HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 27, 20450-20457, July 7, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/27/20450    most recent M001989200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Munger, S. D. Articles by Greenberg, R. M. Search for Related Content PubMed PubMed Citation Articles by Munger, S. D. Articles by Greenberg, R. M. Social Bookmarking                      What's this?

Characterization of a Phosphoinositide-mediated Odor Transduction Pathway Reveals Plasma Membrane Localization of an Inositol 1,4,5-Trisphosphate Receptor in Lobster Olfactory Receptor Neurons^*

Steven D. Munger^§¶, Richard A. Gleeson, Henry C. Aldrich, Nicole C. Rust, Barry W. Ache^§**, and Robert M. Greenberg

From the  Whitney Laboratory, University of Florida, St. Augustine, Florida 32086, and the Departments of ^§ Neuroscience,  Microbiology and Cell Science, and ^** Zoology, University of Florida, Gainesville, Florida 32610

The role of phosphoinositide signaling in olfactory transduction is still being resolved. Compelling functional evidence for the transduction of odor signals via phosphoinositide pathways in olfactory transduction comes from invertebrate olfactory systems, in particular lobster olfactory receptor neurons. We now provide molecular evidence for two components of the phosphoinositide signaling pathway in lobster olfactory receptor neurons, a G protein  subunit of the G_q family and an inositol 1,4,5-trisphosphate-gated channel or an inositol 1,4,5-trisphosphate (IP₃) receptor. Both proteins localize to the site of olfactory transduction, the outer dendrite of the olfactory receptor neurons. Furthermore, the IP₃ receptor localizes to membranes in the ciliary transduction compartment of these cells at both the light microscopic and electron microscopic levels. Given the absence of intracellular organelles in the sub-micron diameter olfactory cilia, this finding indicates that the IP₃ receptor is associated with the plasma membrane and provides the first definitive evidence for plasma membrane localization of an IP₃R in neurons. The association of the IP₃ receptor with the plasma membrane may be a novel mechanism for regulating intracellular cations in restricted cellular compartments of neurons.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AAC61691 and AF201328.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				Y. Bobkov and B. Ache Block by Amiloride Derivatives of Odor-Evoked Discharge in Lobster Olfactory Receptor Neurons through Action on a Presumptive TRP Channel Chem Senses, February 1, 2007; 32(2): 149 - 159. [Abstract] [Full Text] [PDF]

				R. Stepanyan, K. Day, J. Urban, D. L. Hardin, R. S. Shetty, C. D. Derby, B. W. Ache, and T. S. McClintock Gene expression and specificity in the mature zone of the lobster olfactory organ. Physiol Genomics, April 13, 2006; 25(2): 224 - 233. [Abstract] [Full Text] [PDF]

				M. Iwai, Y. Tateishi, M. Hattori, A. Mizutani, T. Nakamura, A. Futatsugi, T. Inoue, T. Furuichi, T. Michikawa, and K. Mikoshiba Molecular Cloning of Mouse Type 2 and Type 3 Inositol 1,4,5-Trisphosphate Receptors and Identification of a Novel Type 2 Receptor Splice Variant J. Biol. Chem., March 18, 2005; 280(11): 10305 - 10317. [Abstract] [Full Text] [PDF]

				Y. V. Bobkov and B. W. Ache Pharmacological Properties and Functional Role of a TRP-Related Ion Channel in Lobster Olfactory Receptor Neurons J Neurophysiol, March 1, 2005; 93(3): 1372 - 1380. [Abstract] [Full Text] [PDF]

				R. Stepanyan, B. Hollins, S. E. Brock, and T. S. McClintock Primary Culture of Lobster (Homarus americanus) Olfactory Sensory Neurons Chem Senses, March 1, 2004; 29(3): 179 - 187. [Abstract] [Full Text] [PDF]

				Y. V. Bobkov and B. W. Ache Calcium Sensitivity of a Sodium-Activated Nonselective Cation Channel in Lobster Olfactory Receptor Neurons J Neurophysiol, November 1, 2003; 90(5): 2928 - 2940. [Abstract] [Full Text] [PDF]

				A. R. Cinelli, D. Wang, P. Chen, W. Liu, and M. Halpern Calcium Transients in the Garter Snake Vomeronasal Organ J Neurophysiol, March 1, 2002; 87(3): 1449 - 1472. [Abstract] [Full Text] [PDF]

				A. B. Zhainazarov, R. Doolin, J.-D. Herlihy, and B. W. Ache Odor-Stimulated Phosphatidylinositol 3-Kinase in Lobster Olfactory Receptor Cells J Neurophysiol, June 1, 2001; 85(6): 2537 - 2544. [Abstract] [Full Text] [PDF]

				G. Beutner, V. K. Sharma, D. R. Giovannucci, D. I. Yule, and S.-S. Sheu Identification of a Ryanodine Receptor in Rat Heart Mitochondria J. Biol. Chem., June 8, 2001; 276(24): 21482 - 21488. [Abstract] [Full Text] [PDF]