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J. Biol. Chem., Vol. 275, Issue 22, 16414-16419, June 2, 2000
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From Ribosomal RNAs undergo several nucleotide
modifications including methylation. We identify FtsJ, the first
encoded protein of the ftsJ-hflB heat shock operon, as an
Escherichia coli methyltransferase of the 23 S
rRNA. The methylation reaction requires
S-adenosylmethionine as donor of methyl groups, purified
FtsJ or a S150 supernatant from an FtsJ-producing strain,
and ribosomes from an FtsJ-deficient strain. In vitro, FtsJ
does not efficiently methylate ribosomes purified from a strain
producing FtsJ, suggesting that these ribosomes are already methylated
in vivo by FtsJ. FtsJ is active on ribosomes and on the 50 S ribosomal subunit, but is inactive on free rRNA, suggesting that its
natural substrate is ribosomes or a pre-ribosomal ribonucleoprotein
particle. We identified the methylated nucleotide as
2'-O-methyluridine 2552, by reverse phase high performance liquid chromatography analysis, boronate affinity chromatography, and
hybridization-protection experiments. In view of its newly established
function, FtsJ is renamed RrmJ and its encoding gene, rrmJ.
The FtsJ/RrmJ Heat Shock Protein of Escherichia
coli Is a 23 S Ribosomal RNA Methyltransferase*
§,
,, and**
Biochimie Génétique, Institut
Jacques Monod, Université Paris 7, 2, place Jussieu, 75005 Paris,
¶ Laboratoire des Réseaux de Régulations, Institut de
Génétique et Microbiologie, Université Paris-Sud,
CNRS, UMR8621, 91405 Orsay Cedex, and Laboratoire de Biochimie
Médicale, Faculté de Médecine et Centre Hospitalier
de Bourgogne, 10 boulevard de Lattre de Tassigny,
21034 Dijon, France
*
This work was supported in part by the Programme de
Recherche Fondamentale en Microbiologie, Maladies Infectieuses et
Parasitaires, funded by the Ministère de L'Education Nationale,
de la Recherche et de la Technologie, and by Association pour la
Recherche sur le Cancer Grant 9956.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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