|
Originally published In Press as doi:10.1074/jbc.M002323200 on April 10, 2000
J. Biol. Chem., Vol. 275, Issue 27, 20693-20699, July 7, 2000
Sperm Motility Is Dependent on a Unique Isoform of the
Na,K-ATPase*
Alison L.
Woo,
Paul F.
James, and
Jerry B
Lingrel
From the Department of Molecular Genetics, Biochemistry, and
Microbiology, University of Cincinnati College of Medicine,
Cincinnati, Ohio 45267-0524
The Na,K-ATPase, a member of the P-type ATPases,
is composed of two subunits, and , and is responsible for
translocating Na+ out of the cell and K+
into the cell using the energy of hydrolysis of one molecule of ATP.
The electrochemical gradient it generates is necessary for many
cellular functions, including establishment of the plasma membrane
potential and transport of sugars and ions in and out of the cell.
Families of isoforms for both the and subunits have been
identified, and specific functional roles for individual isoforms are
just beginning to emerge. The 4 isoform is the most recently
identified Na,K-ATPase isoform, and its expression has been found
only in testis. Here we show that expression of the 4 isoform in
testis is localized to spermatozoa and that inhibition of this isoform
alone eliminates sperm motility. These data describe for the first time
a biological function for the 4 isoform of the Na,K-ATPase,
revealing a critical role for this isoform in sperm motility.
*
This work was supported by National Institutes of Health
Grants RO1HL28573 and PO1HL41496 (to J. B L.) and Training Grant T32HL07382 (to A. L. W.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence and reprint requests should be addressed:
Dept. of Molecular Genetics, Biochemistry, and Microbiology, University
of Cincinnati College of Medicine, 231 Bethesda Ave., Cincinnati, OH
45267-0524. E-mail: Jerry.Lingrel@uc.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
E.-Y. Gong, E. Park, H. J. Lee, and K. Lee
Expression of Atp8b3 in murine testis and its characterization as a testis specific P-type ATPase
Reproduction,
February 1, 2009;
137(2):
345 - 351.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. P. Morth, H. Poulsen, M. S Toustrup-Jensen, V. R. Schack, J. Egebjerg, J. P. Andersen, B. Vilsen, and P. Nissen
The structure of the Na+,K+-ATPase and mapping of isoform differences and disease-related mutations
Phil Trans R Soc B,
January 27, 2009;
364(1514):
217 - 227.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. G. Berry, S. Despa, W. Fuller, D. M. Bers, and M. J. Shattock
Differential distribution and regulation of mouse cardiac Na+/K+-ATPase {alpha}1 and {alpha}2 subunits in T-tubule and surface sarcolemmal membranes
Cardiovasc Res,
January 1, 2007;
73(1):
92 - 100.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. P. Masly, C. D. Jones, M. A. F. Noor, J. Locke, and H. A. Orr
Gene transposition as a cause of hybrid sterility in Drosophila.
Science,
September 8, 2006;
313(5792):
1448 - 1450.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Sanchez, A.-N. T. Nguyen, B. Timmerberg, J. S. Tash, and G. Blanco
The Na,K-ATPase {alpha}4 isoform from humans has distinct enzymatic properties and is important for sperm motility
Mol. Hum. Reprod.,
September 1, 2006;
12(9):
565 - 576.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. C. Thundathil, M. Anzar, and M. M. Buhr
Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation
Biol Reprod,
September 1, 2006;
75(3):
308 - 317.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Song, M. Y. Lee, S. P. Kinsey, D. J. Weber, and M. P. Blaustein
An N-terminal Sequence Targets and Tethers Na+ Pump {alpha}2 Subunits to Specialized Plasma Membrane Microdomains
J. Biol. Chem.,
May 5, 2006;
281(18):
12929 - 12940.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
I. Dostanic-Larson, J. N. Lorenz, J. W. Van Huysse, J. C. Neumann, A. E. Moseley, and J. B Lingrel
Physiological role of the {alpha}1- and {alpha}2-isoforms of the Na+-K+-ATPase and biological significance of their cardiac glycoside binding site
Am J Physiol Regulatory Integrative Comp Physiol,
March 1, 2006;
290(3):
R524 - R528.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Gur and H. Breitbart
Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes
Genes & Dev.,
February 15, 2006;
20(4):
411 - 416.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K Wagoner, G Sanchez, A-N Nguyen, G C Enders, and G Blanco
Different expression and activity of the {alpha}1 and {alpha}4 isoforms of the Na,K-ATPase during rat male germ cell ontogeny
Reproduction,
November 1, 2005;
130(5):
627 - 641.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
I. Dostanic-Larson, J. W. Van Huysse, J. N. Lorenz, and J. B Lingrel
From The Cover: The highly conserved cardiac glycoside binding site of Na,K-ATPase plays a role in blood pressure regulation
PNAS,
November 1, 2005;
102(44):
15845 - 15850.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T Sato, H Indo, Y Kawabata, T Kobayashi, S Suenaga, Y Iwashita, T Nitta, K Sugihara, and H. Majima
Thallium-201 chloride (Tl-201) accumulation and Na+/K+-ATPase expression in tumours of the head and neck
Dentomaxillofac. Radiol.,
July 1, 2005;
34(4):
212 - 217.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. W. Okunade, M. L. Miller, G. J. Pyne, R. L. Sutliff, K. T. O'Connor, J. C. Neumann, A. Andringa, D. A. Miller, V. Prasad, T. Doetschman, et al.
Targeted Ablation of Plasma Membrane Ca2+-ATPase (PMCA) 1 and 4 Indicates a Major Housekeeping Function for PMCA1 and a Critical Role in Hyperactivated Sperm Motility and Male Fertility for PMCA4
J. Biol. Chem.,
August 6, 2004;
279(32):
33742 - 33750.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
I. Dostanic, J. N. Lorenz, J. E. J. Schultz, I. L. Grupp, J. C. Neumann, M. A. Wani, and J. B Lingrel
The {alpha}2 Isoform of Na,K-ATPase Mediates Ouabain-induced Cardiac Inotropy in Mice
J. Biol. Chem.,
December 26, 2003;
278(52):
53026 - 53034.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. G. Richards, J. W. Semple, J. S. Bystriansky, and P. M. Schulte
Na+/K+-ATPase {alpha}-isoform switching in gills of rainbow trout (Oncorhynchus mykiss) during salinity transfer
J. Exp. Biol.,
December 15, 2003;
206(24):
4475 - 4486.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. He, D. A. Shelly, A. E. Moseley, P. F. James, J. H. James, R. J. Paul, and J. B. Lingrel
The {alpha}1- and {alpha}2-isoforms of Na-K-ATPase play different roles in skeletal muscle contractility
Am J Physiol Regulatory Integrative Comp Physiol,
September 1, 2001;
281(3):
R917 - R925.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. Longo, F. Scaglia, and Y. Wang
Insulin increases the turnover rate of Na+-K+-ATPase in human fibroblasts
Am J Physiol Cell Physiol,
April 1, 2001;
280(4):
C912 - C919.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Mansharamani, A. Hewetson, and B. S. Chilton
Cloning and Characterization of an Atypical Type IV P-type ATPase That Binds to the RING Motif of RUSH Transcription Factors
J. Biol. Chem.,
January 26, 2001;
276(5):
3641 - 3649.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Segall, S. E. Daly, and R. Blostein
Mechanistic Basis for Kinetic Differences between the Rat alpha 1, alpha 2, and alpha 3 Isoforms of the Na,K-ATPase
J. Biol. Chem.,
August 17, 2001;
276(34):
31535 - 31541.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|