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J. Biol. Chem., Vol. 275, Issue 25, 18845-18850, June 23, 2000
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From the Laboratoire de Pharmacologie et de Biologie Structurale,
CNRS UMR 5089, 205 route de Narbonne,
31077 Toulouse Cedex, France
The first processing event of the precursor
ribosomal RNA (pre-rRNA) takes place within the 5' external transcribed
spacer. This primary processing requires conserved
cis-acting RNA sequence downstream from the cleavage site
and several nucleic acids (small nucleolar RNAs) and proteins
trans-acting factors including nucleolin, a major nucleolar protein.
The specific interaction of nucleolin with the pre-rRNA is required for
processing in vitro. Xenopus laevis and hamster nucleolin
interact with the same pre-rRNA site and stimulate the processing
activity of a mouse cell extract. A highly conserved 11-nucleotide
sequence located 5-6 nucleotides after the processing site is required
for the interaction of nucleolin and processing. In vitro
selection experiments with nucleolin have identified an RNA sequence
that contains the UCGA motif present in the 11-nucleotide conserved
sequence. The interaction of nucleolin with pre-rRNA is required for
the formation of an active processing complex. Our findings demonstrate
that nucleolin is a key factor for the assembly and maturation of
pre-ribosomal ribonucleoparticles.
Interaction of Nucleolin with an Evolutionarily Conserved
Pre-ribosomal RNA Sequence Is Required for the Assembly of the
Primary Processing Complex*
*
This work was supported by grants from the Université
Paul Sabatier (Toulouse), the Center National de la Recherche
Scientifique, and the Association pour la Recherche contre le Cancer.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Laboratoire de
Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de
Narbonne, 31077 Toulouse Cedex, France. Tel.: 33-5-61-17-59-51; Fax:
33-5-61-17-59-94; E-mail: bouvet@ipbs.fr.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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