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J. Biol. Chem., Vol. 275, Issue 21, 16044-16049, May 26, 2000
From the Departments of The growing carbonic anhydrase (CA) gene family
includes 11 enzymatically active isozymes in mammals. Each of them has
a characteristic cellular and subcellular distribution pattern. In this
report, we demonstrate for the first time a nuclear protein with CA
activity. A polypeptide recognized by CA II antibodies was purified
from several rat tissues using CA inhibitor affinity chromatography. This polypeptide of apparent 66 kDa mass was characterized using amino
acid sequencing and CA activity measurements. It appeared to be
identical to nonO/p54nrb, a previously cloned and
characterized RNA and DNA binding nuclear factor. Recombinant nonO
generated in baculovirus bound to the CA inhibitor affinity
chromatography matrix and revealed detectable CA activity (25 units/mg). Hansson's histochemical staining of rat lymph nodes
followed by light and electron microscopy showed nuclear CA activity in
lymphocytes, suggesting that the nuclear nonO protein is catalytically
active in vivo. These results demonstrate that a previously
known transcription factor is a novel, nonclassical CA. Through its CA
activity, the nonO may function in the maintenance of pH homeostasis in
the nucleus.
Nuclear NonO/p54nrb Protein Is a Nonclassical
Carbonic Anhydrase*
§,¶,
,**,,,,, and
Anatomy and Cell Biology,
¶ Clinical Chemistry, and ** Neurology, University of Oulu,
Oulu, FIN-90014 Finland, Edward A. Doisy Department of
Biochemistry and Molecular Biology, Saint Louis University School of
Medicine, St. Louis, Missouri 63104, and Department of
Molecular Genetics and Microbiology, Institute of Cell and Molecular
Biology, University of Texas, Austin, Texas 78712
*
This work was supported by a grant from the Sigrid Juselius
Foundation (to S. P.) and by National Institutes of Health Grant DK
40163 (to W. S. S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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