JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Biol. Chem., Vol. 275, Issue 22, 16810-16819, June 2, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vairapandi, M.
Right arrow Articles by Liebermann, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vairapandi, M.
Right arrow Articles by Liebermann, D. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Characterization of MyD118, Gadd45, and Proliferating Cell Nuclear Antigen (PCNA) Interacting Domains
PCNA IMPEDES MyD118 AND Gadd45-MEDIATED NEGATIVE GROWTH CONTROL*

Mariappan Vairapandi, Naiyer Azam, Arthur G. Balliet, Barbara HoffmanDagger , and Dan A. LiebermannDagger

From the Fels Institute for Cancer Research and Molecular Biology and the Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

MyD118 and Gadd45 are related genes encoding for proteins that play important roles in negative growth control, including growth suppression and apoptosis. MyD118 and Gadd45 are related proteins that previously were shown to interact with proliferating cell nuclear antigen (PCNA), implicated in DNA replication, DNA repair, and cell cycle progression. To establish the role of MyD118 and Gadd45 interactions with PCNA, in this work we sought to identify the interacting domains and analyze the significance of this interaction in negative growth control. Using complementary in vivo and in vitro interaction assays the N-terminal (1-46) and middle (100-127) regions of PCNA were identified as harboring MyD118- and Gadd45 interacting domains, whereas PCNA interacting domains within MyD118 and Gadd45 were localized to the C termini of these proteins (amino acids 114-156 and 137-165, respectively). These findings provide first evidence that similar domains within MyD118 and Gadd45 mediate interactions with PCNA. Importantly, ectopic expression of MyD118 or Gadd45 N-terminal peptides, lacking the PCNA interacting domain, was found to suppress colony formation or induce apoptosis more efficiently than the full-length proteins. These findings suggest that interaction of MyD118 or Gadd45 with PCNA, in essence, serves to impede negative growth control.

* This research was supported by National Institutes of Health Grant 1RO1CA43618 (to D. A. L.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence may be addressed: Fels Inst. for Cancer Research and Molecular Biology and the Dept. of Biochemistry, Temple University School of Medicine, 3307 N. Broad St., Philadelphia, Pennsylvania 19140. Tel.: 215-707-6903/2; Fax: 215-707-2805; E-mail: lieberma@unix.temple.edu or hoffman@unix.temple.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. D. Schrag, S. Jiralerspong, M. Banville, M. L. Jaramillo, and M. D. O'Connor-McCourt
The crystal structure and dimerization interface of GADD45{gamma}
PNAS, May 6, 2008; 105(18): 6566 - 6571.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Z. Miyake, M. Takekawa, Q. Ge, and H. Saito
Activation of MTK1/MEKK4 by GADD45 through Induced N-C Dissociation and Dimerization-Mediated trans Autophosphorylation of the MTK1 Kinase Domain
Mol. Cell. Biol., April 1, 2007; 27(7): 2765 - 2776.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
M. Ivanga, Y. Labrie, E. Calvo, P. Belleau, C. Martel, V. Luu-The, J. Morissette, F. Labrie, and F. Durocher
Temporal analysis of E2 transcriptional induction of PTP and MKP and downregulation of IGF-I pathway key components in the mouse uterus
Physiol Genomics, March 14, 2007; 29(1): 13 - 23.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Kawahara, Y.-S. Che, R. Hanaoka, H. Takeda, and I. B. Dawid
Zebrafish GADD45{beta} genes are involved in somite segmentation
PNAS, January 11, 2005; 102(2): 361 - 366.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Papa, F. Zazzeroni, C. G. Pham, C. Bubici, and G. Franzoso
Linking JNK signaling to NF-{kappa}B: a key to survival
J. Cell Sci., October 15, 2004; 117(22): 5197 - 5208.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. K. Mak and D. Kultz
Gadd45 Proteins Induce G2/M Arrest and Modulate Apoptosis in Kidney Cells Exposed to Hyperosmotic Stress
J. Biol. Chem., September 10, 2004; 279(37): 39075 - 39084.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. B. Major and D. A. Jones
Identification of a gadd45{beta} 3' Enhancer That Mediates SMAD3- and SMAD4-dependent Transcriptional Induction by Transforming Growth Factor {beta}
J. Biol. Chem., February 13, 2004; 279(7): 5278 - 5287.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Dong, S.-C. Lyu, A. M. Krensky, and C. Clayberger
DQ 65-79, A Peptide Derived from HLA Class II, Mimics p21 to Block T Cell Proliferation
J. Immunol., November 15, 2003; 171(10): 5064 - 5070.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
H. K. Chung, Y.-W. Yi, N.-C. Jung, D. Kim, J. M. Suh, H. Kim, K. C. Park, D. W. Kim, E. S. Hwang, J. H. Song, et al.
Gadd45{gamma} Expression Is Reduced in Anaplastic Thyroid Cancer and Its Reexpression Results in Apoptosis
J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3913 - 3920.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G. Maga and U. Hubscher
Proliferating cell nuclear antigen (PCNA): a dancer with many partners
J. Cell Sci., August 1, 2003; 116(15): 3051 - 3060.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
H. Zeng and C. D. Davis
Down-Regulation of Proliferating Cell Nuclear Antigen Gene Expression Occurs during Cell Cycle Arrest Induced by Human Fecal Water in Colonic HT-29 Cells
J. Nutr., August 1, 2003; 133(8): 2682 - 2687.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. K. Chung, Y.-W. Yi, N.-C. Jung, D. Kim, J. M. Suh, H. Kim, K. C. Park, J. H. Song, D. W. Kim, E. S. Hwang, et al.
CR6-interacting Factor 1 Interacts with Gadd45 Family Proteins and Modulates the Cell Cycle
J. Biol. Chem., July 18, 2003; 278(30): 28079 - 28088.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. S. Kedar, S.-J. Kim, A. Robertson, E. Hou, R. Prasad, J. K. Horton, and S. H. Wilson
Direct Interaction between Mammalian DNA Polymerase beta and Proliferating Cell Nuclear Antigen
J. Biol. Chem., August 16, 2002; 277(34): 31115 - 31123.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
X. Zhang, H. Sun, D. C. Danila, S. R. Johnson, Y. Zhou, B. Swearingen, and A. Klibanski
Loss of Expression of GADD45{gamma}, a Growth Inhibitory Gene, in Human Pituitary Adenomas: Implications for Tumorigenesis
J. Clin. Endocrinol. Metab., March 1, 2002; 87(3): 1262 - 1267.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
R. Hoffmann, T. Seidl, M. Neeb, A. Rolink, and F. Melchers
Changes in Gene Expression Profiles in Developing B Cells of Murine Bone Marrow
Genome Res., January 1, 2002; 12(1): 98 - 111.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Kovalsky, F.-D. T. Lung, P. P. Roller, and A. J. Fornace Jr.
Oligomerization of Human Gadd45a Protein
J. Biol. Chem., October 12, 2001; 276(42): 39330 - 39339.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Scott, P. Bonnefin, D. Vieyra, F.-M. Boisvert, D. Young, D. P. Bazett-Jones, and K. Riabowol
UV-induced binding of ING1 to PCNA regulates the induction of apoptosis
J. Cell Sci., January 10, 2001; 114(19): 3455 - 3462.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Azam, M. Vairapandi, W. Zhang, B. Hoffman, and D. A. Liebermann
Interaction of CR6 (GADD45gamma ) with Proliferating Cell Nuclear Antigen Impedes Negative Growth Control
J. Biol. Chem., January 19, 2001; 276(4): 2766 - 2774.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.