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J. Biol. Chem., Vol. 275, Issue 22, 16788-16794, June 2, 2000
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From the Recent evidence has indicated a requirement for a
Src family kinase in initiating Ca2+ release at
fertilization in starfish eggs (Giusti, A. F., Carroll, D. J., Abassi, Y. A., Terasaki, M., Foltz, K. R., and Jaffe,
L. A. (1999) J. Biol. Chem. 274, 29318-29322).
We now show that injection of Src protein into starfish eggs initiates
Ca2+ release and DNA synthesis, as occur at fertilization.
These responses depend on the phosphorylation state of the Src protein;
only the kinase active form is effective. Like Ca2+ release
at fertilization, the Ca2+ release in response to Src
protein injection is inhibited by prior injection of the SH2 domains of
phospholipase C
Evidence That Fertilization Activates Starfish Eggs by Sequential
Activation of a Src-like Kinase and Phospholipase C
*
§,
§,
§, and
§¶
Marine Biological Laboratory, Woods Hole,
Massachusetts 02543, the § Department of Physiology,
University of Connecticut Health Center, Farmington, Connecticut 06032, and the ¶ Department of Biological Structure, Biomolecular
Structure Center, University of Washington,
Seattle, Washington 98195
. These findings support the conclusion that in
starfish, sperm-egg interaction causes egg activation by sequential
activation of a Src-like kinase and phospholipase C
. Injection of
the SH2 domain of Src, which inhibits Ca2+ release at
fertilization, does not inhibit Ca2+ release caused by Src
protein injection. This indicates that the requirement for a Src SH2
domain interaction is upstream of Src activation in the pathway leading
to Ca2+ release at fertilization.
*
This work was supported by grants from the National
Institutes of Health (to A. G. and L. A. J.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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