HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Floyd, H. Articles by Crocker, P. R. Search for Related Content PubMed PubMed Citation Articles by Floyd, H. Articles by Crocker, P. R. Social Bookmarking                      What's this?

J Biol Chem, Vol. 275, Issue 2, 861-866, January 14, 2000

Siglec-8
A NOVEL EOSINOPHIL-SPECIFIC MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY^*

Helen Floyd, Jian Ni^§, Ann L. Cornish, Zhizhen Zeng^§, Ding Liu^§, Kenneth C. Carter^§, Jane Steel^¶, and Paul R. Crocker

From  The Wellcome Trust Biocentre at Dundee, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom, ^§ Human Genome Sciences, Inc., Rockville, Maryland 20850-3338, and the ^¶ Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC1, United Kingdom

We describe the characterization of siglec-8, a novel sialic acid-binding immunoglobulin-like lectin that is expressed specifically by eosinophils. A full-length cDNA encoding siglec-8 was isolated from a human eosinophil cDNA library. Siglec-8 is predicted to contain three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail of 47 amino acids. The siglec-8 gene mapped on chromosome 19q13.33-41, closely linked to genes encoding CD33 (siglec-3), siglec-5, siglec-6, and siglec-7. When siglec-8 was expressed on COS cells or as a recombinant protein fused to the Fc region of human IgG₁, it was able to mediate sialic acid-dependent binding to human erythrocytes and to soluble sialoglycoconjugates. Using specific monoclonal antibodies, siglec-8 could be detected only on eosinophils and hence appears to be the first example of an eosinophil-specific transmembrane receptor.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. Zhang, T. Angata, J. Y. Cho, M. Miller, D. H. Broide, and A. Varki Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils Blood, May 15, 2007; 109(10): 4280 - 4287. [Abstract] [Full Text] [PDF]

				A. F. Carlin, A. L. Lewis, A. Varki, and V. Nizet Group B Streptococcal Capsular Sialic Acids Interact with Siglecs (Immunoglobulin-Like Lectins) on Human Leukocytes J. Bacteriol., February 15, 2007; 189(4): 1231 - 1237. [Abstract] [Full Text] [PDF]

				J. Zhang, A. Raper, N. Sugita, R. Hingorani, M. Salio, M. J. Palmowski, V. Cerundolo, and P. R. Crocker Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors Blood, May 1, 2006; 107(9): 3600 - 3608. [Abstract] [Full Text] [PDF]

				A. Varki and T. Angata Siglecs--the major subfamily of I-type lectins Glycobiology, January 1, 2006; 16(1): 1R - 27R. [Abstract] [Full Text] [PDF]

				H. Tateno, P. R. Crocker, and J. C. Paulson Mouse Siglec-F and human Siglec-8 are functionally convergent paralogs that are selectively expressed on eosinophils and recognize 6'-sulfo-sialyl Lewis X as a preferred glycan ligand Glycobiology, November 1, 2005; 15(11): 1125 - 1135. [Abstract] [Full Text] [PDF]

				B. S. Bochner, R. A. Alvarez, P. Mehta, N. V. Bovin, O. Blixt, J. R. White, and R. L. Schnaar Glycan Array Screening Reveals a Candidate Ligand for Siglec-8 J. Biol. Chem., February 11, 2005; 280(6): 4307 - 4312. [Abstract] [Full Text] [PDF]

				T. Avril, H. Floyd, F. Lopez, E. Vivier, and P. R. Crocker The Membrane-Proximal Immunoreceptor Tyrosine-Based Inhibitory Motif Is Critical for the Inhibitory Signaling Mediated by Siglecs-7 and -9, CD33-Related Siglecs Expressed on Human Monocytes and NK Cells J. Immunol., December 1, 2004; 173(11): 6841 - 6849. [Abstract] [Full Text] [PDF]

				O. Blixt, B. E. Collins, I. M. van den Nieuwenhof, P. R. Crocker, and J. C. Paulson Sialoside Specificity of the Siglec Family Assessed Using Novel Multivalent Probes: IDENTIFICATION OF POTENT INHIBITORS OF MYELIN-ASSOCIATED GLYCOPROTEIN J. Biol. Chem., August 15, 2003; 278(33): 31007 - 31019. [Abstract] [Full Text] [PDF]

				E. C. M. Brinkman-Van der Linden, T. Angata, S. A. Reynolds, L. D. Powell, S. M. Hedrick, and A. Varki CD33/Siglec-3 Binding Specificity, Expression Pattern, and Consequences of Gene Deletion in Mice Mol. Cell. Biol., June 15, 2003; 23(12): 4199 - 4206. [Abstract] [Full Text] [PDF]

				E. Nutku, H. Aizawa, S. A. Hudson, and B. S. Bochner Ligation of Siglec-8: a selective mechanism for induction of human eosinophil apoptosis Blood, June 15, 2003; 101(12): 5014 - 5020. [Abstract] [Full Text] [PDF]

				B. E. Collins, O. Blixt, N. V. Bovin, C.-P. Danzer, D. Chui, J. D. Marth, L. Nitschke, and J. C. Paulson Constitutively unmasked CD22 on B cells of ST6Gal I knockout mice: novel sialoside probe for murine CD22 Glycobiology, September 1, 2002; 12(9): 563 - 571. [Abstract] [Full Text] [PDF]

				T. Angata, S. C. Kerr, D. R. Greaves, N. M. Varki, P. R. Crocker, and A. Varki Cloning and Characterization of Human Siglec-11. A RECENTLY EVOLVED SIGNALING MOLECULE THAT CAN INTERACT WITH SHP-1 AND SHP-2 AND IS EXPRESSED BY TISSUE MACROPHAGES, INCLUDING BRAIN MICROGLIA J. Biol. Chem., June 28, 2002; 277(27): 24466 - 24474. [Abstract] [Full Text] [PDF]

				K. Grobe and L. D. Powell Role of protein kinase C in the phosphorylation of CD33 (Siglec-3) and its effect on lectin activity Blood, May 1, 2002; 99(9): 3188 - 3196. [Abstract] [Full Text] [PDF]

				T. K. van den Berg, D. Nath, H. J. Ziltener, D. Vestweber, M. Fukuda, I. van Die, and P. R. Crocker Cutting Edge: CD43 Functions as a T Cell Counterreceptor for the Macrophage Adhesion Receptor Sialoadhesin (Siglec-1) J. Immunol., March 15, 2001; 166(6): 3637 - 3640. [Abstract] [Full Text] [PDF]

				N. Scholler, M. Hayden-Ledbetter, K.-E. Hellstrom, I. Hellstrom, and J. A. Ledbetter CD83 Is a Sialic Acid-Binding Ig-Like Lectin (Siglec) Adhesion Receptor that Binds Monocytes and a Subset of Activated CD8+ T Cells J. Immunol., March 15, 2001; 166(6): 3865 - 3872. [Abstract] [Full Text] [PDF]

				A. Hartnell, J. Steel, H. Turley, M. Jones, D. G. Jackson, and P. R. Crocker Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations Blood, January 1, 2001; 97(1): 288 - 296. [Abstract] [Full Text] [PDF]

				S. P. Paul, L. S. Taylor, E. K. Stansbury, and D. W. McVicar Myeloid specific human CD33 is an inhibitory receptor with differential ITIM function in recruiting the phosphatases SHP-1 and SHP-2 Blood, July 15, 2000; 96(2): 483 - 490. [Abstract] [Full Text] [PDF]

				T. Angata and A. Varki Cloning, Characterization, and Phylogenetic Analysis of Siglec-9, a New Member of the CD33-related Group of Siglecs. EVIDENCE FOR CO-EVOLUTION WITH SIALIC ACID SYNTHESIS PATHWAYS J. Biol. Chem., July 14, 2000; 275(29): 22127 - 22135. [Abstract] [Full Text] [PDF]

				J. Q. Zhang, G. Nicoll, C. Jones, and P. R. Crocker Siglec-9, a Novel Sialic Acid Binding Member of the Immunoglobulin Superfamily Expressed Broadly on Human Blood Leukocytes J. Biol. Chem., July 14, 2000; 275(29): 22121 - 22126. [Abstract] [Full Text] [PDF]

				T. Ulyanova, D. D. Shah, and M. L. Thomas Molecular Cloning of MIS, a Myeloid Inhibitory Siglec, That Binds Protein-tyrosine Phosphatases SHP-1 and SHP-2 J. Biol. Chem., April 20, 2001; 276(17): 14451 - 14458. [Abstract] [Full Text] [PDF]

				M. Vinson, P. J. L. M. Strijbos, A. Rowles, L. Facci, S. E. Moore, D. L. Simmons, and F. S. Walsh Myelin-associated Glycoprotein Interacts with Ganglioside GT1b. A MECHANISM FOR NEURITE OUTGROWTH INHIBITION J. Biol. Chem., June 1, 2001; 276(23): 20280 - 20285. [Abstract] [Full Text] [PDF]

				N. Li, W. Zhang, T. Wan, J. Zhang, T. Chen, Y. Yu, J. Wang, and X. Cao Cloning and Characterization of Siglec-10, a Novel Sialic Acid Binding Member of the Ig Superfamily, from Human Dendritic Cells J. Biol. Chem., July 20, 2001; 276(30): 28106 - 28112. [Abstract] [Full Text] [PDF]

				Z. Yu, C.-M. Lai, M. Maoui, D. Banville, and S.-H. Shen Identification and Characterization of S2V, a Novel Putative Siglec That Contains Two V Set Ig-like Domains and Recruits Protein-tyrosine Phosphatases SHPs J. Biol. Chem., June 22, 2001; 276(26): 23816 - 23824. [Abstract] [Full Text] [PDF]