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J. Biol. Chem., Vol. 275, Issue 21, 15613-15620, May 26, 2000

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The Promoter for Constitutive Expression of the Human ICln Gene CLNS1A^*

Elke Scandella, Ulrich Olaf Nagl^§, Bernhard Oehl, Fredericke Bergmann, Martin Gschwentner, Johannes Fürst, Andreas Schmarda, Markus Ritter, Siegfried Waldegger^§, Florian Lang^§, Peter Deetjen, and Markus Paulmichl^¶

From the  Department of Physiology, University of Innsbruck, Fritz-Pregl Strasse 3, A-6020 Innsbruck, Austria and the ^§ Department of Physiology I, University of Tübingen, Gmelinstrasse 5, D-72076 Tübingen, Germany

The ICln protein is expressed ubiquitously in mammals. Experiments designed to knock down the ICln protein in NIH 3T3 fibroblasts as well as in epithelial cells led to the conclusion that this protein is crucially involved in volume regulation after cytoplasmic swelling. Reconstitution of the ICln protein in lipid bilayers revealed the ion channel nature of ICln. Here we describe a new human promoter sequence, composed of 89 nucleotides, which is responsible for a highly constitutive expression of the ICln protein. The promoter sequence lacks a TATA box, and the transcription can be effected at multiple sites. In addition to the starting sites, upstream sequence elements are mandatory for an efficient transcription of the ICln gene (CLNS1A). These new nucleotide elements were defined by site-directed mutagenesis.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AF148460 (human) and AF148459 (monkey).

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