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J Biol Chem, Vol. 275, Issue 20, 14791-14794, May 19, 2000

ACCELERATED PUBLICATION
Insertional Mutation of the Murine Kisimo Locus Caused a Defect in Spermatogenesis^*

Noriyuki Yanaka, Kinji Kobayashi^§, Koji Wakimoto^¶, Eriko Yamada^¶, Hiroshi Imahie^§, Yuji Imai^¶, and Chisato Mori

From the Discovery Research Laboratory, ^§ Safety Research Laboratory, and ^¶ Department of Advanced Medical Research, Tanabe Seiyaku Co., Ltd., 16-89, Kashima 3-chome, Yodogawa-ku, Osaka 532-8505 and the  Department of Anatomy, Faculty of Medicine, Kyoto University, Konoe-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan

Spermatogenesis is a developmental process that occurs in several phases and is regulated by a large number of gene products. An insertional transgenic mouse mutant (termed kisimo mouse) has been isolated that results in abnormal germ-cell development, showing abnormal elongated spermatids in the lumina of seminiferous tubules. We cloned the disrupted locus of kisimo and identified a novel testis-specific gene, THEG, which is specifically expressed in spermatids and was disrupted in the transgenic mouse. The yeast two-hybrid screening method revealed that THEG protein strongly interacts with chaperonin containing t-complex polypeptide-1, suggesting that THEG protein functions as a regulatory factor in protein assembly. Our findings indicate that the kisimo locus is essential for the maintenance of spermiogenesis and that a gene expression disorder may be involved in male infertility.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AB033128 and AB033129.

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