Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M000728200 on March 21, 2000

J. Biol. Chem., Vol. 275, Issue 22, 16723-16729, June 2, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/22/16723    most recent
M000728200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Steffensen, R.
Right arrow Articles by Clausen, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steffensen, R.
Right arrow Articles by Clausen, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Cloning and Expression of the Histo-blood Group Pk UDP-galactose:Galbeta 1-4Glcbeta 1-Cer alpha 1,4-Galactosyltransferase
MOLECULAR GENETIC BASIS OF THE p PHENOTYPE*

Rudi SteffensenDagger §, Karine Carlier, Joelle Wiels, Steven B. Levery||, Mark Stroud**, Bertil CedergrenDagger Dagger , Birgitta Nilsson SojkaDagger Dagger , Eric P. BennettDagger , Casper Jersild§, and Henrik ClausenDagger §§

From the Dagger  School of Dentistry, University of Copenhagen, Nørre Allé 20, 2200 Copenhagen N, Denmark, the § Regional Center for Blood Transfusion and Clinical Immunology, Aalborg Hospital, 9000 Aalborg, Denmark,  CNRS UMR 1598, Institut Gustave Roussy, 94805 Villejuif Cedex, France, the || Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, the ** Department of Cell Surface Biochemistry, Northwest Hospital, Seattle, Washington 98125, and the Dagger Dagger  Department of Transfusion Medicine, Umeå University Hospital, S-901 85 Umeå, Sweden

The molecular genetic basis of the P histo-blood group system has eluded characterization despite extensive studies of the biosynthesis of the P1, P, and Pk glycolipids. The main controversy has been whether a single or two distinct UDP-Gal:Galbeta 1-R 4-alpha -galactosyltransferases catalyze the syntheses of the structurally related P1 and Pk antigens. The P1 polymorphism is linked to 22q11.3-ter. Data base searches with the coding region of an alpha 4GlcNAc-transferase identified a novel homologous gene at 22q13.2 designated alpha 4Gal-T1. Expression of full coding constructs of alpha 4Gal-T1 in insect cells revealed it encoded Pk but not P1 synthase activity. Northern analysis showed expression of the transcript correlating with Pk synthase activity and antigen expression in human B cell lines. Transfection of Pk-negative Namalwa cells with alpha 4Gal-T1 resulted in strong Pk expression. A single homozygous missense mutation, M183K, was found in six Swedish individuals of the rare p phenotype, confirming that alpha 4Gal-T1 represented the Pk gene. Sequence analysis of the coding region of alpha 4Gal-T1 in P1+/- individuals did not reveal polymorphisms correlating with P1P2 typing.

* This work was supported by the PhD Foundation Aalborg Sygehus, Aalborg Frivillige Bloddonorers Foundation, Nordjyllands Amts Foundation, Det Obelse Familiefond, Peder Kristen Tøfting, and Dagmar Tøftings Foundation, Ebba and Aksel Schølins Foundation, the Danish Cancer Society, the Velux Foundation, the Danish Research Council, Grant 5 P41 RR05351 from the National Institutes of Health Resource Center for Biomedical Complex Carbohydrates, and Grant ARC9588 from the Association pour la Recherche sur le Cancer.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ245581.

§§ To whom correspondence should be addressed. Tel.: 45-35326835; Fax: 45-35326505; E-mail: henrik.clausen@odont.ku.dk.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
N. Lund, M. L. Olsson, S. Ramkumar, D. Sakac, V. Yahalom, C. Levene, A. Hellberg, X.-Z. Ma, B. Binnington, D. Jung, et al.
The human Pk histo-blood group antigen provides protection against HIV-1 infection
Blood, May 14, 2009; 113(20): 4980 - 4991.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. Johswich, B. Kraft, M. Wuhrer, M. Berger, A. M. Deelder, C. H. Hokke, R. Gerardy-Schahn, and H. Bakker
Golgi targeting of Drosophila melanogaster {beta}4GalNAcTB requires a DHHC protein family-related protein as a pilot
J. Cell Biol., January 12, 2009; 184(1): 173 - 183.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
T. Okuda and K.-i. Nakayama
Identification and characterization of the human Gb3/CD77 synthase gene promoter
Glycobiology, December 1, 2008; 18(12): 1028 - 1035.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
A.-L. Turcot-Dubois, B. Le Moullac-Vaidye, S. Despiau, F. Roubinet, N. Bovin, J. Le Pendu, and A. Blancher
Long-term evolution of the CAZY glycosyltransferase 6 (ABO) gene family from fishes to mammals--a birth-and-death evolution model
Glycobiology, May 1, 2007; 17(5): 516 - 528.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
O. Kovbasnjuk, R. Mourtazina, B. Baibakov, T. Wang, C. Elowsky, M. A. Choti, A. Kane, and M. Donowitz
The glycosphingolipid globotriaosylceramide in the metastatic transformation of colon cancer
PNAS, December 27, 2005; 102(52): 19087 - 19092.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
Y. Fujii, S.-i. Numata, Y. Nakamura, T. Honda, K. Furukawa, T. Urano, J. Wiels, M. Uchikawa, N. Ozaki, S.-i. Matsuo, et al.
Murine glycosyltransferases responsible for the expression of globo-series glycolipids: cDNA structures, mRNA expression, and distribution of their products
Glycobiology, December 1, 2005; 15(12): 1257 - 1267.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. H. Wandall, S. Pizette, J. W. Pedersen, H. Eichert, S. B. Levery, U. Mandel, S. M. Cohen, and H. Clausen
Egghead and Brainiac Are Essential for Glycosphingolipid Biosynthesis in Vivo
J. Biol. Chem., February 11, 2005; 280(6): 4858 - 4863.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Tetaud, T. Falguieres, K. Carlier, Y. Lecluse, J. Garibal, D. Coulaud, P. Busson, R. Steffensen, H. Clausen, L. Johannes, et al.
Two Distinct Gb3/CD77 Signaling Pathways Leading to Apoptosis Are Triggered by Anti-Gb3/CD77 mAb and Verotoxin-1
J. Biol. Chem., November 14, 2003; 278(46): 45200 - 45208.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Iwamura, K. Furukawa, M. Uchikawa, B. N. Sojka, Y. Kojima, J. Wiels, H. Shiku, T. Urano, and K. Furukawa
The Blood Group P1 Synthase Gene Is Identical to the Gb3/CD77 Synthase Gene: A CLUE TO THE SOLUTION OF THE P1/P2/p PUZZLE
J. Biol. Chem., November 7, 2003; 278(45): 44429 - 44438.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. L. W. Cooling, D. S. Zhang, S. J. Naides, and T. A. W. Koerner
Glycosphingolipid expression in acute nonlymphocytic leukemia: common expression of shiga toxin and parvovirus B19 receptors on early myeloblasts
Blood, January 15, 2003; 101(2): 711 - 721.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. H. Wandall, J. W. Pedersen, C. Park, S. B. Levery, S. Pizette, S. M. Cohen, T. Schwientek, and H. Clausen
Drosophila egghead Encodes a beta 1,4-Mannosyltransferase Predicted to Form the Immediate Precursor Glycosphingolipid Substrate for brainiac
J. Biol. Chem., January 10, 2003; 278(3): 1411 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Schwientek, B. Keck, S. B. Levery, M. A. Jensen, J. W. Pedersen, H. H. Wandall, M. Stroud, S. M. Cohen, M. Amado, and H. Clausen
The Drosophila Gene brainiac Encodes a Glycosyltransferase Putatively Involved in Glycosphingolipid Synthesis
J. Biol. Chem., August 30, 2002; 277(36): 32421 - 32429.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Hellberg, J. Poole, and M. L. Olsson
Molecular Basis of the Globoside-deficient Pk Blood Group Phenotype. IDENTIFICATION OF FOUR INACTIVATING MUTATIONS IN THE UDP-N-ACETYLGALACTOSAMINE: GLOBOTRIAOSYLCERAMIDE 3-beta -N-ACETYLGALACTOSAMINYLTRANSFERASE GENE
J. Biol. Chem., August 9, 2002; 277(33): 29455 - 29459.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L.-C. Yu, Y.-C. Twu, C.-Y. Chang, and M. Lin
Molecular basis of the adult i phenotype and the gene responsible for the expression of the human blood group I antigen
Blood, December 15, 2001; 98(13): 3840 - 3845.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. X. Zhang, J. Nakayama, E. Hidaka, S. Kubota, J. Yan, H. Ota, and M. Fukuda
Immunohistochemical Demonstration of {{alpha}}1,4-N-acetylglucosaminyltransferase that Forms GlcNAc{{alpha}}1,4Gal{beta} Residues in Human Gastrointestinal Mucosa
J. Histochem. Cytochem., May 1, 2001; 49(5): 587 - 596.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
K. Furukawa, K. Iwamura, M. Uchikawa, B. N. Sojka, J. Wiels, T. Okajima, T. Urano, and K. Furukawa
Molecular Basis for the p Phenotype. IDENTIFICATION OF DISTINCT AND MULTIPLE MUTATIONS IN THE alpha 1,4-GALACTOSYLTRANSFERASE GENE IN SWEDISH AND JAPANESE INDIVIDUALS
J. Biol. Chem., November 22, 2000; 275(48): 37752 - 37756.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. J. Keusch, S. M. Manzella, K. A. Nyame, R. D. Cummings, and J. U. Baenziger
Cloning of Gb3 Synthase, the Key Enzyme in Globo-series Glycosphingolipid Synthesis, Predicts a Family of alpha 1,4-Glycosyltransferases Conserved in Plants, Insects, and Mammals
J. Biol. Chem., August 11, 2000; 275(33): 25315 - 25321.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Okajima, Y. Nakamura, M. Uchikawa, D. B. Haslam, S.-i. Numata, K. Furukawa, T. Urano, and K. Furukawa
Expression Cloning of Human Globoside Synthase cDNAs. IDENTIFICATION OF beta 3Gal-T3 AS UDP-N-ACETYLGALACTOSAMINE:GLOBOTRIAOSYLCERAMIDE beta 1,3-N-ACETYLGALACTOSAMINYLTRANSFERASE
J. Biol. Chem., December 15, 2000; 275(51): 40498 - 40503.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement