JBC Oz Biosciences

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M001266200 on March 13, 2000

J. Biol. Chem., Vol. 275, Issue 21, 16337-16344, May 26, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/21/16337    most recent
M001266200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mullen, R. T.
Right arrow Articles by Trelease, R. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mullen, R. T.
Right arrow Articles by Trelease, R. N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The Sorting Signals for Peroxisomal Membrane-bound Ascorbate Peroxidase Are within Its C-terminal Tail*

Robert T. MullenDagger and Richard N. Trelease§

From the Department of Plant Biology, Arizona State University, Tempe, Arizona 85287-1601

Peroxisomal ascorbate peroxidase (APX) is a carboxyl tail-anchored, type II (Ncytosol-Cmatrix) integral membrane protein that functions in the regeneration of NAD+ in glyoxysomes of germinated oilseeds and protection of peroxisomes in other organisms from toxic H2O2. Recently we showed that cottonseed peroxisomal APX was sorted post-translationally from the cytosol to peroxisomes via a novel reticular/circular membranous network that was interpreted to be a subdomain of the endoplasmic reticulum (ER), named peroxisomal ER (pER). Here we report on the molecular signals responsible for sorting peroxisomal APX. Deletions or site-specific substitutions of certain amino acid residues within the hydrophilic C-terminal-most eight-amino acid residues (includes a positively charged domain found in most peroxisomal integral membrane-destined proteins) abolished sorting of peroxisomal APX to peroxisomes via pER. However, the C-terminal tail was not sufficient for sorting chloramphenicol acetyltransferase to peroxisomes via pER, whereas the peptide plus most of the immediately adjacent 21-amino acid transmembrane domain (TMD) of peroxisomal APX was sufficient for sorting. Replacement of the peroxisomal APX TMD with an artificial TMD (devoid of putative sorting sequences) plus the peroxisomal APX C-terminal tail also sorted chloramphenicol acetyltransferase to peroxisomes via pER, indicating that the peroxisomal APX TMD does not possess essential sorting information. Instead, the TMD appears to confer the proper context required for the conserved positively charged domain to function within peroxisomal APX as an overlapping pER sorting signal and a membrane peroxisome targeting signal type 2.

* This work was supported by National Science Foundation Grant MCB-9728935 and in part by the William N. and Myriam Pennington Foundation (to R. N. T.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Present address: Dept. of Biology, York University, Toronto, Ontario M3J 1P3, Canada.

§ To whom correspondence should be addressed. Tel.: 480-965-2669; Fax: 480-965-6899; E-mail: trelease.dick@asu.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. K. Delille and M. Schrader
Targeting of hFis1 to Peroxisomes Is Mediated by Pex19p
J. Biol. Chem., November 7, 2008; 283(45): 31107 - 31115.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Kashiwayama, K. Asahina, M. Morita, and T. Imanaka
Hydrophobic Regions Adjacent to Transmembrane Domains 1 and 5 Are Important for the Targeting of the 70-kDa Peroxisomal Membrane Protein
J. Biol. Chem., November 16, 2007; 282(46): 33831 - 33844.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. K. Karnik and R. N. Trelease
Arabidopsis peroxin 16 trafficks through the ER and an intermediate compartment to pre-existing peroxisomes via overlapping molecular targeting signals
J. Exp. Bot., May 1, 2007; 58(7): 1677 - 1693.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Maggio, A. Barbante, F. Ferro, L. Frigerio, and E. Pedrazzini
Intracellular sorting of the tail-anchored protein cytochrome b5 in plants: a comparative study using different isoforms from rabbit and Arabidopsis
J. Exp. Bot., April 1, 2007; 58(6): 1365 - 1379.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Narendra, S. Venkataramani, G. Shen, J. Wang, V. Pasapula, Y. Lin, D. Kornyeyev, A. S. Holaday, and H. Zhang
The Arabidopsis ascorbate peroxidase 3 is a peroxisomal membrane-bound antioxidant enzyme and is dispensable for Arabidopsis growth and development
J. Exp. Bot., September 1, 2006; 57(12): 3033 - 3042.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
V. I. Titorenko and R. T. Mullen
Peroxisome biogenesis: the peroxisomal endomembrane system and the role of the ER
J. Cell Biol., July 3, 2006; 174(1): 11 - 17.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Halbach, C. Landgraf, S. Lorenzen, K. Rosenkranz, R. Volkmer-Engert, R. Erdmann, and H. Rottensteiner
Targeting of the tail-anchored peroxisomal membrane proteins PEX26 and PEX15 occurs through C-terminal PEX19-binding sites
J. Cell Sci., June 15, 2006; 119(12): 2508 - 2517.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. J. Vizeacoumar, W. N. Vreden, J. D. Aitchison, and R. A. Rachubinski
Pex19p Binds Pex30p and Pex32p at Regions Required for Their Peroxisomal Localization but Separate from Their Peroxisomal Targeting Signals
J. Biol. Chem., May 26, 2006; 281(21): 14805 - 14812.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. D'ARCY-LAMETA, R. FERRARI-ILIOU, D. CONTOUR-ANSEL, A.-T. PHAM-THI, and Y. ZUILY-FODIL
Isolation and Characterization of Four Ascorbate Peroxidase cDNAs Responsive to Water Deficit in Cowpea Leaves
Ann. Bot., January 1, 2006; 97(1): 133 - 140.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. W. McCartney, J. S. Greenwood, M. R. Fabian, K. A. White, and R. T. Mullen
Localization of the Tomato Bushy Stunt Virus Replication Protein p33 Reveals a Peroxisome-to-Endoplasmic Reticulum Sorting Pathway
PLANT CELL, December 1, 2005; 17(12): 3513 - 3531.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. T. Hwang, S. M. Pelitire, M. P.A. Henderson, D. W. Andrews, J. M. Dyer, and R. T. Mullen
Novel Targeting Signals Mediate the Sorting of Different Isoforms of the Tail-Anchored Membrane Protein Cytochrome b5 to Either Endoplasmic Reticulum or Mitochondria
PLANT CELL, November 1, 2004; 16(11): 3002 - 3019.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
X. Wang, M. A. McMahon, S. N. Shelton, M. Nampaisansuk, J. L. Ballard, and J. M. Goodman
Multiple Targeting Modules on Peroxisomal Proteins Are Not Redundant: Discrete Functions of Targeting Signals within Pmp47 and Pex8p
Mol. Biol. Cell, April 1, 2004; 15(4): 1702 - 1710.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Fukao, M. Hayashi, I. Hara-Nishimura, and M. Nishimura
Novel Glyoxysomal Protein Kinase, GPK1, Identified by Proteomic Analysis of Glyoxysomes in Etiolated Cotyledons of Arabidopsis thaliana
Plant Cell Physiol., October 15, 2003; 44(10): 1002 - 1012.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Murphy, B. A. Phillipson, A. Baker, and R. T. Mullen
Characterization of the Targeting Signal of the Arabidopsis 22-kD Integral Peroxisomal Membrane Protein
Plant Physiology, October 1, 2003; 133(2): 813 - 828.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
N. Borgese, S. Colombo, and E. Pedrazzini
The tale of tail-anchored proteins: coming from the cytosol and looking for a membrane
J. Cell Biol., June 23, 2003; 161(6): 1013 - 1019.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. S. Lisenbee, M. Heinze, and R. N. Trelease
Peroxisomal Ascorbate Peroxidase Resides within a Subdomain of Rough Endoplasmic Reticulum in Wild-Type Arabidopsis Cells
Plant Physiology, June 1, 2003; 132(2): 870 - 882.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Fukao, M. Hayashi, and M. Nishimura
Proteomic Analysis of Leaf Peroxisomal Proteins in Greening Cotyledons of Arabidopsis thaliana
Plant Cell Physiol., July 15, 2002; 43(7): 689 - 696.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Shigeoka, T. Ishikawa, M. Tamoi, Y. Miyagawa, T. Takeda, Y. Yabuta, and K. Yoshimura
Regulation and function of ascorbate peroxidase isoenzymes
J. Exp. Bot., May 15, 2002; 53(372): 1305 - 1319.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Mathur, N. Mathur, and M. Hulskamp
Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants
Plant Physiology, March 1, 2002; 128(3): 1031 - 1045.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. L. Johnson and L. J. Olsen
Building New Models for Peroxisome Biogenesis
Plant Physiology, November 1, 2001; 127(3): 731 - 739.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Wang, M. J. Unruh, and J. M. Goodman
Discrete Targeting Signals Direct Pmp47 to Oleate-induced Peroxisomes in Saccharomyces cerevisiae
J. Biol. Chem., March 30, 2001; 276(14): 10897 - 10905.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.