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J Biol Chem, Vol. 275, Issue 20, 14910-14915, May 19, 2000
From the Department of Anatomy and Cell Biology, University of
Florida College of Medicine, Gainesville, Florida 32610-0235
Pinin is a cell adhesion-associated and nuclear
protein that has been shown to localize in the vicinity of intermediate
filament (IF) convergence upon the cytoplasmic face of the desmosomal
plaque as well as in the nucleus. The localization of pinin to the
desmosomes has been correlated with the reinforcement of intercellular
adhesion and increased IF organization. In this study, keratins 18, 8, and 19 were identified to interact with the amino end domain of pinin
in a two-hybrid screening. Further truncation analyses indicated that
the 2B domain of keratin contains the sequence responsible for
interacting with pinin. The amino end of pinin (residues 1-98) is
sufficient to bind to keratin. Point mutation analyses revealed two
essential residues within the pinin fragment 1-98, leucine 8 and
leucine 19, for the interaction with keratin. Finally, in vitro protein overlay binding assays confirmed the direct
interaction of the amino end domain of pinin with keratins, while pinin
mutant L8P GST fusion protein failed to bind to keratins in the overlay assay. Coupled with our previous morphological observations and transfection studies, these data suggest that pinin may play a role in
epithelial cell adhesion and the IF complex through a direct
interaction with the keratin filaments.
Dissection of Protein Linkage between Keratins and Pinin, a
Protein with Dual Location at Desmosome-Intermediate Filament
Complex and in the Nucleus*
*
This work was supported by National Institutes of Health
Grant EY07883.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Anatomy and
Cell Biology, University of Florida College of Medicine, 1600 S.W.
Archer Rd., Gainesville, FL 32610-0235. Tel.: 352-392-3569; Fax:
352-392-3305; E-mail: sugrue@anatomy.med.ufl.edu.
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