|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 22, 16788-16794, June 2, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Recent evidence has indicated a requirement for a
Src family kinase in initiating Ca2+ release at
fertilization in starfish eggs (Giusti, A. F., Carroll, D. J., Abassi, Y. A., Terasaki, M., Foltz, K. R., and Jaffe,
L. A. (1999) J. Biol. Chem. 274, 29318-29322).
We now show that injection of Src protein into starfish eggs initiates
Ca2+ release and DNA synthesis, as occur at fertilization.
These responses depend on the phosphorylation state of the Src protein;
only the kinase active form is effective. Like Ca2+ release
at fertilization, the Ca2+ release in response to Src
protein injection is inhibited by prior injection of the SH2 domains of
phospholipase C
Evidence That Fertilization Activates Starfish Eggs by Sequential
Activation of a Src-like Kinase and Phospholipase C
*
§,§,§, and§¶
Marine Biological Laboratory, Woods Hole,
Massachusetts 02543, the § Department of Physiology,
University of Connecticut Health Center, Farmington, Connecticut 06032, and the ¶ Department of Biological Structure, Biomolecular
Structure Center, University of Washington,
Seattle, Washington 98195
. These findings support the conclusion that in
starfish, sperm-egg interaction causes egg activation by sequential
activation of a Src-like kinase and phospholipase C. Injection of
the SH2 domain of Src, which inhibits Ca2+ release at
fertilization, does not inhibit Ca2+ release caused by Src
protein injection. This indicates that the requirement for a Src SH2
domain interaction is upstream of Src activation in the pathway leading
to Ca2+ release at fertilization.
*
This work was supported by grants from the National
Institutes of Health (to A. G. and L. A. J.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Whitaker Calcium signalling in early embryos Phil Trans R Soc B, April 12, 2008; 363(1495): 1401 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Meng, J. Luo, C. Li, and W. H Kinsey Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development. Reproduction, September 1, 2006; 132(3): 413 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Whitaker Calcium at Fertilization and in Early Development Physiol Rev, January 1, 2006; 86(1): 25 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M Mehlmann and L. A Jaffe SH2 domain-mediated activation of an SRC family kinase is not required to initiate Ca2+ release at fertilization in mouse eggs Reproduction, May 1, 2005; 129(5): 557 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. O'Neill, J. Gillett, and K. R. Foltz Distinct roles for multiple Src family kinases at fertilization J. Cell Sci., December 1, 2004; 117(25): 6227 - 6238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kurokawa, K.-i. Sato, J. Smyth, H. Wu, K. Fukami, T. Takenawa, and R. A Fissore Evidence that activation of Src family kinase is not required for fertilization-associated [Ca2+]i oscillations in mouse eggs Reproduction, April 1, 2004; 127(4): 441 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Talmor-Cohen, R Tomashov-Matar, E Eliyahu, R Shapiro, and R Shalgi Are Src family kinases involved in cell cycle resumption in rat eggs? Reproduction, April 1, 2004; 127(4): 455 - 463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Spinella, L. Rosano, V. Di Castro, M. R. Nicotra, P. G. Natali, and A. Bagnato Endothelin-1 Decreases Gap Junctional Intercellular Communication by Inducing Phosphorylation of Connexin 43 in Human Ovarian Carcinoma Cells J. Biol. Chem., October 17, 2003; 278(42): 41294 - 41301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-i. Sato, A. A. Tokmakov, C.-L. He, M. Kurokawa, T. Iwasaki, M. Shirouzu, R. A. Fissore, S. Yokoyama, and Y. Fukami Reconstitution of Src-dependent Phospholipase C{gamma} Phosphorylation and Transient Calcium Release by Using Membrane Rafts and Cell-free Extracts from Xenopus Eggs J. Biol. Chem., October 3, 2003; 278(40): 38413 - 38420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Leckie, R. Empson, A. Becchetti, J. Thomas, A. Galione, and M. Whitaker The NO Pathway Acts Late during the Fertilization Response in Sea Urchin Eggs J. Biol. Chem., March 28, 2003; 278(14): 12247 - 12254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Tunquist and J. L. Maller Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs Genes & Dev., March 15, 2003; 17(6): 683 - 710. [Full Text] [PDF]
|
|
|
|
 |
|
 K.-i. Sato, T. Iwasaki, K. Ogawa, M. Konishi, A. A. Tokmakov, and Y. Fukami Low density detergent-insoluble membrane of Xenopus eggs: subcellular microdomain for tyrosine kinase signaling in fertilization Development, March 4, 2003; 129(4): 885 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Borra, F. J. O'Neill, M. D. Jackson, B. Marshall, E. Verdin, K. R. Foltz, and J. M. Denu Conserved Enzymatic Production and Biological Effect of O-Acetyl-ADP-ribose by Silent Information Regulator 2-like NAD+-dependent Deacetylases J. Biol. Chem., April 5, 2002; 277(15): 12632 - 12641. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Carafoli Calcium signaling: A tale for all seasons PNAS, February 5, 2002; 99(3): 1115 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wu, J. Smyth, V. Luzzi, K. Fukami, T. Takenawa, S. L. Black, N. L. Allbritton, and R. A. Fissore Sperm Factor Induces Intracellular Free Calcium Oscillations by Stimulating the Phosphoinositide Pathway Biol Reprod, May 1, 2001; 64(5): 1338 - 1349. [Abstract] [Full Text]
|
|
|
|
|
|
L. Runft and L. Jaffe Sperm extract injection into ascidian eggs signals Ca(2+) release by the same pathway as fertilization Development, January 8, 2000; 127(15): 3227 - 3236. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |