|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 21, 16275-16280, May 26, 2000
From the Institute of Pharmacology and Toxicology, Medical Faculty,
Technical University of Aachen, D-52057 Aachen, Germany
GLUT8 is a novel glucose transporter-like protein
that exhibits significant sequence similarity with the members of the
sugar transport facilitator family (29.4% of amino acids identical
with GLUT1). Human and mouse sequence (86.2% identical amino acids) comprise 12 putative membrane-spanning helices and several conserved motifs (sugar transporter signatures), which have previously been shown
to be essential for transport activity, e.g. GRK in loop 2, PETPR in loop 6, QQLSGVN in helix 7, DRAGRR in loop 8, GWGPIPW in helix
10, and PETKG in the C-terminal tail. An expressed sequence tag (STS
A005N15) corresponding with the 3'-untranslated region of GLUT8 has
previously been mapped to human chromosome 9. COS-7 cells transfected
with GLUT8 cDNA expressed a 42-kDa protein exhibiting specific,
glucose-inhibitable cytochalasin B binding (KD = 56.6 ± 18 nM) and reconstitutable glucose transport
activity (8.1 ± 1.4 nmol/(mg protein × 10 s)
versus 1.1 ± 0.1 in control transfections). In human
tissues, a 2.4-kilobase pair transcript was predominantly found in
testis, but not in testicular carcinoma. Lower amounts of the mRNA
were detected in most other tissues including skeletal muscle, heart,
small intestine, and brain. GLUT8 mRNA was found in testis from
adult, but not from prepubertal rats; its expression in human testis
was suppressed by estrogen treatment. It is concluded that GLUT8 is a
sugar transport facilitator with glucose transport activity and a
hormonally regulated testicular function.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) Y17801 and Y17802.
GLUT8, a Novel Member of the Sugar Transport Facilitator
Family with Glucose Transport Activity*
*
This work was supported in part by Deutsche
Forschungsgemeinschaft Grant Jo 117/8-2 (to H. G. J.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Inst. für
Pharmakologie und Toxikologie, Medizinische Fakultät der RWTH
Aachen, Wendlingweg 2, D-52057 Aachen, Germany. Tel.: 49-241-8089120; Fax: 49-241-8888433; E-mail: joost@rwth-aachen.de.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. T. Kim and K. H. Moley Regulation of Facilitative Glucose Transporters and AKT/MAPK/PRKAA Signaling via Estradiol and Progesterone in the Mouse Uterine Epithelium Biol Reprod, July 1, 2009; 81(1): 188 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schmidt, H.-G. Joost, and A. Schurmann GLUT8, the enigmatic intracellular hexose transporter Am J Physiol Endocrinol Metab, April 1, 2009; 296(4): E614 - E618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Kim and K. H Moley Paternal effect on embryo quality in diabetic mice is related to poor sperm quality and associated with decreased glucose transporter expression Reproduction, September 1, 2008; 136(3): 313 - 322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Sung Tae Kim and K. H. Moley The Expression of GLUT8, GLUT9a, and GLUT9b in the Mouse Testis and Sperm Reproductive Sciences, July 1, 2007; 14(5): 445 - 455. [Abstract] [PDF]
|
|
|
|
|
|
E. J. Miller, J. Li, K. M. Sinusas, G. D. Holman, and L. H. Young Infusion of a biotinylated bis-glucose photolabel: a new method to quantify cell surface GLUT4 in the intact mouse heart Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1922 - E1928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-Q. Zhao and A. F. Keating Expression and Regulation of Glucose Transporters in the Bovine Mammary Gland J Dairy Sci, June 1, 2007; 90(13_suppl): E76 - E86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Membrez, E. Hummler, F. Beermann, J.-A. Haefliger, R. Savioz, T. Pedrazzini, and B. Thorens GLUT8 Is Dispensable for Embryonic Development but Influences Hippocampal Neurogenesis and Heart Function. Mol. Cell. Biol., June 1, 2006; 26(11): 4268 - 4276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Benomar, N. Naour, A. Aubourg, V. Bailleux, A. Gertler, J. Djiane, M. Guerre-Millo, and M. Taouis Insulin and Leptin Induce Glut4 Plasma Membrane Translocation and Glucose Uptake in a Human Neuronal Cell Line by a Phosphatidylinositol 3-Kinase- Dependent Mechanism Endocrinology, May 1, 2006; 147(5): 2550 - 2556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsui, T. Nagoshi, E.-G. Hong, I. Luptak, K. Hartil, L. Li, N. Gorovits, M. J. Charron, J. K. Kim, R. Tian, et al. Effects of chronic Akt activation on glucose uptake in the heart Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E789 - E797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Gomez, A. Romero, J. Terrado, and J. E Mesonero Differential expression of glucose transporter GLUT8 during mouse spermatogenesis Reproduction, January 1, 2006; 131(1): 63 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Widmer, M. Uldry, and B. Thorens GLUT8 Subcellular Localization and Absence of Translocation to the Plasma Membrane in PC12 Cells and Hippocampal Neurons Endocrinology, November 1, 2005; 146(11): 4727 - 4736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schiffer, K. Susztak, M. Ranalletta, A. C. Raff, E. P. Bottinger, and M. J. Charron Localization of the GLUT8 glucose transporter in murine kidney and regulation in vivo in nondiabetic and diabetic conditions Am J Physiol Renal Physiol, July 1, 2005; 289(1): F186 - F193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. H. Ing, A. M. Laughlin, D. D. Varner, T. H. Welsh Jr., D. W. Forrest, T. L. Blanchard, and L. Johnson Gene Expression in the Spermatogenically Inactive "Dark" and Maturing "Light" Testicular Tissues of the Prepubertal Colt J Androl, July 1, 2004; 25(4): 535 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Duran, M. Cano, M. J. Peral, and A. A. Ilundain D-mannose transport and metabolism in isolated enterocytes Glycobiology, June 1, 2004; 14(6): 495 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gorovits, L. Cui, J. V. Busik, M. Ranalletta, S. H. de-Mouzon, and M. J. Charron Regulation of Hepatic GLUT8 Expression in Normal and Diabetic Models Endocrinology, May 1, 2003; 144(5): 1703 - 1711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Mann, D. L. Yudilevich, and L. Sobrevia Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells Physiol Rev, January 1, 2003; 83(1): 183 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. V. Busik, L. K. Olson, M. B. Grant, and D. N. Henry Glucose-Induced Activation of Glucose Uptake in Cells from the Inner and Outer Blood-Retinal Barrier Invest. Ophthalmol. Vis. Sci., July 1, 2002; 43(7): 2356 - 2363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Song, O. Kwon, S. Chen, R. Daruwala, P. Eck, J. B. Park, and M. Levine Flavonoid Inhibition of Sodium-dependent Vitamin C Transporter 1 (SVCT1) and Glucose Transporter Isoform 2 (GLUT2), Intestinal Transporters for Vitamin C and Glucose J. Biol. Chem., May 3, 2002; 277(18): 15252 - 15260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-G. Joost, G. I. Bell, J. D. Best, M. J. Birnbaum, M. J. Charron, Y. T. Chen, H. Doege, D. E. James, H. F. Lodish, K. H. Moley, et al. Nomenclature of the GLUT/SLC2A family of sugar/polyol transport facilitators Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E974 - E976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rogers, M. L. Macheda, S. E. Docherty, M. D. Carty, M. A. Henderson, W. C. Soeller, E. M. Gibbs, D. E. James, and J. D. Best Identification of a novel glucose transporter-like protein---GLUT-12 Am J Physiol Endocrinol Metab, March 1, 2002; 282(3): E733 - E738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ibberson, B. M. Riederer, M. Uldry, B. Guhl, J. Roth, and B. Thorens Immunolocalization of GLUTX1 in the Testis and to Specific Brain Areas and Vasopressin- Containing Neurons Endocrinology, January 1, 2002; 143(1): 276 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Shikhman, D. C. Brinson, J. Valbracht, and M. K. Lotz Cytokine Regulation of Facilitated Glucose Transport in Human Articular Chondrocytes J. Immunol., December 15, 2001; 167(12): 7001 - 7008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Turkay Korgun, R. Demir, A. Hammer, G. Dohr, G. Desoye, G. Skofitsch, and T. Hahn Glucose Transporter Expression in Rat Embryo and Uterus During Decidualization, Implantation, and Early Postimplantation Biol Reprod, November 1, 2001; 65(5): 1364 - 1370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. P. Reagan, N. Gorovits, E. K. Hoskin, S. E. Alves, E. B. Katz, C. A. Grillo, G. G. Piroli, B. S. McEwen, and M. J. Charron Localization and regulation of GLUTx1 glucose transporter in the hippocampus of streptozotocin diabetic rats PNAS, February 8, 2001; (2001) 51629798. [Abstract] [Full Text]
|
|
|
|
|
|
I. A. Simpson, S. J. Vannucci, M. R. DeJoseph, and R. A. Hawkins Glucose Transporter Asymmetries in the Bovine Blood-Brain Barrier J. Biol. Chem., April 13, 2001; 276(16): 12725 - 12729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Reagan, N. Gorovits, E. K. Hoskin, S. E. Alves, E. B. Katz, C. A. Grillo, G. G. Piroli, B. S. McEwen, and M. J. Charron Localization and regulation of GLUTx1 glucose transporter in the hippocampus of streptozotocin diabetic rats PNAS, February 27, 2001; 98(5): 2820 - 2825. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |