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J. Biol. Chem., Vol. 275, Issue 27, 20693-20699, July 7, 2000
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From the Department of Molecular Genetics, Biochemistry, and
Microbiology, University of Cincinnati College of Medicine,
Cincinnati, Ohio 45267-0524
The Na,K-ATPase, a member of the P-type ATPases,
is composed of two subunits,
Sperm Motility Is Dependent on a Unique Isoform of the
Na,K-ATPase*
and 
, and is responsible for
translocating Na+ out of the cell and K+
into the cell using the energy of hydrolysis of one molecule of ATP.
The electrochemical gradient it generates is necessary for many
cellular functions, including establishment of the plasma membrane
potential and transport of sugars and ions in and out of the cell.
Families of isoforms for both the and subunits have been
identified, and specific functional roles for individual isoforms are
just beginning to emerge. The 4 isoform is the most recently
identified Na,K-ATPase isoform, and its expression has been found
only in testis. Here we show that expression of the 4 isoform in
testis is localized to spermatozoa and that inhibition of this isoform
alone eliminates sperm motility. These data describe for the first time
a biological function for the 4 isoform of the Na,K-ATPase,
revealing a critical role for this isoform in sperm motility.
*
This work was supported by National Institutes of Health
Grants RO1HL28573 and PO1HL41496 (to J. B L.) and Training Grant T32HL07382 (to A. L. W.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence and reprint requests should be addressed:
Dept. of Molecular Genetics, Biochemistry, and Microbiology, University
of Cincinnati College of Medicine, 231 Bethesda Ave., Cincinnati, OH
45267-0524. E-mail: Jerry.Lingrel@uc.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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