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J. Biol. Chem., Vol. 275, Issue 22, 16459-16465, June 2, 2000
From the Department of Biological Chemistry, The Weizmann Institute
of Science, Rehovot 76100, Israel
TAFII105 is a TFIID-associated
factor highly expressed in B lymphocytes. This subunit is found in a
small portion of TFIID complexes and is homologous to human
TAFII130 and Drosophila TAFII110. In the present study we show that TAFII105 is involved in
transcription activation directed by the B cell-specific octamer
element found in many B cell-specific genes. B cells overexpressing
TAFII105 display higher octamer-dependent
transcription, whereas expression of a C-terminal truncated form of
TAFII105 inhibits octamer transcription in a dominant
negative manner. In addition, antibodies directed against
TAFII105 specifically inhibit octamer-dependent
transcription. Reporter gene analysis revealed that
TAFII105 elevates octamer transcription in the presence of
OCA-B, a cofactor subunit of Oct1 and Oct2 proteins. In
vitro binding assays and functional studies established that the
effect of TAFII105 on octamer activity involves interaction
of TAFII105 with octamer-binding complexes via the
C-terminal activation domain of OCA-B. These findings link
TAFII105 coactivator function to B cell-specific transcription.
Specific Interaction of TAFII105 with OCA-B Is
Involved in Activation of Octamer-dependent
Transcription*
*
This work was supported by grants from the Minerva
Foundation, Germany, by The Israel Cancer Research Fund, and by the Leo and Julia Forcheimer Center for Molecular Genetics.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Incumbent of the Martha S. Sagon Career Development Chair. To whom
correspondence should be addressed. Tel.: 972-8-934-2117; Fax:
972-8-934-4118; E-mail: bcrivka@wiccmail.weizmann.ac.il.
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