HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 22, 17016-17023, June 2, 2000

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Okada, Y. Articles by Nagashima, K. Search for Related Content PubMed PubMed Citation Articles by Okada, Y. Articles by Nagashima, K. Social Bookmarking                      What's this?

Transcriptional Activation of JC Virus by Human T-lymphotropic Virus Type I Tax Protein in Human Neuronal Cell Lines^*

From the ^a Laboratory of Molecular & Cellular Pathology, School of Medicine and ^b Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo 060-8638, ^c CREST, Japan Science and Technology, ^f Department of Pathology, Sapporo Municipal Hospital, Chuo-ku, Sapporo 060-8604, ^g Department of Infectious Pathology, National Institute of Infectious Disease, Shinjuku-ku, Tokyo 162-0052, ^h Department of Microbiology, Kansai Medical University, Fumizono-cho, Moriguchi, Osaka 570-8506, ⁱ Department of Infectious Disease and Immunology, University of Ryukyu, Nishihara-cho, Okinawa 903-0125, Japan, and ^j Department of Medical Microbiology, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland

Polyomavirus JC (JCV) causes the human demyelinating disease, progressive multifocal leukoencephalopathy (PML). The recent demonstration of cases of PML in association with human T-lymphotropic virus type I (HTLV-I) infection prompted us to examine whether the HTLV-I-encoded regulatory protein Tax activates JCV transcription. By employing a dual luciferase assay, we initially found that the expression of Tax activated the transcriptional potential of both early and late promoters of JCV in human neuronal but not in non-neuronal cells. We subsequently analyzed the mechanism of Tax-induced activation of the JCV promoter in neuronal cells with the following results: 1) the JCV promoter that lacks the NF-B-binding motif could not be activated by Tax; 2) the overexpression of IB abolished Tax-induced transcriptional activation of the JCV promoter; 3) a Tax mutant (M22) lacking the potential for activation via the NF-B pathway did not activate the JCV promoter. Furthermore, Tax enhances the gene expression of JCV T antigen and VP1. We examined mechanisms of the cell-specific activation of the JCV promoter by Tax. Electrophoretic mobility shift assay demonstrated the presence of Tax-bound protein(s) that were specifically present in non-neuronal cells. This study is the first demonstration of the activation of JCV promoter by HTLV-I Tax in an NF-B-dependent manner.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				K. Manley, B. A. O'Hara, G. V. Gee, C. P. Simkevich, J. M. Sedivy, and W. J. Atwood NFAT4 Is Required for JC Virus Infection of Glial Cells J. Virol., December 15, 2006; 80(24): 12079 - 12085. [Abstract] [Full Text] [PDF]

				K. Tabu, A. Ohnishi, Y. Sunden, T. Suzuki, M. Tsuda, S. Tanaka, T. Sakai, K. Nagashima, and H. Sawa A novel function of OLIG2 to suppress human glial tumor cell growth via p27Kip1 transactivation. J. Cell Sci., April 1, 2006; 119(Pt 7): 1433 - 1441. [Abstract] [Full Text] [PDF]

				T. Suzuki, Y. Okada, S. Semba, Y. Orba, S. Yamanouchi, S. Endo, S. Tanaka, T. Fujita, S. Kuroda, K. Nagashima, et al. Identification of FEZ1 as a Protein That Interacts with JC Virus Agnoprotein and Microtubules: ROLE OF AGNOPROTEIN-INDUCED DISSOCIATION OF FEZ1 FROM MICROTUBULES IN VIRAL PROPAGATION J. Biol. Chem., July 1, 2005; 280(26): 24948 - 24956. [Abstract] [Full Text] [PDF]

				Q. Qu, H. Sawa, T. Suzuki, S. Semba, C. Henmi, Y. Okada, M. Tsuda, S. Tanaka, W. J. Atwood, and K. Nagashima Nuclear Entry Mechanism of the Human Polyomavirus JC Virus-like Particle: ROLE OF IMPORTINS AND THE NUCLEAR PORE COMPLEX J. Biol. Chem., June 25, 2004; 279(26): 27735 - 27742. [Abstract] [Full Text] [PDF]

				R. Komagome, H. Sawa, T. Suzuki, Y. Suzuki, S. Tanaka, W. J. Atwood, and K. Nagashima Oligosaccharides as Receptors for JC Virus J. Virol., November 13, 2002; 76(24): 12992 - 13000. [Abstract] [Full Text] [PDF]