| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 20, 14853-14859, May 19, 2000
From the Laminins are a large family of heterotrimeric
extracellular matrix glycoproteins that, in addition to having
structural roles, take part in the regulation of processes such as cell
migration, differentiation, and proliferation. The laminin
Recombinant Laminin-8
(
4
1
1)
PRODUCTION, PURIFICATION, AND INTERACTIONS WITH INTEGRINS*
,¶
Division of Matrix Biology, Department of
Medical Biochemistry and Biophysics, Karolinska Institutet, S-17177
Stockholm, Sweden and the § Department of Pathology, Robert
Wood Johnson Medical School, Piscataway, New Jersey 08854
4 chain is widely distributed both in adults and
during development in tissues such as cardiac, skeletal and smooth
muscle fibers, vascular endothelia, lungs, and in peripheral nerves. It
can associate with laminin 
1/
1 chains to
form laminin-8 and with the 2/1 chains to
form laminin-9. Functional studies on these laminins have been hampered by poor availability of the protein in pure and soluble forms. To
facilitate studies on laminin-8, recombinant laminin-8 was produced in
a mammalian expression system, purified and shown to form native
Y-shaped molecules in rotary shadowing electron microscopy. Integrins
mediating cell adhesion to laminin-8 were identified using
function-blocking mAbs. The integrin specificities were found to differ
somewhat from that of laminin-1. Integrin 61 was found to be a major mediator of
adhesion of HT-1080 and cultured capillary endothelial cells to
laminin-8. Considering the expression patterns of laminin-8 and
integrin 61 it is likely that the former
is a ligand for the latter in vivo as well.
*
This work was supported by grants from Biostratum Inc., the
Novo Nordisc Foundation, the Swedish Medical Research Council, and
Hedlund's Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Domogatskaya, S. Rodin, A. Boutaud, and K. Tryggvason Laminin-511 but Not -332, -111, or -411 Enables Mouse Embryonic Stem Cell Self-Renewal In Vitro Stem Cells, November 1, 2008; 26(11): 2800 - 2809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gorfu, I. Virtanen, M. Hukkanen, V.-P. Lehto, P. Rousselle, E. Kenne, L. Lindbom, R. Kramer, K. Tryggvason, and M. Patarroyo Laminin isoforms of lymph nodes and predominant role of {alpha}5-laminin(s) in adhesion and migration of blood lymphocytes J. Leukoc. Biol., September 1, 2008; 84(3): 701 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Jakobsson, A. Domogatskaya, K. Tryggvason, D. Edgar, and L. Claesson-Welsh Laminin deposition is dispensable for vasculogenesis but regulates blood vessel diameter independent of flow FASEB J, May 1, 2008; 22(5): 1530 - 1539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Qian, E. Georges-Labouesse, A. Nystrom, A. Domogatskaya, K. Tryggvason, S. E. W. Jacobsen, and M. Ekblom Distinct roles of integrins {alpha}6 and {alpha}4 in homing of fetal liver hematopoietic stem and progenitor cells Blood, October 1, 2007; 110(7): 2399 - 2407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Meinen, P. Barzaghi, S. Lin, H. Lochmuller, and M. A. Ruegg Linker molecules between laminins and dystroglycan ameliorate laminin-{alpha}2-deficient muscular dystrophy at all disease stages J. Cell Biol., March 26, 2007; 176(7): 979 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Berfield, K. M. Hansen, and C. K. Abrass Rat glomerular mesangial cells require laminin-9 to migrate in response to insulin-like growth factor binding protein-5 Am J Physiol Cell Physiol, October 1, 2006; 291(4): C589 - C599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Qian, K. Tryggvason, S. E. Jacobsen, and M. Ekblom Contribution of {alpha}6 integrins to hematopoietic stem and progenitor cell homing to bone marrow and collaboration with {alpha}4 integrins Blood, May 1, 2006; 107(9): 3503 - 3510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, M. Hoshijima, J. Lam, Z. Zhou, A. Jokiel, N. D. Dalton, K. Hultenby, P. Ruiz-Lozano, J. Ross Jr., K. Tryggvason, et al. Cardiomyopathy Associated with Microcirculation Dysfunction in Laminin {alpha}4 Chain-deficient Mice J. Biol. Chem., January 6, 2006; 281(1): 213 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hallmann, N. Horn, M. Selg, O. Wendler, F. Pausch, and L. M. Sorokin Expression and Function of Laminins in the Embryonic and Mature Vasculature Physiol Rev, July 1, 2005; 85(3): 979 - 1000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wallquist, S. Plantman, S. Thams, J. Thyboll, J. Kortesmaa, J. Lannergren, A. Domogatskaya, S. O. Ogren, M. Risling, H. Hammarberg, et al. Impeded Interaction between Schwann Cells and Axons in the Absence of Laminin {alpha}4 J. Neurosci., April 6, 2005; 25(14): 3692 - 3700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-J. Leu, N. Chen, C.-C. Chen, V. Todorovic, T. Bai, V. Juric, Y. Liu, G. Yan, S. C.-T. Lam, and L. F. Lau Targeted Mutagenesis of the Angiogenic Protein CCN1 (CYR61): SELECTIVE INACTIVATION OF INTEGRIN {alpha}6{beta}1-HEPARAN SULFATE PROTEOGLYCAN CORECEPTOR-MEDIATED CELLULAR FUNCTIONS J. Biol. Chem., October 15, 2004; 279(42): 44177 - 44187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wondimu, T. Geberhiwot, S. Ingerpuu, E. Juronen, X. Xie, L. Lindbom, M. Doi, J. Kortesmaa, J. Thyboll, K. Tryggvason, et al. An endothelial laminin isoform, laminin 8 ({alpha}4{beta}1{gamma}1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis Blood, September 15, 2004; 104(6): 1859 - 1866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Calzada, J. M. Sipes, H. C. Krutzsch, P. D. Yurchenco, D. S. Annis, D. F. Mosher, and D. D. Roberts Recognition of the N-terminal Modules of Thrombospondin-1 and Thrombospondin-2 by {alpha}6{beta}1 Integrin J. Biol. Chem., October 17, 2003; 278(42): 40679 - 40687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Nishiuchi, O. Murayama, H. Fujiwara, J. Gu, T. Kawakami, S. Aimoto, Y. Wada, and K. Sekiguchi Characterization of the Ligand-Binding Specificities of Integrin {alpha}3{beta}1 and {alpha}6{beta}1 Using a Panel of Purified Laminin Isoforms Containing Distinct {alpha} Chains J. Biochem., October 1, 2003; 134(4): 497 - 504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Gu, J. Kortesmaa, K. Tryggvason, J. Persson, P. Ekblom, S.-E. Jacobsen, and M. Ekblom Laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells Blood, February 1, 2003; 101(3): 877 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Okazaki, N. Suzuki, N. Nishi, A. Utani, H. Matsuura, H. Shinkai, H. Yamashita, Y. Kitagawa, and M. Nomizu Identification of Biologically Active Sequences in the Laminin alpha 4 Chain G Domain J. Biol. Chem., September 27, 2002; 277(40): 37070 - 37078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Petajaniemi, M. Korhonen, J. Kortesmaa, K. Tryggvason, K. Sekiguchi, H. Fujiwara, L. Sorokin, L.-E. Thornell, Z. Wondimu, D. Assefa, et al. Localization of Laminin {alpha}4-Chain in Developing and Adult Human Tissues J. Histochem. Cytochem., August 1, 2002; 50(8): 1113 - 1130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Li, D. Harrison, S. Carbonetto, R. Fassler, N. Smyth, D. Edgar, and P. D. Yurchenco Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation J. Cell Biol., June 24, 2002; 157(7): 1279 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Doi, J. Thyboll, J. Kortesmaa, K. Jansson, A. Iivanainen, M. Parvardeh, R. Timpl, U. Hedin, J. Swedenborg, and K. Tryggvason Recombinant Human Laminin-10 (alpha 5beta 1gamma 1). PRODUCTION, PURIFICATION, AND MIGRATION-PROMOTING ACTIVITY ON VASCULAR ENDOTHELIAL CELLS J. Biol. Chem., April 5, 2002; 277(15): 12741 - 12748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Thyboll, J. Kortesmaa, R. Cao, R. Soininen, L. Wang, A. Iivanainen, L. Sorokin, M. Risling, Y. Cao, and K. Tryggvason Deletion of the Laminin {alpha}4 Chain Leads to Impaired Microvessel Maturation Mol. Cell. Biol., February 15, 2002; 22(4): 1194 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. P. Moiseeva Adhesion receptors of vascular smooth muscle cells and their functions Cardiovasc Res, December 1, 2001; 52(3): 372 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sixt, B. Engelhardt, F. Pausch, R. Hallmann, O. Wendler, and L. M. Sorokin Endothelial Cell Laminin Isoforms, Laminins 8 and 10, Play Decisive Roles in T Cell Recruitment Across the Blood-Brain Barrier in Experimental Autoimmune Encephalomyelitis J. Cell Biol., May 21, 2001; 153(5): 933 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Bell, A. Mavila, R. Salazar, K. J. Bayless, S. Kanagala, S. A. Maxwell, and G. E. Davis Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling J. Cell Sci., January 8, 2001; 114(15): 2755 - 2773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Spessotto, Z. Yin, G. Magro, R. Deutzmann, A. Chiu, A. Colombatti, and R. Perris Laminin Isoforms 8 and 10 Are Primary Components of the Subendothelial Basement Membrane Promoting Interaction with Neoplastic Lymphocytes Cancer Res., January 1, 2001; 61(1): 339 - 347. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Gonzales, B. Weksler, D. Tsuruta, R. D. Goldman, K. J. Yoon, S. B. Hopkinson, F. W. Flitney, and J. C. R. Jones Structure and Function of a Vimentin-associated Matrix Adhesion in Endothelial Cells Mol. Biol. Cell, January 1, 2001; 12(1): 85 - 100. [Abstract] [Full Text]
|
|
|
|
|
|
U. Siler, M. Seiffert, S. Puch, A. Richards, B. Torok-Storb, C. A. Muller, L. Sorokin, and G. Klein Characterization and functional analysis of laminin isoforms in human bone marrow Blood, December 15, 2000; 96(13): 4194 - 4203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Talts, T. Sasaki, N. Miosge, W. Gohring, K. Mann, R. Mayne, and R. Timpl Structural and Functional Analysis of the Recombinant G Domain of the Laminin alpha 4 Chain and Its Proteolytic Processing in Tissues J. Biol. Chem., November 3, 2000; 275(45): 35192 - 35199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujiwara, Y. Kikkawa, N. Sanzen, and K. Sekiguchi Purification and Characterization of Human Laminin-8. LAMININ-8 STIMULATES CELL ADHESION AND MIGRATION THROUGH alpha 3beta 1 AND alpha 6beta 1 INTEGRINS J. Biol. Chem., May 11, 2001; 276(20): 17550 - 17558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sixt, R. Hallmann, O. Wendler, K. Scharffetter-Kochanek, and L. M. Sorokin Cell Adhesion and Migration Properties of beta 2-Integrin Negative Polymorphonuclear Granulocytes on Defined Extracellular Matrix Molecules. RELEVANCE FOR LEUKOCYTE EXTRAVASATION J. Biol. Chem., May 25, 2001; 276(22): 18878 - 18887. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
