HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 24, 17925-17928, June 16, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/24/17925    most recent C000146200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chan, S.-L. Articles by Yu, V. C. Search for Related Content PubMed PubMed Citation Articles by Chan, S.-L. Articles by Yu, V. C. Social Bookmarking                      What's this?

ACCELERATED PUBLICATION
The Caenorhabditis elegans Sex Determination Protein FEM-1 Is a CED-3 Substrate That Associates with CED-4 and Mediates Apoptosis in Mammalian Cells^*

Shing-Leng Chan, Karen S. Y. Yee, Karen Mei Ling Tan, and Victor C. Yu^§

From the Institute of Molecular and Cell Biology, 30 Medical Dr., Singapore 117609, Republic of Singapore

Sex-specific elimination of cells by apoptosis plays a role in sex determination in Caenorhabditis elegans. Recently, a mammalian pro-apoptotic protein named F1A has been identified. F1A shares extensive homology throughout the entire protein with the C. elegans protein, FEM-1, which is essential for achieving all aspects of the male phenotype in the nematode. In this report, the role of FEM-1 in apoptosis was investigated. Overexpression of FEM-1 induces caspase-dependent apoptosis in mammalian cells. FEM-1 is cleaved in vitro by the C. elegans caspase, CED-3, generating an N-terminal cleavage product that corresponds to the minimal effector domain for apoptosis. Furthermore, CED-4 associates with FEM-1 in vitro and in vivo in mammalian cells and potentiates FEM-1-mediated apoptosis. Similarly, Apaf-1, the mammalian homologue of CED-4 was found to associate with F1A. These data suggest that FEM-1 and F1A may mediate apoptosis by communicating directly with the core machinery of apoptosis.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				V. Roukos, M. S. Iliou, H. Nishitani, M. Gentzel, M. Wilm, S. Taraviras, and Z. Lygerou Geminin Cleavage during Apoptosis by Caspase-3 Alters Its Binding Ability to the SWI/SNF Subunit Brahma J. Biol. Chem., March 30, 2007; 282(13): 9346 - 9357. [Abstract] [Full Text] [PDF]

				J. Oyhenart, S. Benichou, and N. Raich Putative Homeodomain Transcription Factor 1 Interacts with the Feminization Factor Homolog Fem1b in Male Germ Cells Biol Reprod, April 1, 2005; 72(4): 780 - 787. [Abstract] [Full Text] [PDF]

				C. L. Schlamp, A. T. Thliveris, Y. Li, L. P. Kohl, C. Knop, J. A. Dietz, I. V. Larsen, P. Imesch, L. H. Pinto, and R. W. Nickells Insertion of the {beta}Geo Promoter Trap into the Fem1c Gene of ROSA3 Mice Mol. Cell. Biol., May 1, 2004; 24(9): 3794 - 3803. [Abstract] [Full Text] [PDF]

				J. M. Shulman and M. B. Feany Genetic Modifiers of Tauopathy in Drosophila Genetics, November 1, 2003; 165(3): 1233 - 1242. [Abstract] [Full Text] [PDF]

				K. M. L. Tan, S.-L. Chan, K. O. Tan, and V. C. Yu The Caenorhabditis elegans Sex-determining Protein FEM-2 and Its Human Homologue, hFEM-2, Are Ca2+/Calmodulin-dependent Protein Kinase Phosphatases That Promote Apoptosis J. Biol. Chem., November 16, 2001; 276(47): 44193 - 44202. [Abstract] [Full Text] [PDF]

				K. O. Tan, K. M. L. Tan, S.-L. Chan, K. S. Y. Yee, M. Bevort, K. C. Ang, and V. C. Yu MAP-1, a Novel Proapoptotic Protein Containing a BH3-like Motif That Associates with Bax through Its Bcl-2 Homology Domains J. Biol. Chem., January 19, 2001; 276(4): 2802 - 2807. [Abstract] [Full Text] [PDF]