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J Biol Chem, Vol. 275, Issue 20, 15578-15585, May 19, 2000

p53 Suppresses the c-Myb-induced Activation of Heat Shock Transcription Factor 3^*

Jun Tanikawa^§, Emi Ichikawa-Iwata, Chie Kanei-Ishii^¶, Akira Nakai, Shu-ichi Matsuzawa^**, John C. Reed^**, and Shunsuke Ishii^¶

From the  Laboratory of Molecular Genetics, RIKEN Tsukuba Life Sciences Center and ^¶ Core Research for Evolutionary Science and Technology Project, Japan Science and Technology, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan, the  Department of Molecular and Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8397, Japan, and the ^** Burnham Institute, La Jolla, California 92037

Expression of heat shock proteins (HSPs) is controlled by heat shock transcription factors (HSFs). Vertebrates express multiple HSFs whose activities may be regulated by distinct signals. HSF3 is specifically activated in unstressed proliferating cells by direct binding to the c-myb proto-oncogene product (c-Myb), which plays an important role in cellular proliferation. This suggests that the c-Myb-induced HSF3 activation may contribute to the growth-regulated expression of HSPs. Here we report that the p53 tumor suppressor protein directly binds to HSF3 and blocks the interaction between c-Myb and HSF3. In addition, p53 stimulates the degradation of c-Myb through a proteasome-dependent mechanism, which is, at least partly, mediated by induction of Siah in certain types of cells. Induction of p53 by a genotoxic reagent in DT40 cells disrupts the HSF3-c-Myb interaction and down-regulates the expression of certain HSPs. Mutated forms of p53 found in certain tumors did not inhibit c-Myb-induced HSF3 activation. The regulation of HSF3 activity by c-Myb and p53 sheds light on the molecular events that govern HSP expression during cellular proliferation and apoptosis.

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^* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^§ Supported by the RIKEN Special Researcher's Basic Science Program.

To whom correspondence should be addressed: Lab. of Molecular Genetics, RIKEN Tsukuba Life Sciences Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan. Tel.: 81-298-36-9031; Fax: 81-298-36-9030; E-mail: sishii@rtc.riken.go.jp.

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Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.

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