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J. Biol. Chem., Vol. 275, Issue 21, 16296-16301, May 26, 2000
From the Department of Biosciences at Novum, Karolinska Institute,
S-141 57 Huddinge, Sweden
Peroxiredoxins are ubiquitously expressed
proteins that reduce hydroperoxides using disulfur-reducing compounds
as electron donors. Peroxiredoxins (Prxs) have been classified in two
groups dependent on the presence of either one (1-Cys Prx) or two
(2-Cys Prx) conserved cysteine residues. Moreover, 2-Cys Prxs, also
named thioredoxin peroxidases, have peroxide reductase activity with the use of thioredoxin as biological electron donor. However, the
biological reducing agent for the 1-Cys Prx has not yet been identified. We report here the characterization of a 1-Cys Prx from
yeast Saccharomyces cerevisiae that we have named Prx1p. Prx1p is located in mitochondria, and it is overexpressed when cells
use the respiratory pathway, as well as in response to oxidative stress
conditions. We show also that Prx1p has peroxide reductase activity
in vitro using the yeast mitochondrial thioredoxin system as electron donor. In addition, a mutated form of Prx1p containing the
absolutely conserved cysteine as the only cysteine residue also shows
thioredoxin-dependent peroxide reductase activity. This is
the first example of 1-Cys Prx that has thioredoxin peroxidase activity. Finally, exposure of null Prx1p mutant cells to oxidant conditions reveals an important role of the mitochondrial 1-Cys Prx in
protection against oxidative stress.
This paper is dedicated to the memory of Dr. Antonio López Ruiz.
Mitochondria of Saccharomyces cerevisiae Contain
One-conserved Cysteine Type Peroxiredoxin with Thioredoxin Peroxidase
Activity*
*
This work was supported by Swedish Medical Research Council
Project Grant 13X-10370, by funds from the Karolinska Institutet, the
Svenska Institutet, and Åke Wibergs Stiftelse, and by Training and
Mobility of Researchers Marie Curie Research Training Grant ERBFMBICT972824.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Biosciences,
Center for Biotechnology, Karolinska Institutet, Novum, S-141 57 Huddinge, Sweden. Tel.: 46-8-6089162; Fax: 46-8-7745538; E-mail:
giannis.spyrou@cbt.ki.se.
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