JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Razzini, G.
Right arrow Articles by Falasca, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Razzini, G.
Right arrow Articles by Falasca, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 20, 14873-14881, May 19, 2000

The Role of the Pleckstrin Homology Domain in Membrane Targeting and Activation of Phospholipase Cbeta 1*

Giorgia RazziniDagger , Anna BrancaccioDagger , Mark A. Lemmon§, Simone Guarnieri, and Marco FalascaDagger ||**

From the Dagger  Unit of Physiopathology of Cell Signalling, Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche "Mario Negri," Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro, Italy, the § Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6089, the  Laboratory of Cellular Physiology, Department of Pharmacological Science, G. D'Annunzio University, 66013 Chieti, Italy, and the || Laboratory of Molecular Oncology, Fondazione Caripe, 65100 Pescara, Italy

Current studies involve an investigation of the role of the pleckstrin homology (PH) domain in membrane targeting and activation of phospholipase Cbeta 1 (PLCbeta 1). Here we report studies on the membrane localization of the isolated PH domain from the amino terminus of PLCbeta 1 (PLCbeta 1-PH) using fluorescence microscopy of a green fluorescent protein fusion protein. Whereas PLCbeta 1-PH does not localize to the plasma membrane in serum-starved cells, it undergoes a rapid but transient migration to the plasma membrane upon stimulation of cells with serum or lysophosphatidic acid (LPA). Regulation of the plasma membrane localization of PLCbeta 1-PH by phosphoinositides was also investigated. PLCbeta 1-PH was found to bind phosphatidylinositol 3-phosphate most strongly, whereas other phosphoinositides were bound with lower affinity. The plasma membrane localization of PLCbeta 1-PH induced by serum and LPA was blocked by wortmannin pretreatment and by LY294002. In parallel, activation of PLCbeta by LPA was inhibited by wortmannin, by LY294002, or by the overexpression of PLCbeta 1-PH. Microinjection of beta gamma subunits of G proteins in serum-starved cells induced the translocation of PLCbeta 1-PH to the plasma membrane. These results demonstrate that a cooperative mechanism involving phosphatidylinositol 3-phosphate and the Gbeta gamma subunit regulates the plasma membrane localization and activation of PLCbeta 1-PH.

* This work was supported in part by Telethon, Italy, Grant 328/bi, the Lega Italiana per la Lotta Contro i Tumori, Pescara, and the Italian National Research Council (Convenzione C. N. R. Consorzio Mario Negri Sud) (to M. F.) and National Institutes of Health Grant GM56846 (to M. A. L.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

** To whom correspondence should be addressed: Unit of Physiopathology of Cell Signalling, Dept. of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro (CH), Italy. Tel.: 39 0872 570336; Fax: 39 0872 570412; E-mail: falasca@cmns.mnegri.it.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
P. J. Wen, S. L. Osborne, I. C. Morrow, R. G. Parton, J. Domin, and F. A. Meunier
Ca2+-regulated Pool of Phosphatidylinositol-3-phosphate Produced by Phosphatidylinositol 3-Kinase C2{alpha} on Neurosecretory Vesicles
Mol. Biol. Cell, December 1, 2008; 19(12): 5593 - 5603.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
Y. Naito, M. Okada, and H. Yagisawa
Phospholipase C Isoforms Are Localized at the Cleavage Furrow during Cytokinesis
J. Biochem., December 1, 2006; 140(6): 785 - 791.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Nakahara, M. Shimozawa, Y. Nakamura, Y. Irino, M. Morita, Y. Kudo, and K. Fukami
A Novel Phospholipase C, PLC{eta}2, Is a Neuron-specific Isozyme
J. Biol. Chem., August 12, 2005; 280(32): 29128 - 29134.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. Maffucci, F. T. Cooke, F. M. Foster, C. J. Traer, M. J. Fry, and M. Falasca
Class II phosphoinositide 3-kinase defines a novel signaling pathway in cell migration
J. Cell Biol., June 6, 2005; 169(5): 789 - 799.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. V. Stahelin, B. Ananthanarayanan, N. R. Blatner, S. Singh, K. S. Bruzik, D. Murray, and W. Cho
Mechanism of Membrane Binding of the Phospholipase D1 PX Domain
J. Biol. Chem., December 24, 2004; 279(52): 54918 - 54926.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Chua and V. Deretic
Mycobacterium tuberculosis Reprograms Waves of Phosphatidylinositol 3-Phosphate on Phagosomal Organelles
J. Biol. Chem., August 27, 2004; 279(35): 36982 - 36992.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Popova and M. M. Rasenick
Clathrin-mediated Endocytosis of m3 Muscarinic Receptors: ROLES FOR G{beta}{gamma} AND TUBULIN
J. Biol. Chem., July 16, 2004; 279(29): 30410 - 30418.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Popova and M. M. Rasenick
G{beta}{gamma} Mediates the Interplay between Tubulin Dimers and Microtubules in the Modulation of Gq Signaling
J. Biol. Chem., September 5, 2003; 278(36): 34299 - 34308.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
S. M. Singh and D. Murray
Molecular modeling of the membrane targeting of phospholipase C pleckstrin homology domains
Protein Sci., September 1, 2003; 12(9): 1934 - 1953.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. T. Lodowski, J. A. Pitcher, W. D. Capel, R. J. Lefkowitz, and J. J. G. Tesmer
Keeping G Proteins at Bay: A Complex Between G Protein-Coupled Receptor Kinase 2 and G{beta}{gamma}
Science, May 23, 2003; 300(5623): 1256 - 1262.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Illenberger, C. Walliser, B. Nurnberg, M. D. Lorente, and P. Gierschik
Specificity and Structural Requirements of Phospholipase C-beta Stimulation by Rho GTPases Versus G Protein beta gamma Dimers
J. Biol. Chem., January 24, 2003; 278(5): 3006 - 3014.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Saxena, P. Morozov, D. Frank, R. Musalo, M. A. Lemmon, E. Y. Skolnik, and B. Tycko
Phosphoinositide Binding by the Pleckstrin Homology Domains of Ipl and Tih1
J. Biol. Chem., December 13, 2002; 277(51): 49935 - 49944.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-S. Chang, H. Seok, T.-K. Kwon, D. S. Min, B.-H. Ahn, Y. H. Lee, J.-W. Suh, J.-W. Kim, S. Iwashita, A. Omori, et al.
Interaction of Elongation Factor-1alpha and Pleckstrin Homology Domain of Phospholipase C-gamma 1 with Activating Its Activity
J. Biol. Chem., May 24, 2002; 277(22): 19697 - 19702.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Vanni, P. Mancini, Y. Gao, C. Ottaviano, F. Guo, B. Salani, M. R. Torrisi, Y. Zheng, and A. Eva
Regulation of Proto-Dbl by Intracellular Membrane Targeting and Protein Stability
J. Biol. Chem., May 24, 2002; 277(22): 19745 - 19753.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. T. Snyder, K. L. Rossman, M. A. Baumeister, W. M. Pruitt, D. P. Siderovski, C. J. Der, M. A. Lemmon, and J. Sondek
Quantitative Analysis of the Effect of Phosphoinositide Interactions on the Function of Dbl Family Proteins
J. Biol. Chem., November 30, 2001; 276(49): 45868 - 45875.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. J. Rebecchi and S. N. Pentyala
Structure, Function, and Control of Phosphoinositide-Specific Phospholipase C
Physiol Rev, October 1, 2000; 80(4): 1291 - 1335.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.