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Originally published In Press as doi:10.1074/jbc.M000900200 on March 23, 2000

J. Biol. Chem., Vol. 275, Issue 25, 19231-19240, June 23, 2000
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A Protein Containing a Serine-rich Domain with Vesicle Fusing Properties Mediates Cell Cycle-dependent Cytosolic pH Regulation*

Derrick T. BrazillDagger §, David R. Caprette§, Heather A. Myler§, R. Diane HattonDagger , Robin R. AmmannDagger , David F. LindseyDagger ||, Debra A. BrockDagger , and Richard H. GomerDagger §**

From the Dagger  Howard Hughes Medical Institute and the § Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005-1892

Initial differentiation in Dictyostelium involves both asymmetric cell division and a cell cycle-dependent mechanism. We previously identified a gene, rtoA, which when disrupted randomizes the cell cycle-dependent mechanism without affecting either the underlying cell cycle or asymmetric differentiation. We find that in wild-type cells, RtoA levels vary during the cell cycle. Cytosolic pH, which normally varies with the cell cycle, is randomized in rtoA cells. The middle 60% of the RtoA protein is 10 tandem repeats of an 11 peptide-long serine-rich motif, which we find has a random coil structure. This domain catalyzes the fusion of phospholipid vesicles in vitro. Conversely, rtoA cells have a defect in the fusion of endocytic vesicles. They also have a decreased exocytosis rate, a decreased pH of endocytic/exocytic vesicles, and an increased average cytosolic pH. Our data indicate that the serine-rich domain of RtoA can mediate membrane fusion and that RtoA can increase the rate of vesicle fusion during processing of endoctyic vesicles. We hypothesize that RtoA modulates initial cell type choice by linking vegetative cell physiology to the cell cycle.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Dept. of Biology, Hunter College, 695 Park Ave., New York, NY 10021.

|| Present address: Dept. of Biological Sciences, Walla Walla College, College Place, WA 99324.

** Associate investigator of the Howard Hughes Medical Institute. To whom correspondence should be addressed: Howard Hughes Medical Institute, MS-140, Rice University, 6100 S. Main St., Houston, TX 77005-1892. Tel.: 713-348-4872; Fax: 713-348-5154; E-mail: richard@ bioc.rice.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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