JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M909687199 on March 16, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/20/15384    most recent
M909687199v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Becker, N. A.
Right arrow Articles by Maher, L. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Becker, N. A.
Right arrow Articles by Maher, L. J., III
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 20, 15384-15391, May 19, 2000

Altered Sensitivity to Single-strand-specific Reagents Associated with the Genomic Vascular Smooth Muscle alpha -Actin Promoter during Myofibroblast Differentiation*

Nicole A. Becker, Robert J. Kelm Jr., Julie A. VranaDagger , Michael J. Getz, and L. James Maher III§

From the Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, Minnesota 55905

Stimulation of quiescent AKR-2B mouse fibroblasts with transforming growth factor beta 1 results in uniform conversion to a myofibroblast-like phenotype as judged by a rapid accumulation of smooth muscle alpha -actin mRNA and protein. Because transcriptional regulation of the smooth muscle alpha -actin gene in these cells might be mediated by single-stranded DNA-binding proteins, we have examined the sensitivity of genomic DNA to chemical reagents with specificity for unpaired bases in a region of the promoter previously implicated in Puralpha , Purbeta , and MSY1 binding in vitro (Kelm, R. J., Jr., Cogan, J. G., Elder, P. K., Strauch, A. R., and Getz, M. J. (1999) J. Biol. Chem. 274, 14238-14245). Our data reveal specific differences between purified DNA treated in vitro and nucleoprotein complexes treated in living cells. Although some differences were observed in quiescent cells, treatment with transforming growth factor beta 1 resulted in the development of additional sensitivity within 1 h. This enhancement was most pronounced in bases immediately upstream of an MCAT enhancer element-containing polypurine-polypyrimidine tract. A TATA-proximal element of similar base distribution showed no such hyperreactivities. These results suggest that activation of the endogenous smooth muscle alpha -actin gene during myofibroblast conversion is accompanied by specific structural changes in the promoter that are consistent with a decline in single-stranded DNA repressor protein binding.

* This work was supported by National Institutes of Health Grants GM48714 and GM54411 (to L. J. M.) and HL54281 (to M. J. G.), a grant from the American Heart Association, Northland Affiliate, Inc. (to R. J. K.), and by the Mayo Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

This paper is dedicated to the memory of Dr. Michael John Getz.

Dagger Present address: Dept. of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, NH 03755.

§ To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, Mayo Foundation, 200 First St. SW, Rochester, MN 55905. Tel.: 507-284-9041; Fax: 507-284-2053; E-mail: maher@mayo.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Zhang, J. J. David, S. V. Subramanian, X. Liu, M. D. Fuerst, X. Zhao, C. V. Leier, C. G. Orosz, R. J. Kelm Jr., and A. R. Strauch
Serum response factor neutralizes Pur{alpha}- and Pur{beta}-mediated repression of the fetal vascular smooth muscle {alpha}-actin gene in stressed adult cardiomyocytes
Am J Physiol Cell Physiol, March 1, 2008; 294(3): C702 - C714.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Knapp, J. E. Ramsey, S.-X. Wang, A. R. Strauch, and R. J. Kelm Jr.
Structure-Function Analysis of Mouse Pur II: CONFORMATION ALTERING MUTATIONS DISRUPT SINGLE-STRANDED DNA AND PROTEIN INTERACTIONS CRUCIAL TO SMOOTH MUSCLE {alpha}-ACTIN GENE REPRESSION
J. Biol. Chem., December 7, 2007; 282(49): 35899 - 35909.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. E. Ramsey, M. A. Daugherty, and R. J. Kelm Jr.
Hydrodynamic Studies on the Quaternary Structure of Recombinant Mouse Purbeta
J. Biol. Chem., January 19, 2007; 282(3): 1552 - 1560.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Knapp, J. E. Ramsey, S.-X. Wang, K. E. Godburn, A. R. Strauch, and R. J. Kelm Jr.
Nucleoprotein Interactions Governing Cell Type-dependent Repression of the Mouse Smooth Muscle {alpha}-Actin Promoter by Single-stranded DNA-binding Proteins Pur{alpha} and Purbeta
J. Biol. Chem., March 24, 2006; 281(12): 7907 - 7918.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Zhang, X. Liu, J. G. Cogan, M. D. Fuerst, J. A. Polikandriotis, R. J. Kelm Jr., and A. R. Strauch
YB-1 Coordinates Vascular Smooth Muscle {alpha}-Actin Gene Activation by Transforming Growth Factor {beta}1 and Thrombin during Differentiation of Human Pulmonary Myofibroblasts
Mol. Biol. Cell, October 1, 2005; 16(10): 4931 - 4940.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-X. Wang, P. K. Elder, Y. Zheng, A. R. Strauch, and R. J. Kelm Jr.
Cell Cycle-mediated Regulation of Smooth Muscle {alpha}-Actin Gene Transcription in Fibroblasts and Vascular Smooth Muscle Cells Involves Multiple Adenovirus E1A-interacting Cofactors
J. Biol. Chem., February 18, 2005; 280(7): 6204 - 6214.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. V. Subramanian, J. A. Polikandriotis, R. J. Kelm Jr., J. J. David, C. G. Orosz, and A. R. Strauch
Induction of Vascular Smooth Muscle {alpha}-Actin Gene Transcription in Transforming Growth Factor {beta}1-Activated Myofibroblasts Mediated by Dynamic Interplay between the Pur Repressor Proteins and Sp1/Smad Coactivators
Mol. Biol. Cell, October 1, 2004; 15(10): 4532 - 4543.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Kelm Jr., S.-X. Wang, J. A. Polikandriotis, and A. R. Strauch
Structure/Function Analysis of Mouse Pur{beta}, a Single-stranded DNA-binding Repressor of Vascular Smooth Muscle {alpha}-Actin Gene Transcription
J. Biol. Chem., October 3, 2003; 278(40): 38749 - 38757.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. G. Cogan, S. V. Subramanian, J. A. Polikandriotis, R. J. Kelm Jr., and A. R. Strauch
Vascular Smooth Muscle alpha -Actin Gene Transcription during Myofibroblast Differentiation Requires Sp1/3 Protein Binding Proximal to the MCAT Enhancer
J. Biol. Chem., September 20, 2002; 277(39): 36433 - 36442.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. V. Subramanian, R. J. Kelm Jr., J. A. Polikandriotis, C. G. Orosz, and A. R. Strauch
Reprogramming of vascular smooth muscle {alpha}-actin gene expression as an early indicator of dysfunctional remodeling following heart transplant
Cardiovasc Res, June 1, 2002; 54(3): 539 - 548.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. E. Carlini, M. J. Getz, A. R. Strauch, and R. J. Kelm Jr.
Cryptic MCAT Enhancer Regulation in Fibroblasts and Smooth Muscle Cells. SUPPRESSION OF TEF-1 MEDIATED ACTIVATION BY THE SINGLE-STRANDED DNA-BINDING PROTEINS, Puralpha , Purbeta , and MSY1
J. Biol. Chem., March 1, 2002; 277(10): 8682 - 8692.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.