JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M910189199 on April 25, 2000

J. Biol. Chem., Vol. 275, Issue 27, 20496-20501, July 7, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/27/20496    most recent
M910189199v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vollmer, W.
Right arrow Articles by Tomasz, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vollmer, W.
Right arrow Articles by Tomasz, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The pgdA Gene Encodes for a Peptidoglycan N-Acetylglucosamine Deacetylase in Streptococcus pneumoniae*

Waldemar Vollmer and Alexander TomaszDagger

From The Rockefeller University, Laboratory of Microbiology, New York, New York 10021

Analytical work on the fractionation of the glycan strands of Streptococcus pneumoniae cell wall has led to the observation that an unusually high proportion of hexosamine units (over 80% of the glucosamine and 10% of the muramic acid residues) was not N-acetylated, explaining the resistance of the peptidoglycan to the hydrolytic action of lysozyme, a muramidase that cleaves in the glycan backbone. A gene, pgdA, was identified as encoding for the peptidoglycan N-acetylglucosamine deacetylase A with amino acid sequence similarity to fungal chitin deacetylases and rhizobial NodB chitooligosaccharide deacetylases. Pneumococci in which pgdA was inactivated by insertion duplication mutagenesis produced fully N-acetylated glycan and became hypersensitive to exogenous lysozyme in the stationary phase of growth. The pgdA gene may contribute to pneumococcal virulence by providing protection against host lysozyme, which is known to accumulate in high concentrations at infection sites.

* Partial support for this study was provided by National Institutes of Health Grant AI37275 and by the Irene Diamond Foundation. Sequencing of S. pneumoniae was accomplished with support from The Institute for Genomic Research, the National Institute of Allergy and Infectious Diseases, and the Merck Genome Research Institute.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ251472.

Dagger To whom correspondence should be addressed. Tel.: 212-327-8278; Fax: 212-327-8688; E-mail: tomasz@rockvax.rockefeller.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
L. Hebert, P. Courtin, R. Torelli, M. Sanguinetti, M.-P. Chapot-Chartier, Y. Auffray, and A. Benachour
Enterococcus faecalis Constitutes an Unusual Bacterial Model in Lysozyme Resistance
Infect. Immun., November 1, 2007; 75(11): 5390 - 5398.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. Meyrand, A. Boughammoura, P. Courtin, C. Mezange, A. Guillot, and M.-P. Chapot-Chartier
Peptidoglycan N-acetylglucosamine deacetylation decreases autolysis in Lactococcus lactis
Microbiology, October 1, 2007; 153(10): 3275 - 3285.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
C. Rennemeier, S. Hammerschmidt, S. Niemann, S. Inamura, U. Zahringer, and B. E. Kehrel
Thrombospondin-1 promotes cellular adherence of Gram-positive pathogens via recognition of peptidoglycan
FASEB J, October 1, 2007; 21(12): 3118 - 3132.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Veiga, C. Bulbarela-Sampieri, S. Furlan, A. Maisons, M.-P. Chapot-Chartier, M. Erkelenz, P. Mervelet, P. Noirot, D. Frees, O. P. Kuipers, et al.
SpxB Regulates O-Acetylation-dependent Resistance of Lactococcus lactis Peptidoglycan to Hydrolysis
J. Biol. Chem., July 6, 2007; 282(27): 19342 - 19354.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
L. I. Salzberg and J. D. Helmann
An Antibiotic-Inducible Cell Wall-Associated Protein That Protects Bacillus subtilis from Autolysis
J. Bacteriol., July 1, 2007; 189(13): 4671 - 4680.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
N. Fittipaldi, M. Gottschalk, G. Vanier, F. Daigle, and J. Harel
Use of Selective Capture of Transcribed Sequences To Identify Genes Preferentially Expressed by Streptococcus suis upon Interaction with Porcine Brain Microvascular Endothelial Cells
Appl. Envir. Microbiol., July 1, 2007; 73(13): 4359 - 4364.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Kovacs, A. Halfmann, I. Fedtke, M. Heintz, A. Peschel, W. Vollmer, R. Hakenbeck, and R. Bruckner
A Functional dlt Operon, Encoding Proteins Required for Incorporation of D-Alanine in Teichoic Acids in Gram-Positive Bacteria, Confers Resistance to Cationic Antimicrobial Peptides in Streptococcus pneumoniae.
J. Bacteriol., August 1, 2006; 188(16): 5797 - 5805.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
P. Courtin, G. Miranda, A. Guillot, F. Wessner, C. Mezange, E. Domakova, S. Kulakauskas, and M.-P. Chapot-Chartier
Peptidoglycan Structure Analysis of Lactococcus lactis Reveals the Presence of an L,D-Carboxypeptidase Involved in Peptidoglycan Maturation.
J. Bacteriol., July 1, 2006; 188(14): 5293 - 5298.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. E. Blair, A. W. Schuttelkopf, J. I. MacRae, and D. M. F. van Aalten
Structure and metal-dependent mechanism of peptidoglycan deacetylase, a streptococcal virulence factor
PNAS, October 25, 2005; 102(43): 15429 - 15434.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Psylinakis, I. G. Boneca, K. Mavromatis, A. Deli, E. Hayhurst, S. J. Foster, K. M. Varum, and V. Bouriotis
Peptidoglycan N-Acetylglucosamine Deacetylases from Bacillus cereus, Highly Conserved Proteins in Bacillus anthracis
J. Biol. Chem., September 2, 2005; 280(35): 30856 - 30863.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Mahapatra, T. Yagi, J. T. Belisle, B. J. Espinosa, P. J. Hill, M. R. McNeil, P. J. Brennan, and D. C. Crick
Mycobacterial Lipid II Is Composed of a Complex Mixture of Modified Muramyl and Peptide Moieties Linked to Decaprenyl Phosphate
J. Bacteriol., April 15, 2005; 187(8): 2747 - 2757.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. Fukushima, T. Kitajima, and J. Sekiguchi
A Polysaccharide Deacetylase Homologue, PdaA, in Bacillus subtilis Acts as an N-Acetylmuramic Acid Deacetylase In Vitro
J. Bacteriol., February 15, 2005; 187(4): 1287 - 1292.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. Benachour, C. Muller, M. Dabrowski-Coton, Y. Le Breton, J.-C. Giard, A. Rince, Y. Auffray, and A. Hartke
The Enterococcus faecalis SigV Protein Is an Extracytoplasmic Function Sigma Factor Contributing to Survival following Heat, Acid, and Ethanol Treatments
J. Bacteriol., February 1, 2005; 187(3): 1022 - 1035.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. B. Raymond, S. Mahapatra, D. C. Crick, and M. S. Pavelka Jr.
Identification of the namH Gene, Encoding the Hydroxylase Responsible for the N-Glycolylation of the Mycobacterial Peptidoglycan
J. Biol. Chem., January 7, 2005; 280(1): 326 - 333.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
T. Fukushima, T. Tanabe, H. Yamamoto, S. Hosoya, T. Sato, H. Yoshikawa, and J. Sekiguchi
Characterization of a Polysaccharide Deacetylase Gene Homologue (pdaB) on Sporulation of Bacillus subtilis
J. Biochem., September 1, 2004; 136(3): 283 - 291.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. E. Gilmore, D. Bandyopadhyay, A. M. Dean, S. D. Linnstaedt, and D. L. Popham
Production of Muramic {delta}-Lactam in Bacillus subtilis Spore Peptidoglycan
J. Bacteriol., January 1, 2004; 186(1): 80 - 89.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Antignac, J.-C. Rousselle, A. Namane, A. Labigne, M.-K. Taha, and I. G. Boneca
Detailed Structural Analysis of the Peptidoglycan of the Human Pathogen Neisseria meningitidis
J. Biol. Chem., August 22, 2003; 278(34): 31521 - 31528.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
W. Vollmer and A. Tomasz
Peptidoglycan N-Acetylglucosamine Deacetylase, a Putative Virulence Factor in Streptococcus pneumoniae
Infect. Immun., December 1, 2002; 70(12): 7176 - 7178.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. Fukushima, H. Yamamoto, A. Atrih, S. J. Foster, and J. Sekiguchi
A Polysaccharide Deacetylase Gene (pdaA) Is Required for Germination and for Production of Muramic {delta}-Lactam Residues in the Spore Cortex of Bacillus subtilis
J. Bacteriol., November 1, 2002; 184(21): 6007 - 6015.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. De Las Rivas, J. L. Garcia, R. Lopez, and P. Garcia
Purification and Polar Localization of Pneumococcal LytB, a Putative Endo-{beta}-N-Acetylglucosaminidase: the Chain-Dispersing Murein Hydrolase
J. Bacteriol., September 15, 2002; 184(18): 4988 - 5000.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. N. Neely, J. D. Pfeifer, and M. Caparon
Streptococcus-Zebrafish Model of Bacterial Pathogenesis
Infect. Immun., July 1, 2002; 70(7): 3904 - 3914.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
M.-R. Yen, N.-T. Lin, C.-H. Hung, K.-T. Choy, S.-F. Weng, and Y.-H. Tseng
oriC Region and Replication Termination Site, dif, of the Xanthomonas campestris pv. campestris 17 Chromosome
Appl. Envir. Microbiol., June 1, 2002; 68(6): 2924 - 2933.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. M. Levitz, S.-h. Nong, M. K. Mansour, C. Huang, and C. A. Specht
Molecular characterization of a mannoprotein with homology to chitin deacetylases that stimulates T cell responses to Cryptococcus neoformans
PNAS, August 10, 2001; (2001) 181331398.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. R. Filipe, M. G. Pinho, and A. Tomasz
Characterization of the murMN Operon Involved in the Synthesis of Branched Peptidoglycan Peptides in Streptococcus pneumoniae
J. Biol. Chem., September 1, 2000; 275(36): 27768 - 27774.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Monchois, C. Abergel, J. Sturgis, S. Jeudy, and J.-M. Claverie
Escherichia coli ykfE ORFan Gene Encodes a Potent Inhibitor of C-type Lysozyme
J. Biol. Chem., May 18, 2001; 276(21): 18437 - 18441.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. M. Levitz, S.-h. Nong, M. K. Mansour, C. Huang, and C. A. Specht
Molecular characterization of a mannoprotein with homology to chitin deacetylases that stimulates T cell responses to Cryptococcus neoformans
PNAS, August 28, 2001; 98(18): 10422 - 10427.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.