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J. Biol. Chem., Vol. 275, Issue 21, 16289-16295, May 26, 2000
From the Department of Life Sciences, Ben-Gurion University of the
Negev, Beer-Sheva 84105, Israel
Previous work showed a transient but dramatic
arrest in the synthesis of Rubisco large subunit (LSU) upon transfer of
Chlamydomonas reinhardtii cells from low light (LL) to high
light (HL). Using dichlorofluorescin, a short-term increase in reactive
oxygen species (ROS) was demonstrated, suggesting that their excessive
formation could signal LSU down-regulation. A decrease in LSU synthesis occurred at LL in the presence of methyl viologen and was prevented at
HL by ascorbate. Interfering with D1 function by mutations or by
incubation with DCMU prevented the increase in ROS formation at HL and
the concomitant down-regulation of LSU synthesis. If the electron
transport was blocked further downstream, by mutation in the cytochrome
b6/f or by incubation with
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, ROS
formation increased, and LSU synthesis ceased. The elevation of ROS
occurred concurrently with a change in the redox state of the
glutathione pool, which shifted toward its oxidized form immediately
after the transfer to HL and returned to its original value after
6 h. The decrease in the reduced/oxidized glutathione ratio at HL
was prevented by ascorbate and could be induced at LL by methyl
viologen. We suggest that excess ROS mediate a decrease in the
reduced/oxidized glutathione ratio that in turn signals the
translational arrest of the rbcL transcript.
Glutathione Redox Potential Modulated by Reactive Oxygen Species
Regulates Translation of Rubisco Large Subunit in the Chloroplast*
*
This work was supported by the Doris and Bertie Black Center
for Bioenergetics in Life Sciences at the Ben-Gurion University.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. Life Sciences,
Ben-Gurion University of the Negev, Beer-Sheva, 84105 Israel. Tel.:
972-7-6472663; Fax: 972-7-6472890; E-mail:
shapiram@bgumail.bgu.ac.il.
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