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Originally published In Press as doi:10.1074/jbc.C901047199 on March 20, 2000
J Biol Chem, Vol. 275, Issue 20, 14791-14794, May 19, 2000
ACCELERATED PUBLICATION
Insertional Mutation of the Murine Kisimo Locus Caused a Defect
in Spermatogenesis*
Noriyuki
Yanaka ,
Kinji
Kobayashi§,
Koji
Wakimoto¶,
Eriko
Yamada¶,
Hiroshi
Imahie§,
Yuji
Imai¶, and
Chisato
Mori
From the Discovery Research Laboratory, § Safety
Research Laboratory, and ¶ Department of Advanced Medical
Research, Tanabe Seiyaku Co., Ltd., 16-89, Kashima 3-chome,
Yodogawa-ku, Osaka 532-8505 and the Department of Anatomy,
Faculty of Medicine, Kyoto University, Konoe-cho, Yoshida, Sakyo-ku,
Kyoto 606-8501, Japan
Spermatogenesis is a developmental process that
occurs in several phases and is regulated by a large number of gene
products. An insertional transgenic mouse mutant (termed kisimo mouse)
has been isolated that results in abnormal germ-cell development, showing abnormal elongated spermatids in the lumina of seminiferous tubules. We cloned the disrupted locus of kisimo and identified a novel
testis-specific gene, THEG, which is specifically expressed in spermatids and was disrupted in the transgenic mouse. The yeast two-hybrid screening method revealed that THEG protein strongly interacts with chaperonin containing t-complex
polypeptide-1 , suggesting that THEG protein functions as a
regulatory factor in protein assembly. Our findings indicate that the
kisimo locus is essential for the maintenance of spermiogenesis and
that a gene expression disorder may be involved in male infertility.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AB033128 and AB033129.
To whom correspondence should be addressed. Tel.: 81-6-6300-2577;
Fax: 81-6-6300-2593; E-mail: n-yanaka@tanabe.co.jp.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.

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Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
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