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J. Biol. Chem., Vol. 275, Issue 23, 17619-17625, June 9, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/23/17619    most recent M000647200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Locatelli, F. Articles by Coraggio, I. Search for Related Content PubMed PubMed Citation Articles by Locatelli, F. Articles by Coraggio, I. Social Bookmarking                      What's this?

The Product of the Rice myb7 Unspliced mRNA Dimerizes with the Maize Leucine Zipper Opaque2 and Stimulates Its Activity in a Transient Expression Assay^*

Franca Locatelli, Marcella Bracale, Flavio Magaraggia, Franco Faoro^¶, Lucia A. Manzocchi, and Immacolata Coraggio

From the Istituto Biosintesi Vegetali, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano the ^¶ Centro Miglioramento Sanitario Colture Agrarie, Consiglio Nazionale delle Ricerche, via Celoria 2, Milano 20133 and the Dipartimento di Bologia Strutturale e Funzionale, Università dell' Insubria, via J. H. Dunant 3, Varese 21100, Italy

myb7 mRNA is present in rice in spliced and unspliced forms, splicing being enhanced by anoxia. The protein (Mybleu) encoded by the unspliced mRNA is composed of an incomplete Myb domain followed by a leucine zipper; however, it lacks canonical sequences for DNA binding, transcriptional activation, and nuclear localization. We show here that in transiently transformed tobacco protoplasts, Mybleu is able to enhance the transcriptional activity of the maize leucine zipper Opaque2 on its target b32 promoter. The Mybleu transactivation effect is strictly dependent on the presence of Opaque2 and is driven by Mybleu-Opaque2 heterodimers. Mybleu is located in the nucleus, both in rice and in transformed tobacco protoplasts. In rice, the protein is expressed in regions corresponding to undifferentiated cells of roots and coleoptiles. Therefore, myb7 mRNA encodes, depending on its splicing, two transcription factors belonging to separate classes. One of them, Mybleu, has novel structural characteristics, suggesting the existence of new mechanisms acting in the activation of transcription.

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