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J Biol Chem, Vol. 275, Issue 2, 840-846, January 14, 2000
From the Ionotropic
GABA Receptor
1 Subunit Interacts with a Novel Splice
Variant of the Glycine Transporter, GLYT-1*
§,
, and**
Laboratory for Molecular Cell Biology and
Department of Pharmacology, University College London, Gower St.,
London, WC1E 6BT, United Kingdom and the ¶ Committee on
Neurobiology, The University of Chicago, Chicago, Illinois
-aminobutyric acid
(GABAA and GABAC) receptors mediate fast
synaptic inhibition in the central nervous system. GABAC
receptors are expressed predominantly in the retina on bipolar cell
axon terminals, and are thought to mediate feedback inhibition from
GABAergic amacrine cells. Utilizing the yeast two-hybrid system, we
previously identified MAP1B as a binding partner of the
GABAC receptor 
1 subunit. Here we describe the isolation of an additional 1 interacting protein: a novel C-terminal variant of the glycine transporter GLYT-1. We show that GLYT-1 exists as four
alternatively spliced mRNAs which encode proteins expressing one of
two possible intracellullar N- and C-terminal domains. Variants
containing the novel C terminus efficiently transport glycine when
expressed in COS cells, but with unusual kinetics. We have confirmed
the interaction between the novel C terminus and 1 subunit and
demonstrated binding in heterologous cells. This interaction may be
crucial for the integration of GABAergic and glycinergic
neurotransmission in the retina.
*
This work was supported by the Medical Research Council, the
Wellcome Trust, and the Royal Society.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Dept. of Physiology, University of Wisconsin,
Madison, WI.
**
To whom correspondence should be addressed: MRC-LMCB, University
College London, Gower St., London, WC1E 6BT United Kingdom. Fax.:
44-171-380-7805; E-mail: steve.moss@ucl.ac.uk.
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