| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 20, 15279-15286, May 19, 2000
From the Lipids and other membrane constituents recycle
between the plasma membrane and intracellular endocytic compartments.
In CHO cells, approximately half of the internalized
C6-NBD-SM, a fluorescent lipid analogue widely used
as a membrane maker, recycles via the endocytic recycling compartment
with a t1/2 of ~12 min (Mayor, S., Presley,
J. F., and Maxfield, F. R. (1993) J. Cell
Biol. 121, 1257-1269). Surprisingly, the rest returns to the
plasma membrane very quickly. A detailed kinetic study presented in
this paper indicates that after a brief internalization pulse, 42-62%
of the internalized C6-NBD-SM returns to the plasma membrane with a t1/2 of 1-2 min. Similar results
are obtained using HEp2 and nonpolarized Madin-Darby canine kidney
cells. Using FM dyes of different hydrophobicity, we show that rapid
recycling involves passage through an endocytic organelle that was
subsequently identified as the sorting endosome by co-localization with
internalized transferrin and low density lipoprotein. These results
imply that the membrane internalization rate is much higher than
previously estimated, with a t1/2 as short as 5-10
min. Rapid internalization and recycling would facilitate processes
such as nutrient uptake and cholesterol efflux.
Characterization of Rapid Membrane Internalization and
Recycling*
§ and¶
Department of Biochemistry, Weill Medical
College of Cornell University, New York, New York 10021 and the
§ Department of Chemistry and Chemical Biology, Cornell
University, Ithaca, New York 14853
*
This work was supported by National Institutes of Health
Grant DK 27083.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. A. Goebl, C. M. Babbey, A. Datta-Mannan, D. R. Witcher, V. J. Wroblewski, and K. W. Dunn Neonatal Fc Receptor Mediates Internalization of Fc in Transfected Human Endothelial Cells Mol. Biol. Cell, December 1, 2008; 19(12): 5490 - 5505. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhang, M. Hong, P. Duan, Z. Pan, J. Ma, and G. You Organic Anion Transporter OAT1 Undergoes Constitutive and Protein Kinase C-regulated Trafficking through a Dynamin- and Clathrin-dependent Pathway J. Biol. Chem., November 21, 2008; 283(47): 32570 - 32579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Blot and T. E. McGraw Molecular Mechanisms Controlling GLUT4 Intracellular Retention Mol. Biol. Cell, August 1, 2008; 19(8): 3477 - 3487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Nilsson, B. Conradt, A.-F. Ruaud, C. C.-H. Chen, J. Hatzold, J.-L. Bessereau, B. D. Grant, and S. Tuck Caenorhabditis elegans num-1 Negatively Regulates Endocytic Recycling Genetics, May 1, 2008; 179(1): 375 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Inoue, T. Kon, R. Ohkura, H. Yamakawa, O. Ohara, J. Yokota, and K. Sutoh BREK/LMTK2 is a myosin VI-binding protein involved in endosomal membrane trafficking. Genes Cells, May 1, 2008; 13(5): 483 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Muller, U. Mettbach, D. Menzel, and J. Samaj Molecular Dissection of Endosomal Compartments in Plants Plant Physiology, October 1, 2007; 145(2): 293 - 304. [Full Text] [PDF]
|
|
|
|
|
|
M. Takahashi, M. Murate, M. Fukuda, S. B. Sato, A. Ohta, and T. Kobayashi Cholesterol Controls Lipid Endocytosis through Rab11 Mol. Biol. Cell, July 1, 2007; 18(7): 2667 - 2677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Saraste and B. Goud Functional Symmetry of Endomembranes Mol. Biol. Cell, April 1, 2007; 18(4): 1430 - 1436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Fisher and S. S. Margulies Modeling the effect of stretch and plasma membrane tension on Na+-K+-ATPase activity in alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L40 - L53. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Bertrand, C. Laboisse, U. Hopfer, R. J. Bridges, and R. A. Frizzell Methods for Detecting Internalized, FM 1-43 Stained Particles in Epithelial Cells and Monolayers Biophys. J., November 15, 2006; 91(10): 3872 - 3883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kim, H. Kim, B. Chang, N. Ahn, S. Hwang, G. Di Paolo, and S. Chang Regulation of transferrin recycling kinetics by PtdIns[4,5]P2 availability FASEB J, November 1, 2006; 20(13): 2399 - 2401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wakabayashi, H. Kipp, and I. M. Arias Transporters on Demand: Intracellular Reservoirs and Cycling of Bile Canalicular ABC Transporters J. Biol. Chem., September 22, 2006; 281(38): 27669 - 27673. [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Palermo, S. L. Hafenstein, and C. R. Parrish Purified feline and canine transferrin receptors reveal complex interactions with the capsids of canine and feline parvoviruses that correspond to their host ranges. J. Virol., September 1, 2006; 80(17): 8482 - 8492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Babbey, N. Ahktar, E. Wang, C. C.-H. Chen, B. D. Grant, and K. W. Dunn Rab10 Regulates Membrane Transport through Early Endosomes of Polarized Madin-Darby Canine Kidney Cells Mol. Biol. Cell, July 1, 2006; 17(7): 3156 - 3175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Coleman, R. Madrid, N. Van Damme, R. S. Mitchell, J. Bouchet, C. Servant, S. Pillai, S. Benichou, and J. C. Guatelli Modulation of Cellular Protein Trafficking by Human Immunodeficiency Virus Type 1 Nef: Role of the Acidic Residue in the ExxxLL Motif J. Virol., February 15, 2006; 80(4): 1837 - 1849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Naslavsky, J. Rahajeng, M. Sharma, M. Jovic, and S. Caplan Interactions between EHD Proteins and Rab11-FIP2: A Role for EHD3 in Early Endosomal Transport Mol. Biol. Cell, January 1, 2006; 17(1): 163 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hao, X. Li, M. A. Rizzo, J. V. Rocheleau, B. M. Dawant, and D. W. Piston Regulation of two insulin granule populations within the reserve pool by distinct calcium sources J. Cell Sci., December 15, 2005; 118(24): 5873 - 5884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Pilzer and Z. Fishelson Mortalin/GRP75 promotes release of membrane vesicles from immune attacked cells and protection from complement-mediated lysis Int. Immunol., September 1, 2005; 17(9): 1239 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Castle and D. Castle Ubiquitously expressed secretory carrier membrane proteins (SCAMPs) 1-4 mark different pathways and exhibit limited constitutive trafficking to and from the cell surface J. Cell Sci., August 15, 2005; 118(16): 3769 - 3780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-Y. Chang, P. C. Reid, S. Sugii, N. Ohgami, J. C. Cruz, and C. C. Y. Chang Niemann-Pick Type C Disease and Intracellular Cholesterol Trafficking J. Biol. Chem., June 3, 2005; 280(22): 20917 - 20920. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Trejo Internal PDZ Ligands: Novel Endocytic Recycling Motifs for G Protein-Coupled Receptors Mol. Pharmacol., May 1, 2005; 67(5): 1388 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wustner Mathematical Analysis of Hepatic High Density Lipoprotein Transport Based on Quantitative Imaging Data J. Biol. Chem., February 25, 2005; 280(8): 6766 - 6779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Madrid, K. Janvier, D. Hitchin, J. Day, S. Coleman, C. Noviello, J. Bouchet, A. Benmerah, J. Guatelli, and S. Benichou Nef-induced Alteration of the Early/Recycling Endosomal Compartment Correlates with Enhancement of HIV-1 Infectivity J. Biol. Chem., February 11, 2005; 280(6): 5032 - 5044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Gage, E. A. Matveeva, S. W. Whiteheart, and M. von Zastrow Type I PDZ Ligands Are Sufficient to Promote Rapid Recycling of G Protein-coupled Receptors Independent of Binding to N-Ethylmaleimide-sensitive Factor J. Biol. Chem., February 4, 2005; 280(5): 3305 - 3313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pagano, P. Crottet, C. Prescianotto-Baschong, and M. Spiess In Vitro Formation of Recycling Vesicles from Endosomes Requires Adaptor Protein-1/Clathrin and Is Regulated by Rab4 and the Connector Rabaptin-5 Mol. Biol. Cell, November 1, 2004; 15(11): 4990 - 5000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Coumailleau, V. Das, A. Alcover, G. Raposo, S. Vandormael-Pournin, S. Le Bras, P. Baldacci, A. Dautry-Varsat, C. Babinet, and M. Cohen-Tannoudji Over-Expression of Rififylin, a New RING Finger and FYVE-like Domain-containing Protein, Inhibits Recycling from the Endocytic Recycling Compartment Mol. Biol. Cell, October 1, 2004; 15(10): 4444 - 4456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Choudhury, D. K. Sharma, D. L. Marks, and R. E. Pagano Elevated Endosomal Cholesterol Levels in Niemann-Pick Cells Inhibit Rab4 and Perturb Membrane Recycling Mol. Biol. Cell, October 1, 2004; 15(10): 4500 - 4511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kalin, J. Fernandes, S. Hrafnsdottir, and G. van Meer Natural Phosphatidylcholine Is Actively Translocated across the Plasma Membrane to the Surface of Mammalian Cells J. Biol. Chem., August 6, 2004; 279(32): 33228 - 33236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Fisher, I. Levitan, and S. S. Margulies Plasma Membrane Surface Increases with Tonic Stretch of Alveolar Epithelial Cells Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2): 200 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hueffer, L. M. Palermo, and C. R. Parrish Parvovirus Infection of Cells by Using Variants of the Feline Transferrin Receptor Altering Clathrin-Mediated Endocytosis, Membrane Domain Localization, and Capsid-Binding Domains J. Virol., June 1, 2004; 78(11): 5601 - 5611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Naslavsky, M. Boehm, P. S. Backlund Jr., and S. Caplan Rabenosyn-5 and EHD1 Interact and Sequentially Regulate Protein Recycling to the Plasma Membrane Mol. Biol. Cell, May 1, 2004; 15(5): 2410 - 2422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Frasch, P. M. Henson, K. Nagaosa, M. B. Fessler, N. Borregaard, and D. L. Bratton Phospholipid Flip-Flop and Phospholipid Scramblase 1 (PLSCR1) Co-localize to Uropod Rafts in Formylated Met-Leu-Phe-stimulated Neutrophils J. Biol. Chem., April 23, 2004; 279(17): 17625 - 17633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hao, S. Mukherjee, Y. Sun, and F. R. Maxfield Effects of Cholesterol Depletion and Increased Lipid Unsaturation on the Properties of Endocytic Membranes J. Biol. Chem., April 2, 2004; 279(14): 14171 - 14178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wustner, M. Mondal, A. Huang, and F. R. Maxfield Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells J. Lipid Res., March 1, 2004; 45(3): 427 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Rosen, V. Glukhman, T. Feldmann, E. Fridman, and D. Lichtstein Cardiac Steroids Induce Changes in Recycling of the Plasma Membrane in Human NT2 Cells Mol. Biol. Cell, March 1, 2004; 15(3): 1044 - 1054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. X. Lin, W. G. Mallet, A. Y. Huang, and F. R. Maxfield Endocytosed Cation-Independent Mannose 6-Phosphate Receptor Traffics via the Endocytic Recycling Compartment en Route to the trans-Golgi Network and a Subpopulation of Late Endosomes Mol. Biol. Cell, February 1, 2004; 15(2): 721 - 733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-L. Xiao, P. Biancani, and J. Behar Role of PGE2 on gallbladder muscle cytoprotection of guinea pigs Am J Physiol Gastrointest Liver Physiol, January 1, 2004; 286(1): G82 - G88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Remacle, G. Murphy, and C. Roghi Membrane type I-matrix metalloproteinase (MT1-MMP) is internalised by two different pathways and is recycled to the cell surface J. Cell Sci., October 1, 2003; 116(19): 3905 - 3916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mohammad-Panah, R. Harrison, S. Dhani, C. Ackerley, L.-J. Huan, Y. Wang, and C. E. Bear The Chloride Channel ClC-4 Contributes to Endosomal Acidification and Trafficking J. Biol. Chem., August 1, 2003; 278(31): 29267 - 29277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Loder and H. E. Melikian The Dopamine Transporter Constitutively Internalizes and Recycles in a Protein Kinase C-regulated Manner in Stably Transfected PC12 Cell Lines J. Biol. Chem., June 6, 2003; 278(24): 22168 - 22174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Rocheleau, P. W. Wiseman, and N. O. Petersen Isolation of Bright Aggregate Fluctuations in a Multipopulation Image Correlation Spectroscopy System Using Intensity Subtraction Biophys. J., June 1, 2003; 84(6): 4011 - 4022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sun, M. Hao, Y. Luo, C.-p. Liang, D. L. Silver, C. Cheng, F. R. Maxfield, and A. R. Tall Stearoyl-CoA Desaturase Inhibits ATP-binding Cassette Transporter A1-mediated Cholesterol Efflux and Modulates Membrane Domain Structure J. Biol. Chem., February 14, 2003; 278(8): 5813 - 5820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. van Dam, T. ten Broeke, K. Jansen, P. Spijkers, and W. Stoorvogel Endocytosed Transferrin Receptors Recycle via Distinct Dynamin and Phosphatidylinositol 3-Kinase-dependent Pathways J. Biol. Chem., December 6, 2002; 277(50): 48876 - 48883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. van der Luit, M. Budde, P. Ruurs, M. Verheij, and W. J. van Blitterswijk Alkyl-lysophospholipid Accumulates in Lipid Rafts and Induces Apoptosis via Raft-dependent Endocytosis and Inhibition of Phosphatidylcholine Synthesis J. Biol. Chem., October 11, 2002; 277(42): 39541 - 39547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Roy, B. Wyse, and J. F. Hancock H-Ras Signaling and K-Ras Signaling Are Differentially Dependent on Endocytosis Mol. Cell. Biol., July 15, 2002; 22(14): 5128 - 5140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sheff, L. Pelletier, C. B. O'Connell, G. Warren, and I. Mellman Transferrin receptor recycling in the absence of perinuclear recycling endosomes J. Cell Biol., March 4, 2002; 156(5): 797 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hao, S. Mukherjee, and F. R. Maxfield Cholesterol depletion induces large scale domain segregation in living cell membranes PNAS, November 6, 2001; 98(23): 13072 - 13077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Schroeder, A. M. Gallegos, B. P. Atshaves, S. M. Storey, A. L. McIntosh, A. D. Petrescu, H. Huang, O. Starodub, H. Chao, H. Yang, et al. Recent Advances in Membrane Microdomains: Rafts, Caveolae, and Intracellular Cholesterol Trafficking Experimental Biology and Medicine, November 1, 2001; 226(10): 873 - 890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Lampson, J. Schmoranzer, A. Zeigerer, S. M. Simon, and T. E. McGraw Insulin-regulated Release from the Endosomal Recycling Compartment Is Regulated by Budding of Specialized Vesicles Mol. Biol. Cell, November 1, 2001; 12(11): 3489 - 3501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zha, J. Genest Jr., and R. McPherson Endocytosis Is Enhanced in Tangier Fibroblasts. POSSIBLE ROLE OF ATP-BINDING CASSETTE PROTEIN A1 IN ENDOSOMAL VESICULAR TRANSPORT J. Biol. Chem., October 12, 2001; 276(42): 39476 - 39483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Citores, D Khnykin, V Sorensen, J Wesche, O Klingenberg, A Wiedlocha, and S Olsnes Modulation of intracellular transport of acidic fibroblast growth factor by mutations in the cytoplasmic receptor domain J. Cell Sci., January 5, 2001; 114(9): 1677 - 1689. [Abstract] [PDF]
|
|
|
|
|
|
B. P. Ceresa, M. Lotscher, and S. L. Schmid Receptor and Membrane Recycling Can Occur with Unaltered Efficiency Despite Dramatic Rab5(Q79L)-induced Changes in Endosome Geometry J. Biol. Chem., March 23, 2001; 276(13): 9649 - 9654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kilic, R. B. Doctor, and J. G. Fitz Insulin Stimulates Membrane Conductance in a Liver Cell Line. EVIDENCE FOR INSERTION OF ION CHANNELS THROUGH A PHOSPHOINOSITIDE 3-KINASE-DEPENDENT MECHANISM J. Biol. Chem., July 13, 2001; 276(29): 26762 - 26768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Gage, K.-A. Kim, T. T. Cao, and M. von Zastrow A Transplantable Sorting Signal That Is Sufficient to Mediate Rapid Recycling of G Protein-coupled Receptors J. Biol. Chem., November 21, 2001; 276(48): 44712 - 44720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hao, S. X. Lin, O. J. Karylowski, D. Wustner, T. E. McGraw, and F. R. Maxfield Vesicular and Non-vesicular Sterol Transport in Living Cells. THE ENDOCYTIC RECYCLING COMPARTMENT IS A MAJOR STEROL STORAGE ORGANELLE J. Biol. Chem., January 4, 2002; 277(1): 609 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sheff, L. Pelletier, C. B. O'Connell, G. Warren, and I. Mellman Transferrin receptor recycling in the absence of perinuclear recycling endosomes J. Cell Biol., March 4, 2002; 156(5): 797 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. X. Lin, G. G. Gundersen, and F. R. Maxfield Export from Pericentriolar Endocytic Recycling Compartment to Cell Surface Depends on Stable, Detyrosinated (Glu) Microtubules and Kinesin Mol. Biol. Cell, January 1, 2002; 13(1): 96 - 109. |
