| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 25, 19361-19367, June 23, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From INSERM U397, Endocrinologie et Communication Cellulaire,
Institut Louis Bugnard, C.H.U. Rangueil, Avenue Jean Poulhès,
31403 Toulouse Cedex 04, France
Five fibroblast growth factor 2 (FGF-2) isoforms
are synthesized from human FGF-2 mRNA by a process of alternative
initiation of translation. The regulation of FGF-2 isoform expression
by the mRNA 5823-nucleotide-long 3'-untranslated region containing eight alternative polyadenylation sites was examined. Because previous
studies had shown that FGF-2 expression was regulated in primary cells
but not in transformed cells, primary human skin fibroblasts were used
in this study. Using an approach of cell transfection with synthetic
reporter mRNAs, a novel translational enhancer (3'-TE) was
identified in the 1370-nucleotide mRNA segment located upstream
from the eighth poly(A) site. Deletion mutagenesis showed that the
3'-TE was composed of two domains with additive effects. The 3'-TE
exhibited the unique feature of modulating the use of FGF-2 alternative
initiation codons, which favored the relative expression of
CUG-initiated isoforms. Interestingly, the use of an alternative
polydenylation site removing the 3'-TE was detected in skin fibroblasts
in response to heat shock and cell density variations. At high cell
densities, 3'-TE removal was correlated with a loss of CUG-initiated
FGF-2 expression. These data show that the FGF-2 mRNA
3'-untranslated region is able to modulate FGF-2 isoform expression by
the coupled processes of translation activation and alternative polyadenylation.
Alternative Translation Initiation of Human Fibroblast Growth
Factor 2 mRNA Controlled by Its 3'-Untranslated Region Involves a
Poly(A) Switch and a Translational Enhancer*
,
*
This work was supported by grants from the Association pour
la Recherche sur le Cancer, the Ligue Nationale contre le Cancer, and
the Conseil Régional Midi-Pyrénées and by European
Community Biotechnology program (subprogram Cell Factory, Actions de
Recherches Concertées) Grant 94/99-181.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of successive fellowships from the Ministère de
l'Education Nationale et de la Recherche and from the Ligue Nationale contre le Cancer. Present address: Unité de Physiologie
Cellulaire et Moléculaire, CNRS ERS 1590, 31059 Toulouse Cedex, France.
§
Present address: Institut de Pharmacologie et de Biochimie
Structurale, CNRS UPR 9062, 105, route de Narbonne, 31062 Toulouse Cedex, France.
¶
To whom correspondence should be addressed. Tel.:
33-561-32-21-42; Fax: 33-561-32-21-41; E-mail:
pratsac@rangueil.inserm.fr.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Bastide, Z. Karaa, S. Bornes, C. Hieblot, E. Lacazette, H. Prats, and C. Touriol An upstream open reading frame within an IRES controls expression of a specific VEGF-A isoform Nucleic Acids Res., April 1, 2008; 36(7): 2434 - 2445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Fontaine, C. Filipe, N. Werner, P. Gourdy, A. Billon, B. Garmy-Susini, L. Brouchet, F. Bayard, H. Prats, T. Doetschman, et al. Essential Role of Bone Marrow Fibroblast Growth Factor-2 in the Effect of Estradiol on Reendothelialization and Endothelial Progenitor Cell Mobilization Am. J. Pathol., November 1, 2006; 169(5): 1855 - 1862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Lopez, S. Granjeaud, T. Ara, B. Ghattas, and D. Gautheret The disparate nature of "intergenic" polyadenylation sites RNA, October 1, 2006; 12(10): 1794 - 1801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Garmy-Susini, E. Delmas, P. Gourdy, M. Zhou, C. Bossard, B. Bugler, F. Bayard, A. Krust, A.C. Prats, T. Doetschman, et al. Role of Fibroblast Growth Factor-2 Isoforms in the Effect of Estradiol on Endothelial Cell Migration and Proliferation Circ. Res., May 28, 2004; 94(10): 1301 - 1309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bornes, M. Boulard, C. Hieblot, C. Zanibellato, J. S. Iacovoni, H. Prats, and C. Touriol Control of the Vascular Endothelial Growth Factor Internal Ribosome Entry Site (IRES) Activity and Translation Initiation by Alternatively Spliced Coding Sequences J. Biol. Chem., April 30, 2004; 279(18): 18717 - 18726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. V. Skabkina, M. A. Skabkin, N. V. Popova, D. N. Lyabin, L. O. Penalva, and L. P. Ovchinnikov Poly(A)-binding Protein Positively Affects YB-1 mRNA Translation through Specific Interaction with YB-1 mRNA J. Biol. Chem., May 9, 2003; 278(20): 18191 - 18198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Gross, M. G. Callow, L. Waldbaum, S. Thomas, and R. Ruggieri MRK, a Mixed Lineage Kinase-related Molecule That Plays a Role in gamma -Radiation-induced Cell Cycle Arrest J. Biol. Chem., April 12, 2002; 277(16): 13873 - 13882. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
