JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M001847200 on March 16, 2000

J. Biol. Chem., Vol. 275, Issue 21, 15905-15911, May 26, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/21/15905    most recent
M001847200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, D.
Right arrow Articles by Warren, R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, D.
Right arrow Articles by Warren, R. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Homeostatic Modulation of Cell Surface KDR and Flt1 Expression and Expression of the Vascular Endothelial Cell Growth Factor (VEGF) Receptor mRNAs by VEGF*

Dongfang WangDagger , David B. Donner§, and Robert S. WarrenDagger

From the Dagger  Surgical Oncology Laboratory, Department of Surgery, University of California at San Francisco, San Francisco, California 94143-0790 and the § Department of Microbiology and Immunology and the Walther Oncology Center, Indiana University, School of Medicine, Indianapolis, Indiana 46202

Vascular endothelial cell growth factor (VEGF) is a potent angiogenic factor expressed during embryonic development, during wound healing, and in pathologies dependent on neovascularization, including cancer. Regulation of the receptor tyrosine kinases, KDR and Flt-1, to which VEGF binds on endothelial cells is incompletely understood. Chronic incubation with tumor-conditioned medium or VEGF diminished 125I-VEGF binding to human umbilical vein endothelial cells, incorporation of 125I-VEGF into covalent complexes with KDR and Flt1, and immunoreactive KDR in cell lysates. Receptor down-regulation desensitized VEGF activation of mitogen-activated protein kinase (extracellular signal-regulated kinases 1 and 2) and p38 mitogen-activated protein kinase. Preincubation with VEGF or tumor-conditioned medium down-regulated cell surface receptor expression but up-regulated KDR and Flt-1 mRNAs, an effect abrogated by a neutralizing VEGF antibody. Removal of VEGF from the medium led to recovery of 125I-VEGF binding and resensitization of human umbilical vein endothelial cells. Recovery of receptor expression was inhibited by cycloheximide, indicating that augmented VEGF receptor mRNAs, and not receptor recycling from a cytoplasmic pool, restored responsiveness. As the VEGF receptors promote endothelial cell survival, proliferation, and other events necessary for angiogenesis, the noncoordinate regulation of VEGF receptor proteins and mRNAs suggests that human umbilical vein endothelial cells are protected against inappropriate or prolonged loss of VEGF receptors by a homeostatic mechanism important to endothelial cell function.

* This work was supported by NCI, National Institutes of Health, Grants CA52863 and CA 84019 (to R. S. W.) and CA67891 and CA73023 (to D. B. D.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Surgery, University of California, San Francisco, 533 Parnassus Ave., Rm. U-372, San Francisco, CA 94143-0790. Tel.: 415-476-0800; Fax: 415-476-8696; E-mail: warrenr@surgery.ucsf.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. A. Radek, E. J. Kovacs, R. L. Gallo, and L. A. DiPietro
Acute ethanol exposure disrupts VEGF receptor cell signaling in endothelial cells
Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H174 - H184.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. Rask-Madsen and G. L. King
Differential Regulation of VEGF Signaling by PKC-{alpha} and PKC-{epsilon} in Endothelial Cells
Arterioscler. Thromb. Vasc. Biol., May 1, 2008; 28(5): 919 - 924.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Majka, K. Fox, B. McGuire, J. Crossno Jr., P. McGuire, and A. Izzo
Pleiotropic role of VEGF-A in regulating fetal pulmonary mesenchymal cell turnover
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1183 - L1192.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Mukherjee, M. Tessema, and A. Wandinger-Ness
Vesicular Trafficking of Tyrosine Kinase Receptors and Associated Proteins in the Regulation of Signaling and Vascular Function
Circ. Res., March 31, 2006; 98(6): 743 - 756.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
D. Roth, M. Piekarek, M. Paulsson, H. Christ, T. Krieg, W. Bloch, J. M. Davidson, and S. A. Eming
Plasmin Modulates Vascular Endothelial Growth Factor-A-Mediated Angiogenesis during Wound Repair
Am. J. Pathol., February 1, 2006; 168(2): 670 - 684.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. C. Carpenter, S. Schomberg, and K. R. Stenmark
Endothelin-mediated increases in lung VEGF content promote vascular leak in young rats exposed to viral infection and hypoxia
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1075 - L1082.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Minami, T. Murakami, K. Horiuchi, M. Miura, T. Noguchi, J.-i. Miyazaki, T. Hamakubo, W. C. Aird, and T. Kodama
Interaction between Hex and GATA Transcription Factors in Vascular Endothelial Cells Inhibits flk-1/KDR-mediated Vascular Endothelial Growth Factor Signaling
J. Biol. Chem., May 14, 2004; 279(20): 20626 - 20635.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. A. Castilla, F. Neria, G. Renedo, D. S. Pereira, F. R. Gonzalez-Pacheco, S. Jimenez, P. Tramon, J. J. P. Deudero, M. V. A. Arroyo, S. Yague, et al.
Tumor-induced endothelial cell activation: role of vascular endothelial growth factor
Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1170 - C1176.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
C. Y. Cheung
Vascular Endothelial Growth Factor Activation of Intramembranous Absorption: A Critical Pathway for Amniotic Fluid Volume Regulation
Reproductive Sciences, February 1, 2004; 11(2): 63 - 74.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
M. El-Mousawi, L. Tchistiakova, L. Yurchenko, G. Pietrzynski, M. Moreno, D. Stanimirovic, D. Ahmad, and V. Alakhov
A Vascular Endothelial Growth Factor High Affinity Receptor 1-specific Peptide with Antiangiogenic Activity Identified Using a Phage Display Peptide Library
J. Biol. Chem., November 21, 2003; 278(47): 46681 - 46691.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Lucerna, D. Mechtcheriakova, A. Kadl, G. Schabbauer, R. Schafer, F. Gruber, Y. Koshelnick, H.-D. Muller, K. Issbrucker, M. Clauss, et al.
NAB2, a Corepressor of EGR-1, Inhibits Vascular Endothelial Growth Factor-mediated Gene Induction and Angiogenic Responses of Endothelial Cells
J. Biol. Chem., March 21, 2003; 278(13): 11433 - 11440.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. C. Lockhart, R. D. Braun, D. Yu, J. R. Ross, M. W. Dewhirst, J. S. Humphrey, S. Thompson, K. M. Williams, B. Klitzman, F. Yuan, et al.
Reduction of Wound Angiogenesis in Patients Treated with BMS-275291, a Broad Spectrum Matrix Metalloproteinase Inhibitor
Clin. Cancer Res., February 1, 2003; 9(2): 586 - 593.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Minami, D. J. Donovan, J. C. Tsai, R. D. Rosenberg, and W. C. Aird
Differential regulation of the von Willebrand factor and Flt-1 promoters in the endothelium of hypoxanthine phosphoribosyltransferase-targeted mice
Blood, December 1, 2002; 100(12): 4019 - 4025.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
G.C. Weston, I. Haviv, and P.A.W. Rogers
Microarray analysis of VEGF-responsive genes in myometrial endothelial cells
Mol. Hum. Reprod., September 1, 2002; 8(9): 855 - 863.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
D. Wang, R. E. Lehman, D. B. Donner, M. R. Matli, R. S. Warren, and M. L. Welton
Expression and endocytosis of VEGF and its receptors in human colonic vascular endothelial cells
Am J Physiol Gastrointest Liver Physiol, June 1, 2002; 282(6): G1088 - G1096.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
N. R. Nayak and R. M. Brenner
Vascular Proliferation and Vascular Endothelial Growth Factor Expression in the Rhesus Macaque Endometrium
J. Clin. Endocrinol. Metab., April 1, 2002; 87(4): 1845 - 1855.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. Masood, J. Cai, T. Zheng, D. L. Smith, D. R. Hinton, and P. S. Gill
Vascular endothelial growth factor (VEGF) is an autocrine growth factor for VEGF receptor-positive human tumors
Blood, September 15, 2001; 98(6): 1904 - 1913.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. D. Mayo, K. M. Kessler, R. Pincheira, R. S. Warren, and D. B. Donner
Vascular Endothelial Cell Growth Factor Activates CRE-binding Protein by Signaling through the KDR Receptor Tyrosine Kinase
J. Biol. Chem., June 29, 2001; 276(27): 25184 - 25189.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.