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Originally published In Press as doi:10.1074/jbc.M000314200 on March 28, 2000

J. Biol. Chem., Vol. 275, Issue 22, 16658-16665, June 2, 2000
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Characterization of Cdk2-Cyclin E Complexes in Plasma Membrane and Endosomes of Liver Parenchyma
INSULIN-DEPENDENT REGULATION*

Jean-François GaulinDagger , Annie Fiset§, Suzanne Fortier, and Robert L. Faure

From the Unité de Recherche en Pédiatrie, Laboratoire de Biologie Cellulaire, Centre de Recherche du CHUL, Université Laval, Québec G1V 4G2, Canada

Rat liver parenchyma Golgi/endosomes fractions harbor a tyrosine-phosphorylated 34-kDa protein. Screening of Golgi, endosomes (ENs), plasmalemma (PM), and cytosolic (Cyt) fractions revealed the presence of the mitotic kinase Cdk2 in ENs, PM, and Cyt. The fluid phase endocytic marker horseradish peroxidase gained access to the endosomal Cdk2, confirming its localization. Cdk2 was shown to be associated to cyclin E and was active in ENs and PM fractions. The administration of a single dose of insulin (1.5 µg/100 g, body weight) induced a time-dependent activation of the insulin receptor kinase in these structures. Insulin receptor-kinase activation was followed by the inhibition of immunoprecipitated Cdk2-cyclin E kinase activity in PM and the progressive disappearance of cyclin E. In marked contrast, no such effect was observed in ENs. The injection of a phosphotyrosyl phosphatase inhibitor (bpV(phen)) increased the levels of cyclin E in ENs and PM. A massive recruitment of p27kip1 was observed in the Cdk2-cyclin E complexes isolated from PM and Cyt but not from ENs. In vitro, Cdk2-cyclin E complexes have the capacity to inhibit the formation of hybrid structures containing horseradish peroxidase and radioiodinated epidermal growth factor. Therefore, in the PM and ENs of adult rat liver, an active and regulated pool of the mitotic kinase Cdk2-cyclin E and some yet to be defined effectors are present. Cdk2 may contribute to the modulation of transport events and/or maintenance of the topology of endocytic elements.

* This work was supported by National Science and Engineering Research Council of Canada Grant RF: OGPO157551 and a grant from the Fondation pour la Recherche sur les Maladies Infantiles (FRMI).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported by a Formation des Chercheurs et Aide à la Recherche studentship.

§ Supported by a FRMI studentship.

Supported by a scholarship (Junior II) from the Fonds de la Recherche en Santé du Québec. To whom correspondence should be addressed: Centre de Recherche du Centre Hospitalier de l'Université Laval, Unité de Recherche en Pédiatrie, lab. 9800, 2705 boul. Laurier, Ste-Foy, Québec G1V 4G2, Canada. Tel.: 418-654-2152; Fax: 418-654-2753; E-mail: Robert.Faure@crchul.ulaval.ca.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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