|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 23, 17412-17419, June 9, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Physiology and Biophysics, Finch University
of Health Sciences/The Chicago Medical School,
North Chicago, Illinois 60064
Recently, several mammalian K+
channel subunits (TWIK, TREK-1, TRAAK, and TASK) possessing four
transmembrane segments and two pore-forming domains have been
identified. We report the cloning of a new member of this tandem-pore
K+ channel from a rat cerebellum cDNA library. It is a
538-amino acid protein and shares 65% amino acid sequence identity
with TREK-1. Therefore, the new clone was named TREK-2. Unlike TREK-1, whose mRNA has been reported to be expressed in many different tissues, TREK-2 mRNA is expressed mainly in the cerebellum, spleen, and testis as judged by reverse transcriptase-polymerase chain reaction
and Northern blot analysis. Expression of TREK-2 in COS-7 cells induced
a time-independent and non-inactivating K+-selective
current. TREK-2 was partially blocked (36%) by 2 mM Ba2+. In symmetrical 150 mM KCl, the
single-channel conductances were 110 picosiemens at The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF196965.
TREK-2, a New Member of the Mechanosensitive Tandem-pore
K+ Channel Family*
,
40 mV and 68 picosiemens at +40 mV, and the mean open time was 0.9 ms at 40 mV.
TREK-2 was activated by membrane stretch or acidic pH. At 40 mm Hg
pressure, channel activity increased 10-fold above the basal level.
TREK-2 was also activated by arachidonic acid and other naturally
occurring unsaturated free fatty acids. These results show that TREK-2
is a new member of the tandem-pore K+ channel family and
belongs to the class of mechanosensitive and fatty acid-stimulated
K+ channels.
*
This work was supported by a grant-in-aid from the American
Heart Association.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Dept. of Physiology, Chung-Ang University College
of Medicine, Seoul 156-756, Korea.
§
To whom correspondence should be addressed: Dept. of Physiology and
Biophysics, Finch University of Health Sciences/The Chicago Medical
School, 3333 Green Bay Rd., North Chicago, IL 60064. Tel.: 847-578-3280; Fax: 847-578-3265; E-mail:
donghee.kim@finchcms.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Zheng, J. H. Nam, B. Pang, D. H. Shin, J. S. Kim, Y.-S. Chun, J.-W. Park, H. Bang, W. K. Kim, Y. E. Earm, et al. Identification of the large-conductance background K+ channel in mouse B cells as TREK-2 Am J Physiol Cell Physiol, July 1, 2009; 297(1): C188 - C197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Xiao, P.-Y. Deng, L. Rojanathammanee, C. Yang, L. Grisanti, K. Permpoonputtana, D. Weinshenker, V. A. Doze, J. E. Porter, and S. Lei Noradrenergic Depression of Neuronal Excitability in the Entorhinal Cortex via Activation of TREK-2 K+ Channels J. Biol. Chem., April 17, 2009; 284(16): 10980 - 10991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Simkin, E. J. Cavanaugh, and D. Kim Control of the single channel conductance of K2P10.1 (TREK-2) by the amino-terminus: role of alternative translation initiation J. Physiol., December 1, 2008; 586(23): 5651 - 5663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Sandoz, M. P. Tardy, S. Thummler, S. Feliciangeli, M. Lazdunski, and F. Lesage Mtap2 Is a Constituent of the Protein Network That Regulates Twik-Related K+ Channel Expression and Trafficking J. Neurosci., August 20, 2008; 28(34): 8545 - 8552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Heitzmann and R. Warth Physiology and Pathophysiology of Potassium Channels in Gastrointestinal Epithelia Physiol Rev, July 1, 2008; 88(3): 1119 - 1182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pokojski, C. Busch, I. Grgic, M. Kacik, W. Salman, R. Preisig-Muller, W.-T. Heyken, J. Daut, J. Hoyer, and R. Kohler TWIK-related two-pore domain potassium channel TREK-1 in carotid endothelium of normotensive and hypertensive mice Cardiovasc Res, April 3, 2008; (2008) cvn069v2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kang, C. Choe, E. Cavanaugh, and D. Kim Properties of single two-pore domain TREK-2 channels expressed in mammalian cells J. Physiol., August 15, 2007; 583(1): 57 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mathie Neuronal two-pore-domain potassium channels and their regulation by G protein-coupled receptors J. Physiol., January 15, 2007; 578(2): 377 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kang, J. Han, and D. Kim Mechanism of inhibition of TREK-2 (K2P10.1) by the Gq-coupled M3 muscarinic receptor Am J Physiol Cell Physiol, October 1, 2006; 291(4): C649 - C656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Brock, M. C. Acosta, A. Al Abed, S. Pianova, and C. Belmonte Barium ions inhibit the dynamic response of guinea-pig corneal cold receptors to heating but not to cooling J. Physiol., September 1, 2006; 575(2): 573 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Bryan Jr., J. You, S. C. Phillips, J. J. Andresen, E. E. Lloyd, P. A. Rogers, S. E. Dryer, and S. P. Marrelli Evidence for two-pore domain potassium channels in rat cerebral arteries Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H770 - H780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Honore, A. J. Patel, J. Chemin, T. Suchyna, and F. Sachs Desensitization of mechano-gated K2P channels PNAS, May 2, 2006; 103(18): 6859 - 6864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Sanders and S. Don Koh Two-pore-domain potassium channels in smooth muscles: new components of myogenic regulation J. Physiol., January 1, 2006; 570(1): 37 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Taverna, T. Tkatch, A. E. Metz, and M. Martina Differential Expression of TASK Channels between Horizontal Interneurons and Pyramidal Cells of Rat Hippocampus J. Neurosci., October 5, 2005; 25(40): 9162 - 9170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Enyeart, S. J. Danthi, H. Liu, and J. A. Enyeart Angiotensin II Inhibits bTREK-1 K+ Channels in Adrenocortical Cells by Separate Ca2+- and ATP Hydrolysis-dependent Mechanisms J. Biol. Chem., September 2, 2005; 280(35): 30814 - 30828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Kim, J.-Y. Park, H.-W. Kang, E.-J. Lee, H. Bang, and J.-H. Lee Zinc Activates TREK-2 Potassium Channel Activity J. Pharmacol. Exp. Ther., August 1, 2005; 314(2): 618 - 625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Bai, G. J Bugg, S. L Greenwood, J. D Glazier, C. P Sibley, P. N Baker, M. J Taggart, and G. K Fyfe Expression of TASK and TREK, two-pore domain K+ channels, in human myometrium Reproduction, April 1, 2005; 129(4): 525 - 530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kang, C. Choe, and D. Kim Thermosensitivity of the two-pore domain K+ channels TREK-2 and TRAAK J. Physiol., April 1, 2005; 564(1): 103 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chemin, A. Patel, F. Duprat, M. Zanzouri, M. Lazdunski, and E. Honore Lysophosphatidic Acid-operated K+ Channels J. Biol. Chem., February 11, 2005; 280(6): 4415 - 4421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Bai, S. L. Greenwood, J. D. Glazier, P. N. Baker, C. P. Sibley, M. J. Taggart, and G. K. Fyfe Localization of TASK and TREK, Two-Pore Domain K+ Channels, in Human Cytotrophoblast Cells Reproductive Sciences, February 1, 2005; 12(2): 77 - 83. [Abstract] [PDF]
|
|
|
|
|
|
S. C. Hebert, G. Desir, G. Giebisch, and W. Wang Molecular Diversity and Regulation of Renal Potassium Channels Physiol Rev, January 1, 2005; 85(1): 319 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Duprat, C. Girard, G. Jarretou, and M. Lazdunski Pancreatic two P domain K+ channels TALK-1 and TALK-2 are activated by nitric oxide and reactive oxygen species J. Physiol., January 1, 2005; 562(1): 235 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. Ander, A. R. Weber, P. P. Rampersad, J. S. C. Gilchrist, G. N. Pierce, and A. Lukas Dietary Flaxseed Protects against Ventricular Fibrillation Induced by Ischemia-Reperfusion in Normal and Hypercholesterolemic Rabbits J. Nutr., December 1, 2004; 134(12): 3250 - 3256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kang, E. Mariash, and D. Kim Functional Expression of TRESK-2, a New Member of the Tandem-pore K+ Channel Family J. Biol. Chem., July 2, 2004; 279(27): 28063 - 28070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Nam, J.-E. Woo, D.-Y. Uhm, and S. J. Kim Membrane-delimited Regulation of Novel Background K+ Channels by MgATP in Murine Immature B Cells J. Biol. Chem., May 14, 2004; 279(20): 20643 - 20654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Czirjak, Z. E. Toth, and P. Enyedi The Two-pore Domain K+ Channel, TRESK, Is Activated by the Cytoplasmic Calcium Signal through Calcineurin J. Biol. Chem., April 30, 2004; 279(18): 18550 - 18558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Han, D. Kang, and D. Kim Functional properties of four splice variants of a human pancreatic tandem-pore K+ channel, TALK-1 Am J Physiol Cell Physiol, September 1, 2003; 285(3): C529 - C538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sano, K. Inamura, A. Miyake, S. Mochizuki, C. Kitada, H. Yokoi, K. Nozawa, H. Okada, H. Matsushime, and K. Furuichi A Novel Two-pore Domain K+ Channel, TRESK, Is Localized in the Spinal Cord J. Biol. Chem., July 18, 2003; 278(30): 27406 - 27412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Miller, P J Kemp, A Lewis, C G Chapman, H J Meadows, and C Peers Acute hypoxia occludes hTREK-1 modulation: re-evaluation of the potential role of tandem P domain K+ channels in central neuroprotection J. Physiol., April 1, 2003; 548(1): 31 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Talley, J. E. Sirois, Q. Lei, and D. A. Bayliss Two-Pore-Domain (Kcnk) Potassium Channels: Dynamic Roles in Neuronal Function Neuroscientist, February 1, 2003; 9(1): 46 - 56. [Abstract] [PDF]
|
|
|
|
|
|
J. Han, C. Gnatenco, C. D Sladek, and D. Kim Background and tandem-pore potassium channels in magnocellular neurosecretory cells of the rat supraoptic nucleus J. Physiol., February 1, 2003; 546(3): 625 - 639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Enyeart, L. Xu, S. Danthi, and J. A. Enyeart An ACTH- and ATP-regulated Background K+ Channel in Adrenocortical Cells Is TREK-1 J. Biol. Chem., December 13, 2002; 277(51): 49186 - 49199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J Allen and D. Attwell Modulation of ASIC channels in rat cerebellar purkinje neurons by ischaemia-related signals J. Physiol., September 1, 2002; 543(2): 521 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Lyford, P. R. Strege, A. Shepard, Y. Ou, L. Ermilov, S. M. Miller, S. J. Gibbons, J. L. Rae, J. H. Szurszewski, and G. Farrugia alpha 1C (CaV1.2) L-type calcium channel mediates mechanosensitive calcium regulation Am J Physiol Cell Physiol, September 1, 2002; 283(3): C1001 - C1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Han, J. Truell, C. Gnatenco, and D. Kim Characterization of four types of background potassium channels in rat cerebellar granule neurons J. Physiol., July 15, 2002; 542(2): 431 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Gu, G. Schlichthorl, J. R Hirsch, H. Engels, C. Karschin, A. Karschin, C. Derst, O. K Steinlein, and J. Daut Expression pattern and functional characteristics of two novel splice variants of the two-pore-domain potassium channel TREK-2 J. Physiol., March 15, 2002; 539(3): 657 - 668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Talley, G. Solorzano, Q. Lei, D. Kim, and D. A. Bayliss CNS Distribution of Members of the Two-Pore-Domain (KCNK) Potassium Channel Family J. Neurosci., October 1, 2001; 21(19): 7491 - 7505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. P. Hamill and B. Martinac Molecular Basis of Mechanotransduction in Living Cells Physiol Rev, April 1, 2001; 81(2): 685 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Lesage, C. Terrenoire, G. Romey, and M. Lazdunski Human TREK2, a 2P Domain Mechano-sensitive K+ Channel with Multiple Regulations by Polyunsaturated Fatty Acids, Lysophospholipids, and Gs, Gi, and Gq Protein-coupled Receptors J. Biol. Chem., September 8, 2000; 275(37): 28398 - 28405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Patel, F. Maingret, V. Magnone, M. Fosset, M. Lazdunski, and E. Honore TWIK-2, an Inactivating 2P Domain K+ Channel J. Biol. Chem., September 8, 2000; 275(37): 28722 - 28730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rajan, E. Wischmeyer, C. Karschin, R. Preisig-Muller, K.-H. Grzeschik, J. Daut, A. Karschin, and C. Derst THIK-1 and THIK-2, a Novel Subfamily of Tandem Pore Domain K+ Channels J. Biol. Chem., March 2, 2001; 276(10): 7302 - 7311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Czirjak and P. Enyedi Formation of Functional Heterodimers between the TASK-1 and TASK-3 Two-pore Domain Potassium Channel Subunits J. Biol. Chem., February 8, 2002; 277(7): 5426 - 5432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Gu, G. Schlichthorl, J. R. Hirsch, H. Engels, C. Karschin, A. Karschin, C. Derst, O. K. Steinlein, and J. Daut Expression pattern and functional characteristics of two novel splice variants of the two-pore-domain potassium channel TREK-2 J. Physiol., February 8, 2002; (2002) 200101343. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |