. Gratchev, E. Kremmer, C. Schmuttermaier, L. Krusell, G. Haus, J. Utikal, K. Schledzewski, J. Scholtze, et al. Novel stabilin-1 interacting chitinase-like protein (SI-CLP) is up-regulated in alternatively activated macrophages and secreted via lysosomal pathway Blood, April 15, 2006; 107(8): 3221 - 3228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Johansen, B. V. Jensen, A. Roslind, D. Nielsen, and P. A. Price Serum YKL-40, A New Prognostic Biomarker in Cancer Patients? Cancer Epidemiol. Biomarkers Prev., February 1, 2006; 15(2): 194 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Greene, M. S. Rutherford, R. R. Regal, G. H. Flickinger, J. A. Hendrickson, C. Giulivi, M. E. Mohrman, D. G. Fraser, and J. F. Regal Arginase Activity Differs with Allergen in the Effector Phase of Ovalbumin- versus Trimellitic Anhydride-Induced Asthma Toxicol. Sci., December 1, 2005; 88(2): 420 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Nair, I. J. Gallagher, M. D. Taylor, P. Loke, P. S. Coulson, R. A. Wilson, R. M. Maizels, and J. E. Allen Chitinase and Fizz Family Members Are a Generalized Feature of Nematode Infection with Selective Upregulation of Ym1 and Fizz1 by Antigen-Presenting Cells Infect. Immun., January 1, 2005; 73(1): 385 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Houston, A. D. Recklies, J. C. Krupa, and D. M. F. van Aalten Structure and Ligand-induced Conformational Change of the 39-kDa Glycoprotein from Human Articular Chondrocytes J. Biol. Chem., August 8, 2003; 278(32): 30206 - 30212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. You, X. Xuan, N. Tsuji, T. Kamio, D. Taylor, N. Suzuki, and K. Fujisaki Identification and Molecular Characterization of a Chitinase from the Hard Tick Haemaphysalis longicornis J. Biol. Chem., February 28, 2003; 278(10): 8556 - 8563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Welch, L. Escoubet-Lozach, D. B. Sykes, K. Liddiard, D. R. Greaves, and C. K. Glass TH2 Cytokines and Allergic Challenge Induce Ym1 Expression in Macrophages by a STAT6-dependent Mechanism J. Biol. Chem., November 1, 2002; 277(45): 42821 - 42829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ostergaard, J. S. Johansen, T. Benfield, P. A. Price, and J. D. Lundgren YKL-40 Is Elevated in Cerebrospinal Fluid from Patients with Purulent Meningitis Clin. Vaccine Immunol., May 1, 2002; 9(3): 598 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. F. Varela, A. S. Llera, R. A. Mariuzza, and J. Tormo Crystal Structure of Imaginal Disc Growth Factor-2. A MEMBER OF A NEW FAMILY OF GROWTH-PROMOTING GLYCOPROTEINS FROM DROSOPHILA MELANOGASTER J. Biol. Chem., April 5, 2002; 277(15): 13229 - 13236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Raes, P. De Baetselier, W. Noel, A. Beschin, F. Brombacher, and G. Hassanzadeh Gh. Differential expression of FIZZ1 and Ym1 in alternatively versus classically activated macrophages J. Leukoc. Biol., April 1, 2002; 71(4): 597 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Harbord, M. Novelli, B. Canas, D. Power, C. Davis, J. Godovac-Zimmermann, J. Roes, and A. W. Segal Ym1 Is a Neutrophil Granule Protein That Crystallizes in p47phox-deficient Mice J. Biol. Chem., February 8, 2002; 277(7): 5468 - 5475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Webb, A. N. J. McKenzie, and P. S. Foster Expression of the Ym2 Lectin-binding Protein Is Dependent on Interleukin (IL)-4 and IL-13 Signal Transduction. IDENTIFICATION OF A NOVEL ALLERGY-ASSOCIATED PROTEIN J. Biol. Chem., November 2, 2001; 276(45): 41969 - 41976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wu, G. Egerton, A. P. Underwood, S. Sakuda, and A. E. Bianco Expression and Secretion of a Larval-specific Chitinase (Family 18 Glycosyl Hydrolase) by the Infective Stages of the Parasitic Nematode, Onchocerca volvulus J. Biol. Chem., November 2, 2001; 276(45): 42557 - 42564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. H. Falcone, P'n. Loke, X. Zang, A. S. MacDonald, R. M. Maizels, and J. E. Allen A Brugia malayi Homolog of Macrophage Migration Inhibitory Factor Reveals an Important Link Between Macrophages and Eosinophil Recruitment During Nematode Infection J. Immunol., November 1, 2001; 167(9): 5348 - 5354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Ward, M. Yoon, M. R. Anver, D. C. Haines, G. Kudo, F. J. Gonzalez, and S. Kimura Hyalinosis and Ym1/Ym2 Gene Expression in the Stomach and Respiratory Tract of 129S4/SvJae and Wild-Type and CYP1A2-Null B6,129 Mice Am. J. Pathol., January 1, 2001; 158(1): 323 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Boot, E. F. C. Blommaart, E. Swart, K. Ghauharali-van der Vlugt, N. Bijl, C. Moe, A. Place, and J. M. F. G. Aerts Identification of a Novel Acidic Mammalian Chitinase Distinct from Chitotriosidase J. Biol. Chem., February 23, 2001; 276(9): 6770 - 6778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N.-C. A. Chang, S.-I. Hung, K.-Y. Hwa, I. Kato, J.-E. Chen, C.-H. Liu, and A. C. Chang A Macrophage Protein, Ym1, Transiently Expressed during Inflammation Is a Novel Mammalian Lectin J. Biol. Chem., May 11, 2001; 276(20): 17497 - 17506. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
