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Originally published In Press as doi:10.1074/jbc.M001989200 on April 25, 2000

J. Biol. Chem., Vol. 275, Issue 27, 20450-20457, July 7, 2000
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Characterization of a Phosphoinositide-mediated Odor Transduction Pathway Reveals Plasma Membrane Localization of an Inositol 1,4,5-Trisphosphate Receptor in Lobster Olfactory Receptor Neurons*

Steven D. MungerDagger §, Richard A. GleesonDagger , Henry C. Aldrich||, Nicole C. RustDagger , Barry W. AcheDagger §**, and Robert M. GreenbergDagger

From the Dagger  Whitney Laboratory, University of Florida, St. Augustine, Florida 32086, and the Departments of § Neuroscience, || Microbiology and Cell Science, and ** Zoology, University of Florida, Gainesville, Florida 32610

The role of phosphoinositide signaling in olfactory transduction is still being resolved. Compelling functional evidence for the transduction of odor signals via phosphoinositide pathways in olfactory transduction comes from invertebrate olfactory systems, in particular lobster olfactory receptor neurons. We now provide molecular evidence for two components of the phosphoinositide signaling pathway in lobster olfactory receptor neurons, a G protein alpha  subunit of the Gq family and an inositol 1,4,5-trisphosphate-gated channel or an inositol 1,4,5-trisphosphate (IP3) receptor. Both proteins localize to the site of olfactory transduction, the outer dendrite of the olfactory receptor neurons. Furthermore, the IP3 receptor localizes to membranes in the ciliary transduction compartment of these cells at both the light microscopic and electron microscopic levels. Given the absence of intracellular organelles in the sub-micron diameter olfactory cilia, this finding indicates that the IP3 receptor is associated with the plasma membrane and provides the first definitive evidence for plasma membrane localization of an IP3R in neurons. The association of the IP3 receptor with the plasma membrane may be a novel mechanism for regulating intracellular cations in restricted cellular compartments of neurons.

* This work was supported by NIDCD Grant DC01655 (to B. W. A.) from the National Institutes of Health and by National Science Foundation Grant IBN 9604870 (to R. A. G.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AAC61691 and AF201328.

To whom correspondence should be addressed: Dept. Anatomy and Neurobiology, University of Maryland School of Medicine, Health Sciences Facility, Rm. 232, 685 W. Baltimore St., Baltimore, MD 21201. Tel.: 410-706-5851; Fax: 410-706-2512; E-mail: smunger@mail.com.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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