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Originally published In Press as doi:10.1074/jbc.M001635200 on April 4, 2000

J. Biol. Chem., Vol. 275, Issue 23, 17391-17398, June 9, 2000
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Identification of a Yeast Transcription Factor IID Subunit, TSG2/TAF48*

Joseph C. ReeseDagger , Zhengjian Zhang, and Harsha Kurpad

From the Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802

The RNA polymerase II general transcription factor TFIID is a complex containing the TATA-binding protein (TBP) and associated factors (TAFs). We have used a mutant allele of the gene encoding yeast TAFII68/61p to analyze its function in vivo. We provide biochemical and genetic evidence that the C-terminal alpha -helix of TAFII68/61p is required for its direct interaction with TBP, the stable incorporation of TBP into the TFIID complex, the integrity of the TFIID complex, and the transcription of most genes in vivo. This is the first evidence that a yeast TAFII other than TAFII145/130 interacts with TBP, and the implications of this on the interpretation of data obtained studying TAFII mutants in vivo are discussed. We have identified a high copy suppressor of the TAF68/61 mutation, TSG2, that has sequence similarity to a region of the SAGA subunit Ada1. We demonstrate that it directly interacts with TAFII68/61p in vitro, is a component of TFIID, is required for the stability of the complex in vivo, and is necessary for the transcription of many yeast genes. On the basis of these functions, we propose that Tsg2/TAFII48p is the histone 2A-like dimerization partner for the histone 2B-like TAFII68/61p in the yeast TFIID complex.

* This work was supported in part National Institutes of Health Grant GM58672 (to J. C. R.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, Pennsylvania State University, 203 Althouse Laboratory, University Park, PA 16802-4500. Tel.: 814-865-1976; Fax: 814-863-7024; E-mail: jcr8@psu.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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