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J. Biol. Chem., Vol. 275, Issue 25, 19224-19230, June 23, 2000
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From the The form I (cbbI)
Calvin-Benson-Bassham (CBB) reductive pentose phosphate cycle operon of
Rhodobacter sphaeroides is regulated by both the
transcriptional activator CbbR and the RegA/PrrA (RegB/PrrB)
two-component signal transduction system. DNase I footprint analyses
indicated that R. sphaeroides CbbR binds to the
cbbI promoter between
Interaction of CbbR and RegA* Transcription Regulators with
the Rhodobacter sphaeroides cbbI Promoter-Operator
Region*
,¶
Department of Microbiology and Plant
Biotechnology Center, The Ohio State University, Columbus, Ohio
43210-1292 and the § Department of Biology, Indiana
University, Bloomington, Indiana 47405
10 and 70 base pairs
(bp) relative to the cbbI transcription start.
A cosmid carrying the R. capsulatus reg locus was capable
of complementing an R. sphaeroides regA-deficient mutant to
phototrophic growth with restored regulated synthesis of both
photopigments and ribulose-bisphosphate carboxylase/oxygenase (Rubisco). DNase I footprint analyses, using R. capsulatus
RegA*, a constitutively active mutant version of RegA, detected four RegA* binding sites within the cbbI promoter.
Two sites were found within a previously identified
cbbI promoter proximal regulatory region from
61 to 110 bp. One of these proximal RegA* binding sites overlapped
that of CbbR. Two sites were within a previously identified promoter
distal positive regulatory region between 301 and 415 bp.
Expression from promoter insertion mutants showed that the function of
the promoter distal regulatory region was helical
phase-dependent. These results indicated that RegA exerts its regulatory affect on cbbI expression
through direct interaction with the cbbI promoter.
*
This work was supported by National Institutes of Health
Grants GM-45404 (to F. R. T.) and GM-40941 (to C. E. B.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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